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Optimization of a liquid chromatographic separation for the simultaneous determination of four anthracyclines and their respective 13-S-dihydro metabolites

(2008) JOURNAL OF SEPARATION SCIENCE. 31(6-7). p.1042-1049
Author
Organization
Abstract
The optimization of a liquid chromatographic separation of four anthracyclines (doxorubicin, epirubicin, daunorubicin and idarubicin) and their respective 13-S-dihydro metabolites (doxorubicinol, epirubicinol, daunorubicinol and idarubicinol) is described. Inclusion of epidaunorubicin in the assay allows internal standardization. Potential chromatographic interference of aglycones (doxorubicinone, daunorubicinone, idarubicinone, doxorubicinolone, daunorubicinolone and idarubicinolone) was investigated and tackled. The analyses were performed on a C18 RP column and gradient elutions were performed with a mixture of 0.1% formic acid in water and 0.1% formic acid in ACN. The analytes were measured by fluorescence detection with an excitation wavelength of 480 nm. and emission wavelength of 555 nm, respectively. The separation will potentially allow a broad field of applications, ranging from therapeutic drug monitoring and kinetic studies in cancer patients to monitoring hospital personnel and effluents.
Keywords
ANTICANCER DRUGS, DAUNORUBICIN, MASS-SPECTROMETRY, HUMAN PLASMA, EPIRUBICIN, DOXORUBICIN, ADRIAMYCIN

Citation

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MLA
Maudens, Kristof, et al. “Optimization of a Liquid Chromatographic Separation for the Simultaneous Determination of Four Anthracyclines and Their Respective 13-S-Dihydro Metabolites.” JOURNAL OF SEPARATION SCIENCE, vol. 31, no. 6–7, 2008, pp. 1042–49, doi:10.1002/jssc.200700490.
APA
Maudens, K., Stove, C., & Lambert, W. (2008). Optimization of a liquid chromatographic separation for the simultaneous determination of four anthracyclines and their respective 13-S-dihydro metabolites. JOURNAL OF SEPARATION SCIENCE, 31(6–7), 1042–1049. https://doi.org/10.1002/jssc.200700490
Chicago author-date
Maudens, Kristof, Christophe Stove, and Willy Lambert. 2008. “Optimization of a Liquid Chromatographic Separation for the Simultaneous Determination of Four Anthracyclines and Their Respective 13-S-Dihydro Metabolites.” JOURNAL OF SEPARATION SCIENCE 31 (6–7): 1042–49. https://doi.org/10.1002/jssc.200700490.
Chicago author-date (all authors)
Maudens, Kristof, Christophe Stove, and Willy Lambert. 2008. “Optimization of a Liquid Chromatographic Separation for the Simultaneous Determination of Four Anthracyclines and Their Respective 13-S-Dihydro Metabolites.” JOURNAL OF SEPARATION SCIENCE 31 (6–7): 1042–1049. doi:10.1002/jssc.200700490.
Vancouver
1.
Maudens K, Stove C, Lambert W. Optimization of a liquid chromatographic separation for the simultaneous determination of four anthracyclines and their respective 13-S-dihydro metabolites. JOURNAL OF SEPARATION SCIENCE. 2008;31(6–7):1042–9.
IEEE
[1]
K. Maudens, C. Stove, and W. Lambert, “Optimization of a liquid chromatographic separation for the simultaneous determination of four anthracyclines and their respective 13-S-dihydro metabolites,” JOURNAL OF SEPARATION SCIENCE, vol. 31, no. 6–7, pp. 1042–1049, 2008.
@article{533007,
  abstract     = {{The optimization of a liquid chromatographic separation of four anthracyclines (doxorubicin, epirubicin, daunorubicin and idarubicin) and their respective 13-S-dihydro metabolites (doxorubicinol, epirubicinol, daunorubicinol and idarubicinol) is described. Inclusion of epidaunorubicin in the assay allows internal standardization. Potential chromatographic interference of aglycones (doxorubicinone, daunorubicinone, idarubicinone, doxorubicinolone, daunorubicinolone and idarubicinolone) was investigated and tackled. The analyses were performed on a C18 RP column and gradient elutions were performed with a mixture of 0.1% formic acid in water and 0.1% formic acid in ACN. The analytes were measured by fluorescence detection with an excitation wavelength of 480 nm. and emission wavelength of 555 nm, respectively. The separation will potentially allow a broad field of applications, ranging from therapeutic drug monitoring and kinetic studies in cancer patients to monitoring hospital personnel and effluents.}},
  author       = {{Maudens, Kristof and Stove, Christophe and Lambert, Willy}},
  issn         = {{1615-9306}},
  journal      = {{JOURNAL OF SEPARATION SCIENCE}},
  keywords     = {{ANTICANCER DRUGS,DAUNORUBICIN,MASS-SPECTROMETRY,HUMAN PLASMA,EPIRUBICIN,DOXORUBICIN,ADRIAMYCIN}},
  language     = {{eng}},
  number       = {{6-7}},
  pages        = {{1042--1049}},
  title        = {{Optimization of a liquid chromatographic separation for the simultaneous determination of four anthracyclines and their respective 13-S-dihydro metabolites}},
  url          = {{http://doi.org/10.1002/jssc.200700490}},
  volume       = {{31}},
  year         = {{2008}},
}

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