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Chemopreventive effects from prebiotic inulin towards microbial 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine bioactivation

Lynn Vanhaecke (UGent) , Charlotte Grootaert (UGent) , Willy Verstraete (UGent) and Tom Van de Wiele (UGent)
(2009) JOURNAL OF APPLIED MICROBIOLOGY. 106(2). p.474-485
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Abstract
Using a Simulator of the Human Intestinal Microbial Ecosystem (SHIME), we investigated the chemopreventive potential of prebiotic chicory inulin towards the in vitro bioactivation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) by human intestinal microbiota. HPLC data revealed that inulin significantly decreased the formation of the genotoxic PhIP-M1 metabolite, with the highest inhibitory activity in the colon ascendens (87% decrease). Interestingly, this chemopreventive effect correlated with alterations of bacterial community composition and metabolism in the different colon compartments. Conventional culture-based techniques and PCR-DGGE analysis on the SHIME colon suspension revealed significant bifidogenic effects during inulin treatment, whereas the overall microbial community kept relatively unchanged. Additionally, the production of short-chain fatty acids increased with 12%, 3% and 7%, while ammonia concentrations decreased with 3%, 4% and 3% in the ascending, transverse and descending colon compartments, respectively. These results indicate that the prebiotic effects from inulin may also purport protective effects towards microbial PhIP bioactivation. As the colonic microbiota may contribute significantly to the carcinogenic potential of PhIP, the search for dietary constituents that decrease the formation of this harmful metabolite, may help in preventing its risk towards human health.
Keywords
heterocyclic amines, cancer, genotoxic, human colonic bacteria, SHIME

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MLA
Vanhaecke, Lynn, Charlotte Grootaert, Willy Verstraete, et al. “Chemopreventive Effects from Prebiotic Inulin Towards Microbial 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine Bioactivation.” JOURNAL OF APPLIED MICROBIOLOGY 106.2 (2009): 474–485. Print.
APA
Vanhaecke, Lynn, Grootaert, C., Verstraete, W., & Van de Wiele, T. (2009). Chemopreventive effects from prebiotic inulin towards microbial 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine bioactivation. JOURNAL OF APPLIED MICROBIOLOGY, 106(2), 474–485.
Chicago author-date
Vanhaecke, Lynn, Charlotte Grootaert, Willy Verstraete, and Tom Van de Wiele. 2009. “Chemopreventive Effects from Prebiotic Inulin Towards Microbial 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine Bioactivation.” Journal of Applied Microbiology 106 (2): 474–485.
Chicago author-date (all authors)
Vanhaecke, Lynn, Charlotte Grootaert, Willy Verstraete, and Tom Van de Wiele. 2009. “Chemopreventive Effects from Prebiotic Inulin Towards Microbial 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine Bioactivation.” Journal of Applied Microbiology 106 (2): 474–485.
Vancouver
1.
Vanhaecke L, Grootaert C, Verstraete W, Van de Wiele T. Chemopreventive effects from prebiotic inulin towards microbial 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine bioactivation. JOURNAL OF APPLIED MICROBIOLOGY. Oxford, UK: Blackwell; 2009;106(2):474–85.
IEEE
[1]
L. Vanhaecke, C. Grootaert, W. Verstraete, and T. Van de Wiele, “Chemopreventive effects from prebiotic inulin towards microbial 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine bioactivation,” JOURNAL OF APPLIED MICROBIOLOGY, vol. 106, no. 2, pp. 474–485, 2009.
@article{528789,
  abstract     = {Using a Simulator of the Human Intestinal Microbial Ecosystem (SHIME), we investigated the chemopreventive potential of prebiotic chicory inulin towards the in vitro bioactivation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) by human intestinal microbiota.
HPLC data revealed that inulin significantly decreased the formation of the genotoxic PhIP-M1 metabolite, with the highest inhibitory activity in the colon ascendens (87% decrease). Interestingly, this chemopreventive effect correlated with alterations of bacterial community composition and metabolism in the different colon compartments. Conventional culture-based techniques and PCR-DGGE analysis on the SHIME colon suspension revealed significant bifidogenic effects during inulin treatment, whereas the overall microbial community kept relatively unchanged. Additionally, the production of short-chain fatty acids increased with 12%, 3% and 7%, while ammonia concentrations decreased with 3%, 4% and 3% in the ascending, transverse and descending colon compartments, respectively.

These results indicate that the prebiotic effects from inulin may also purport protective effects towards microbial PhIP bioactivation.

As the colonic microbiota may contribute significantly to the carcinogenic potential of PhIP, the search for dietary constituents that decrease the formation of this harmful metabolite, may help in preventing its risk towards human health.},
  author       = {Vanhaecke, Lynn and Grootaert, Charlotte and Verstraete, Willy and Van de Wiele, Tom},
  issn         = {1364-5072},
  journal      = {JOURNAL OF APPLIED MICROBIOLOGY},
  keywords     = {heterocyclic amines,cancer,genotoxic,human colonic bacteria,SHIME},
  language     = {eng},
  number       = {2},
  pages        = {474--485},
  publisher    = {Blackwell},
  title        = {Chemopreventive effects from prebiotic inulin towards microbial 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine bioactivation},
  url          = {http://dx.doi.org/10.1111/j.1365-2672.2008.04015.x},
  volume       = {106},
  year         = {2009},
}

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