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The Tandem PDZ Protein Syntenin Interacts with the Aminoacyl tRNA Synthetase Complex in a Lysyl-tRNA Synthetase-Dependent Manner

(2008) JOURNAL OF PROTEOME RESEARCH. 7(11). p.4962-4973
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Abstract
Syntenin-1 is a tandem PDZ protein that binds a diverse array of signaling molecules that are often associated with cell adhesion and intracellular trafficking. With the use of a MS-based functional proteornics approach, we identified several members of the aminoacyl-tRNA synthetase macromolecular (ARS) complex in a syntenin-1 pull down assay. Interaction of these proteins with syntenin-1 was confirmed by co-immunoprecipitation from cultured cells. We demonstrate a direct interaction of syntenin-1 with lysyl-tRNA synthetase (KRS), which contains a PDZ binding motif at its C-terminus. This motif is important for the interaction of the entire complex with syntenin-1. A point mutation in the PDZ2 domain of syntenin-1 abrogates interaction with KRS. As a result, other components of the ARS complex no longer co-immunoprecipitate with syntenin-1. We further show that syntenin-1 regulates KRS activity. These findings suggest that syntenin-1 is an adaptor modulating the activity of KRS.
Keywords
TRANSLATION, IDENTIFICATION, GENE, DOMAINS, CELL INVASION, Syntenin, Signal transduction, PDZ, Functional proteomics, tRNA synthetase, IMMUNODEFICIENCY-VIRUS TYPE-1, MELANOMA METASTASIS, CRYSTAL-STRUCTURE, BINDING MOTIFS, PLASMA-MEMBRANE

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Chicago
Meerschaert, Kris, Eline Remue, Ariane De Ganck, An Staes, Ciska Boucherie, Kris Gevaert, Joël Vandekerckhove, Lawrence Kleiman, and Jan Gettemans. 2008. “The Tandem PDZ Protein Syntenin Interacts with the Aminoacyl tRNA Synthetase Complex in a Lysyl-tRNA Synthetase-Dependent Manner.” Journal of Proteome Research 7 (11): 4962–4973.
APA
Meerschaert, K., Remue, E., De Ganck, A., Staes, A., Boucherie, C., Gevaert, K., Vandekerckhove, J., et al. (2008). The Tandem PDZ Protein Syntenin Interacts with the Aminoacyl tRNA Synthetase Complex in a Lysyl-tRNA Synthetase-Dependent Manner. JOURNAL OF PROTEOME RESEARCH, 7(11), 4962–4973.
Vancouver
1.
Meerschaert K, Remue E, De Ganck A, Staes A, Boucherie C, Gevaert K, et al. The Tandem PDZ Protein Syntenin Interacts with the Aminoacyl tRNA Synthetase Complex in a Lysyl-tRNA Synthetase-Dependent Manner. JOURNAL OF PROTEOME RESEARCH. WASHINGTON, DC 20036 USA: AMER CHEMICAL SOC; 2008;7(11):4962–73.
MLA
Meerschaert, Kris, Eline Remue, Ariane De Ganck, et al. “The Tandem PDZ Protein Syntenin Interacts with the Aminoacyl tRNA Synthetase Complex in a Lysyl-tRNA Synthetase-Dependent Manner.” JOURNAL OF PROTEOME RESEARCH 7.11 (2008): 4962–4973. Print.
@article{532910,
  abstract     = {Syntenin-1 is a tandem PDZ protein that binds a diverse array of signaling molecules that are often associated with cell adhesion and intracellular trafficking. With the use of a MS-based functional proteornics approach, we identified several members of the aminoacyl-tRNA synthetase macromolecular (ARS) complex in a syntenin-1 pull down assay. Interaction of these proteins with syntenin-1 was confirmed by co-immunoprecipitation from cultured cells. We demonstrate a direct interaction of syntenin-1 with lysyl-tRNA synthetase (KRS), which contains a PDZ binding motif at its C-terminus. This motif is important for the interaction of the entire complex with syntenin-1. A point mutation in the PDZ2 domain of syntenin-1 abrogates interaction with KRS. As a result, other components of the ARS complex no longer co-immunoprecipitate with syntenin-1. We further show that syntenin-1 regulates KRS activity. These findings suggest that syntenin-1 is an adaptor modulating the activity of KRS.},
  author       = {Meerschaert, Kris and Remue, Eline and De Ganck, Ariane and Staes, An and Boucherie, Ciska and Gevaert, Kris and Vandekerckhove, Jo{\"e}l and Kleiman, Lawrence and Gettemans, Jan},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keyword      = {TRANSLATION,IDENTIFICATION,GENE,DOMAINS,CELL INVASION,Syntenin,Signal transduction,PDZ,Functional proteomics,tRNA synthetase,IMMUNODEFICIENCY-VIRUS TYPE-1,MELANOMA METASTASIS,CRYSTAL-STRUCTURE,BINDING MOTIFS,PLASMA-MEMBRANE},
  language     = {eng},
  number       = {11},
  pages        = {4962--4973},
  publisher    = {AMER CHEMICAL SOC},
  title        = {The Tandem PDZ Protein Syntenin Interacts with the Aminoacyl tRNA Synthetase Complex in a Lysyl-tRNA Synthetase-Dependent Manner},
  url          = {http://dx.doi.org/10.1021/pr800325u},
  volume       = {7},
  year         = {2008},
}

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