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Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes

Barbara Geusens (UGent) , Jo Lambert (UGent) , Stefaan De Smedt (UGent) , Kevin Buyens (UGent) , Niek Sanders (UGent) and Mireille Van Gele (UGent)
(2009) Journal of Controlled Release. 133(3). p.214-220
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Abstract
The aim of this work was to develop a system that can deliver siRNA into cells present in the human epidermis. More specifically, we wanted to block the expression of a specific Myosin Va exon F containing isoform that is physiologically involved in melanosome transport in human melanocytes. Therefore, we prepared and investigated the capacity of ultradeformable cationic liposomes (UCLs) to deliver siRNA in hard-to-transfect human primary melanocytes. UCLs were formulated from different w:w ratios (6:1, 8:1 and 10:1) of the cationic lipid 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and the edge activator sodium cholate. Subsequently, UCL/siRNA complexes were prepared and their particle size, surface charge, deformability, cytotoxicity, transfection efficiency and long-term stability were tested. The best results were obtained with UCLs composed of a DOTAP/NaChol ratio of 6:1 (w:w) which are promising for future in vivo experiments.
Keywords
VESICLES, ADULT MICE, INTACT SKIN, TOPICAL APPLICATION, IN-VIVO, TRIAMCINOLONE-ACETONIDE, TRANSDERMAL DELIVERY, TRANSFECTION EFFICIENCY, DRUG CARRIERS, CRYO-ELECTRON MICROSCOPY, siRNA, Melanocytes, Skin, Ultradeformable liposomes, Delivery vehicle

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Chicago
Geusens, Barbara, Jo Lambert, Stefaan De Smedt, Kevin Buyens, Niek Sanders, and Mireille Van Gele. 2009. “Ultradeformable Cationic Liposomes for Delivery of Small Interfering RNA (siRNA) into Human Primary Melanocytes.” Journal of Controlled Release 133 (3): 214–220.
APA
Geusens, B., Lambert, J., De Smedt, S., Buyens, K., Sanders, N., & Van Gele, M. (2009). Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes. Journal of Controlled Release, 133(3), 214–220.
Vancouver
1.
Geusens B, Lambert J, De Smedt S, Buyens K, Sanders N, Van Gele M. Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes. Journal of Controlled Release. Elsevier; 2009;133(3):214–20.
MLA
Geusens, Barbara, Jo Lambert, Stefaan De Smedt, et al. “Ultradeformable Cationic Liposomes for Delivery of Small Interfering RNA (siRNA) into Human Primary Melanocytes.” Journal of Controlled Release 133.3 (2009): 214–220. Print.
@article{529736,
  abstract     = {The aim of this work was to develop a system that can deliver siRNA into cells present in the human epidermis. More specifically, we wanted to block the expression of a specific Myosin Va exon F containing isoform that is physiologically involved in melanosome transport in human melanocytes. Therefore, we prepared and investigated the capacity of ultradeformable cationic liposomes (UCLs) to deliver siRNA in hard-to-transfect human primary melanocytes. UCLs were formulated from different w:w ratios (6:1, 8:1 and 10:1) of the cationic lipid 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) and the edge activator sodium cholate. Subsequently, UCL/siRNA complexes were prepared and their particle size, surface charge, deformability, cytotoxicity, transfection efficiency and long-term stability were tested. The best results were obtained with UCLs composed of a DOTAP/NaChol ratio of 6:1 (w:w) which are promising for future in vivo experiments.},
  author       = {Geusens, Barbara and Lambert, Jo and De Smedt, Stefaan and Buyens, Kevin and Sanders, Niek and Van Gele, Mireille},
  issn         = {0168-3659},
  journal      = {Journal of Controlled Release},
  language     = {eng},
  number       = {3},
  pages        = {214--220},
  publisher    = {Elsevier},
  title        = {Ultradeformable cationic liposomes for delivery of small interfering RNA (siRNA) into human primary melanocytes},
  url          = {http://dx.doi.org/10.1016/j.jconrel.2008.10.003},
  volume       = {133},
  year         = {2009},
}

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