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How the fas locus contributes to Rhodococcus fascians cytokinin production: an in-depth molecular and biochemical analysis

Ine Pertry UGent (2009)
abstract
The fine-tuned balance of plant regulators plays a key role in growth and development of plants. Many plant-associated bacteria can influence their hosts either by modulating phytohormone production or by producing phytohormones themselves. The Actinomycete Rhodococcus fascians provokes the formation of differentiated leafy galls consisting of numerous shoot primordia that are inhibited in further outgrowth. Based on the shooty phenotype and the presence of an ipt gene on the linear virulence plasmid of R. fascians D188, the role for cytokinins in the pathology had been anticipated for a long time. Subsequent studies identified and characterized the fas operon as a key genetic determinant of virulence and likely cytokinin biosynthesis. Nevertheless, many aspects concerning regulation of fas gene expression, Fas protein function, and, importantly, the encoded cytokinin biosynthetic pathway and the identity of the produced morphogens remained to be uncovered. Therefore, the main objectives of this research were to identify the bacterial cytokinins responsible for the R. fascians pathology, to unravel how they exerted their function, and to elucidate the role of the fas locus and its expression in their production. In conclusion, our data have largely uncovered the role of cytokinins and the fas locus in the R. fascians pathology: the continuous challenge with defined ratios of synergistically acting cytokinins eventually defeats nearly all plants and transforms them into shooty niches. Many intriguing questions derived from the novel insights obtained during this work remain to be answered. Nevertheless, we feel that the results presented here have shed some light on the remaining secrets of this fascinating pathogen.
Please use this url to cite or link to this publication:
author
promoter
UGent and UGent
organization
year
type
dissertation (monograph)
subject
pages
170 pages
publisher
Ghent University
place of publication
Gent
defense location
Gent
defense date
2009-03-06 14:00
language
English
UGent publication?
yes
classification
D1
copyright statement
I have retained and own the full copyright for this publication
id
529624
handle
http://hdl.handle.net/1854/LU-529624
alternative location
http://lib.ugent.be/fulltxt/RUG01/001/323/505/RUG01-001323505_2010_0001_AC.pdf
date created
2009-03-24 15:46:57
date last changed
2009-03-25 14:38:02
@phdthesis{529624,
  abstract     = {The fine-tuned balance of plant regulators plays a key role in growth and development of plants. Many plant-associated bacteria can influence their hosts either by modulating phytohormone production or by producing phytohormones themselves. The Actinomycete Rhodococcus fascians provokes the formation of differentiated leafy galls consisting of numerous shoot primordia that are inhibited in further outgrowth. Based on the shooty phenotype and the presence of an ipt gene on the linear virulence plasmid of R. fascians D188, the role for cytokinins in the pathology had been anticipated for a long time. Subsequent studies identified and characterized the fas operon as a key genetic determinant of virulence and likely cytokinin biosynthesis. Nevertheless, many aspects concerning regulation of fas gene expression, Fas protein function, and, importantly, the encoded cytokinin biosynthetic pathway and the identity of the produced morphogens remained to be uncovered. Therefore, the main objectives of this research were to identify the bacterial cytokinins responsible for the R. fascians pathology, to unravel how they exerted their function, and to elucidate the role of the fas locus and its expression in their production. In conclusion, our data have largely uncovered the role of cytokinins and the fas locus in the R. fascians pathology: the continuous challenge with defined ratios of synergistically acting cytokinins eventually defeats nearly all plants and transforms them into shooty niches. Many intriguing questions derived from the novel insights obtained during this work remain to be answered. Nevertheless, we feel that the results presented here have shed some light on the remaining secrets of this fascinating pathogen.},
  author       = {Pertry, Ine},
  language     = {eng},
  pages        = {170},
  publisher    = {Ghent University},
  school       = {Ghent University},
  title        = {How the fas locus contributes to Rhodococcus fascians cytokinin production: an in-depth molecular and biochemical analysis},
  url          = {http://lib.ugent.be/fulltxt/RUG01/001/323/505/RUG01-001323505\_2010\_0001\_AC.pdf},
  year         = {2009},
}

Chicago
Pertry, Ine. 2009. “How the Fas Locus Contributes to Rhodococcus Fascians Cytokinin Production: An In-depth Molecular and Biochemical Analysis”. Gent: Ghent University.
APA
Pertry, I. (2009). How the fas locus contributes to Rhodococcus fascians cytokinin production: an in-depth molecular and biochemical analysis. Ghent University, Gent.
Vancouver
1.
Pertry I. How the fas locus contributes to Rhodococcus fascians cytokinin production: an in-depth molecular and biochemical analysis. [Gent]: Ghent University; 2009.
MLA
Pertry, Ine. “How the Fas Locus Contributes to Rhodococcus Fascians Cytokinin Production: An In-depth Molecular and Biochemical Analysis.” 2009 : n. pag. Print.