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Expression of galectins-1,-3 and-4 varies with strain and type of experimental colitis in mice

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Abstract
Galectins are increasingly the focus of biomedical research. Although they are involved at different stages in inflammation, data on galectins in colitis remain scarce. The aim of this study was to determine and compare the expression of galectins in acute and chronic experimental colitis in mice. Immunohistochemistry for galectins-1, -3 and -4 was performed on colon tissue from C57BL/6 and BALB/c mice with acute dextran sodium sulphate colitis and from 129 Sv/Ev IL-10 knock-out (IL-10(-/-)) mice. From these three mouse strains, we first detected major differences in galectin expression related to the genetic background in the control animals. With regard to inflammation, chronic colitis in IL-10(-/-) mice was associated with increased galectin-4 expression; in contrast with the two other models, no galectin-1 and -3 alterations were observed in IL-10(-/-) mice. Acute colitis in C57BL/6 and BALB/c mice showed increased galectin-3 expression in the lamina propria and the crypt epithelium, together with a decreased nuclear expression. These results suggest an involvement of galectins in the development and perpetuation of colonic inflammation and illustrate that the choice of the mouse strain for studying galectins might influence the outcome of the experiments.
Keywords
T-CELLS, MOUSE, CARBOHYDRATE-BINDING PROTEIN-35, CHRONIC INTESTINAL INFLAMMATION, FAMILY, LECTIN, LOCALIZATION, HUMAN COLON-CANCER, CELL-MIGRATION, CROHNS-DISEASE

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MLA
Mathieu, Anne, Nathalie Nagy, Christine Decaestecker, et al. “Expression of Galectins-1,-3 and-4 Varies with Strain and Type of Experimental Colitis in Mice.” INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY 89.6 (2008): 438–446. Print.
APA
Mathieu, A., Nagy, N., Decaestecker, C., Ferdinande, L., Vandenbroucke, K., Rottiers, P., Cuvelier, C., et al. (2008). Expression of galectins-1,-3 and-4 varies with strain and type of experimental colitis in mice. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 89(6), 438–446.
Chicago author-date
Mathieu, Anne, Nathalie Nagy, Christine Decaestecker, Liesbeth Ferdinande, Klaas Vandenbroucke, Pieter Rottiers, Claude Cuvelier, Isabelle Salmon, and Pieter Demetter. 2008. “Expression of Galectins-1,-3 and-4 Varies with Strain and Type of Experimental Colitis in Mice.” International Journal of Experimental Pathology 89 (6): 438–446.
Chicago author-date (all authors)
Mathieu, Anne, Nathalie Nagy, Christine Decaestecker, Liesbeth Ferdinande, Klaas Vandenbroucke, Pieter Rottiers, Claude Cuvelier, Isabelle Salmon, and Pieter Demetter. 2008. “Expression of Galectins-1,-3 and-4 Varies with Strain and Type of Experimental Colitis in Mice.” International Journal of Experimental Pathology 89 (6): 438–446.
Vancouver
1.
Mathieu A, Nagy N, Decaestecker C, Ferdinande L, Vandenbroucke K, Rottiers P, et al. Expression of galectins-1,-3 and-4 varies with strain and type of experimental colitis in mice. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY. OXON, ENGLAND: BLACKWELL PUBLISHING; 2008;89(6):438–46.
IEEE
[1]
A. Mathieu et al., “Expression of galectins-1,-3 and-4 varies with strain and type of experimental colitis in mice,” INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, vol. 89, no. 6, pp. 438–446, 2008.
@article{527471,
  abstract     = {Galectins are increasingly the focus of biomedical research. Although they are involved at different stages in inflammation, data on galectins in colitis remain scarce. The aim of this study was to determine and compare the expression of galectins in acute and chronic experimental colitis in mice. Immunohistochemistry for galectins-1, -3 and -4 was performed on colon tissue from C57BL/6 and BALB/c mice with acute dextran sodium sulphate colitis and from 129 Sv/Ev IL-10 knock-out (IL-10(-/-)) mice. From these three mouse strains, we first detected major differences in galectin expression related to the genetic background in the control animals. With regard to inflammation, chronic colitis in IL-10(-/-) mice was associated with increased galectin-4 expression; in contrast with the two other models, no galectin-1 and -3 alterations were observed in IL-10(-/-) mice. Acute colitis in C57BL/6 and BALB/c mice showed increased galectin-3 expression in the lamina propria and the crypt epithelium, together with a decreased nuclear expression. These results suggest an involvement of galectins in the development and perpetuation of colonic inflammation and illustrate that the choice of the mouse strain for studying galectins might influence the outcome of the experiments.},
  author       = {Mathieu, Anne and Nagy, Nathalie and Decaestecker, Christine and Ferdinande, Liesbeth and Vandenbroucke, Klaas and Rottiers, Pieter and Cuvelier, Claude and Salmon, Isabelle and Demetter, Pieter},
  issn         = {0959-9673},
  journal      = {INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY},
  keywords     = {T-CELLS,MOUSE,CARBOHYDRATE-BINDING PROTEIN-35,CHRONIC INTESTINAL INFLAMMATION,FAMILY,LECTIN,LOCALIZATION,HUMAN COLON-CANCER,CELL-MIGRATION,CROHNS-DISEASE},
  language     = {eng},
  number       = {6},
  pages        = {438--446},
  publisher    = {BLACKWELL PUBLISHING},
  title        = {Expression of galectins-1,-3 and-4 varies with strain and type of experimental colitis in mice},
  url          = {http://dx.doi.org/10.1111/J.1365-2613.2008.00598.X},
  volume       = {89},
  year         = {2008},
}

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