Ghent University Academic Bibliography

Advanced

A bioanalytical method for the proteome wide display and analysis of protein complexes from whole plant cell lysates

Noor Remmerie, Luc Roef, Eveline Van De Slijke UGent, Jelle Van Leene UGent, Geert Persiau UGent, Dominique Eeckhout UGent, Hilde Stals UGent, Kris Laukens, Filip Lemière and Eddy Esmans, et al. (2009) PROTEOMICS. 9(3). p.598-609
abstract
While protein interaction studies and protein network modeling come to the forefront, the isolation and identification of protein complexes in a cellular context remains a major challenge for plant science. To this end, a nondenaturing extraction procedure was optimized for plant whole cell matrices and the combined use of gel filtration and BN-PAGE for the separation of protein complexes was studied. Hyphenation to denaturing electrophoresis and mass spectrometric analysis allows for the simultaneous identification of multiple (previously unidentified) protein interactions in single samples. The reliability and efficacy of the technique was confirmed (i) by the identification of well-studied plant protein complexes, (ii) by the presence of nonplant interologs for several of the novel complexes (iii) by presenting physical evidence of previously hypothetical plant protein interactions and (iv) by the confirmation of found interactions using co-IP. Furthermore practical issues concerning the use of this 2-D BN/SDS-PAGE display method for the analysis of protein-protein interactions are discussed.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS-THALIANA, AFFINITY PURIFICATION, MASS, IDENTIFICATION, CHLOROPLASTS, ISOFORMS, DIPHOSPHATE KINASE, BLUE NATIVE ELECTROPHORESIS, CYTOSOLIC GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE, POLYACRYLAMIDE-GEL ELECTROPHORESIS, Protein complexes, Blue native gel electrophoresis, Nicotiana tabacum cv. bright yellow-2
journal title
PROTEOMICS
Proteomics
volume
9
issue
3
pages
598 - 609
Web of Science type
Article
Web of Science id
000263578600010
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
4.426 (2009)
JCR rank
11/65 (2009)
JCR quartile
1 (2009)
ISSN
1615-9853
DOI
10.1002/pmic.200800100
language
English
UGent publication?
yes
classification
A1
additional info
correction published in Proteomics (2009) 9(5), 1416 ; DOI 10.1002/pmic.200990016, states: Professor Geert De Jaeger and Dr. Erwin Witters share the senior authorship of this contribution.
copyright statement
I have transferred the copyright for this publication to the publisher
id
528497
handle
http://hdl.handle.net/1854/LU-528497
date created
2009-03-23 16:39:49
date last changed
2013-10-17 13:37:11
@article{528497,
  abstract     = {While protein interaction studies and protein network modeling come to the forefront, the isolation and identification of protein complexes in a cellular context remains a major challenge for plant science. To this end, a nondenaturing extraction procedure was optimized for plant whole cell matrices and the combined use of gel filtration and BN-PAGE for the separation of protein complexes was studied. Hyphenation to denaturing electrophoresis and mass spectrometric analysis allows for the simultaneous identification of multiple (previously unidentified) protein interactions in single samples. The reliability and efficacy of the technique was confirmed (i) by the identification of well-studied plant protein complexes, (ii) by the presence of nonplant interologs for several of the novel complexes (iii) by presenting physical evidence of previously hypothetical plant protein interactions and (iv) by the confirmation of found interactions using co-IP. Furthermore practical issues concerning the use of this 2-D BN/SDS-PAGE display method for the analysis of protein-protein interactions are discussed.},
  author       = {Remmerie, Noor and Roef, Luc and Van De Slijke, Eveline and Van Leene, Jelle and Persiau, Geert and Eeckhout, Dominique and Stals, Hilde and Laukens, Kris and Lemi{\`e}re, Filip and Esmans, Eddy and Van Onckelen, Harry and Inz{\'e}, Dirk and De Jaeger, Geert and Witters, Erwin},
  issn         = {1615-9853},
  journal      = {PROTEOMICS},
  keyword      = {ARABIDOPSIS-THALIANA,AFFINITY PURIFICATION,MASS,IDENTIFICATION,CHLOROPLASTS,ISOFORMS,DIPHOSPHATE KINASE,BLUE NATIVE ELECTROPHORESIS,CYTOSOLIC GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE,POLYACRYLAMIDE-GEL ELECTROPHORESIS,Protein complexes,Blue native gel electrophoresis,Nicotiana tabacum cv. bright yellow-2},
  language     = {eng},
  number       = {3},
  pages        = {598--609},
  title        = {A bioanalytical method for the proteome wide display and analysis of protein complexes from whole plant cell lysates},
  url          = {http://dx.doi.org/10.1002/pmic.200800100},
  volume       = {9},
  year         = {2009},
}

Chicago
Remmerie, Noor, Luc Roef, Eveline Van De Slijke, Jelle Van Leene, Geert Persiau, Dominique Eeckhout, Hilde Stals, et al. 2009. “A Bioanalytical Method for the Proteome Wide Display and Analysis of Protein Complexes from Whole Plant Cell Lysates.” Proteomics 9 (3): 598–609.
APA
Remmerie, N., Roef, L., Van De Slijke, E., Van Leene, J., Persiau, G., Eeckhout, D., Stals, H., et al. (2009). A bioanalytical method for the proteome wide display and analysis of protein complexes from whole plant cell lysates. PROTEOMICS, 9(3), 598–609.
Vancouver
1.
Remmerie N, Roef L, Van De Slijke E, Van Leene J, Persiau G, Eeckhout D, et al. A bioanalytical method for the proteome wide display and analysis of protein complexes from whole plant cell lysates. PROTEOMICS. 2009;9(3):598–609.
MLA
Remmerie, Noor, Luc Roef, Eveline Van De Slijke, et al. “A Bioanalytical Method for the Proteome Wide Display and Analysis of Protein Complexes from Whole Plant Cell Lysates.” PROTEOMICS 9.3 (2009): 598–609. Print.