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Changes in rumen biohydrogenation intermediates and ciliate protozoa diversity after algae supplementation to dairy cattle

Charlotte Boeckaert UGent, Veerle Fievez UGent, Dries Van Hecke, Willy Verstraete UGent and Nico Boon UGent (2007) EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. 109(8). p.767-777
abstract
During an in vivo trial with four rumen-fistulated dairy cows, the effect of dietary supplementation of docosahexaenoic acid (DHA; 22:6 n-3)-enriched microalgae (Schizochytrium sp., 2% on feed intake) on the fatty acid metabolism and microbial community in the rumen was investigated. Algal supplementation resulted in lower concentrations of saturated fatty acids while the concentrations of monounsaturated and polyunsaturated fatty acids increased as compared to the control treatment. An incomplete biohydrogenation of linoleic (18:2 n-6) and linolenic (18:3 n-3) acid was observed, resulting in a strong accumulation of 18:1 t11, 18:1t10 and 18:2 t11c15. Denaturing gradient gel electrophoresis (DGGE) was performed to study the rumen bacterial and ciliate population. Algal supplementation provoked changes in both communities. Ciliate DGGE profiles suggested a decreased abundance of Isotricha prostoma and I. intestinalis and some species of Epidinium caudatum, Eudiplodinium maggii and Diplodinium dentaturn in the rumen of algae-fed cows. Moreover, quantitative PCR indicated that incomplete biohydrogenation through algal addition was associated with decreased ciliate numbers. The simultaneous accumulation of hydrogenation intermediates and the disappearance of ciliates suggest that ciliates and/or their associated bacteria could play a role in rumen biohydrogenation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CONJUGATED LINOLEIC-ACID, qPCR, microalgae, 18 : 1 trans fatty acids, protozoa, UNSATURATED FATTY-ACIDS, biohydrogenation, GRADIENT GEL-ELECTROPHORESIS, LINSEED OIL SUPPLEMENTATION, 16S RIBOSOMAL-RNA, FED FISH-OIL, LIPID-METABOLISM, STEARIC-ACID, BUTYRIVIBRIO-FIBRISOLVENS, MICROBIAL COMMUNITY
journal title
EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Eur. J. Lipid Sci. Technol.
volume
109
issue
8
pages
767 - 777
Web of Science type
Article
Web of Science id
000249057900004
JCR category
FOOD SCIENCE & TECHNOLOGY
JCR impact factor
0.911 (2007)
JCR rank
52/102 (2007)
JCR quartile
3 (2007)
ISSN
1438-7697
DOI
10.1002/ejlt.200700052
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
527379
handle
http://hdl.handle.net/1854/LU-527379
date created
2009-03-22 22:10:13
date last changed
2010-06-15 14:45:03
@article{527379,
  abstract     = {During an in vivo trial with four rumen-fistulated dairy cows, the effect of dietary supplementation of docosahexaenoic acid (DHA; 22:6 n-3)-enriched microalgae (Schizochytrium sp., 2\% on feed intake) on the fatty acid metabolism and microbial community in the rumen was investigated. Algal supplementation resulted in lower concentrations of saturated fatty acids while the concentrations of monounsaturated and polyunsaturated fatty acids increased as compared to the control treatment. An incomplete biohydrogenation of linoleic (18:2 n-6) and linolenic (18:3 n-3) acid was observed, resulting in a strong accumulation of 18:1 t11, 18:1t10 and 18:2 t11c15. Denaturing gradient gel electrophoresis (DGGE) was performed to study the rumen bacterial and ciliate population. Algal supplementation provoked changes in both communities. Ciliate DGGE profiles suggested a decreased abundance of Isotricha prostoma and I. intestinalis and some species of Epidinium caudatum, Eudiplodinium maggii and Diplodinium dentaturn in the rumen of algae-fed cows. Moreover, quantitative PCR indicated that incomplete biohydrogenation through algal addition was associated with decreased ciliate numbers. The simultaneous accumulation of hydrogenation intermediates and the disappearance of ciliates suggest that ciliates and/or their associated bacteria could play a role in rumen biohydrogenation.},
  author       = {Boeckaert, Charlotte and Fievez, Veerle and Van Hecke, Dries and Verstraete, Willy and Boon, Nico},
  issn         = {1438-7697},
  journal      = {EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY},
  keyword      = {CONJUGATED LINOLEIC-ACID,qPCR,microalgae,18 : 1 trans fatty acids,protozoa,UNSATURATED FATTY-ACIDS,biohydrogenation,GRADIENT GEL-ELECTROPHORESIS,LINSEED OIL SUPPLEMENTATION,16S RIBOSOMAL-RNA,FED FISH-OIL,LIPID-METABOLISM,STEARIC-ACID,BUTYRIVIBRIO-FIBRISOLVENS,MICROBIAL COMMUNITY},
  language     = {eng},
  number       = {8},
  pages        = {767--777},
  title        = {Changes in rumen biohydrogenation intermediates and ciliate protozoa diversity after algae supplementation to dairy cattle},
  url          = {http://dx.doi.org/10.1002/ejlt.200700052},
  volume       = {109},
  year         = {2007},
}

Chicago
Boeckaert, Charlotte, Veerle Fievez, Dries Van Hecke, Willy Verstraete, and Nico Boon. 2007. “Changes in Rumen Biohydrogenation Intermediates and Ciliate Protozoa Diversity After Algae Supplementation to Dairy Cattle.” European Journal of Lipid Science and Technology 109 (8): 767–777.
APA
Boeckaert, C., Fievez, V., Van Hecke, D., Verstraete, W., & Boon, N. (2007). Changes in rumen biohydrogenation intermediates and ciliate protozoa diversity after algae supplementation to dairy cattle. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, 109(8), 767–777.
Vancouver
1.
Boeckaert C, Fievez V, Van Hecke D, Verstraete W, Boon N. Changes in rumen biohydrogenation intermediates and ciliate protozoa diversity after algae supplementation to dairy cattle. EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY. 2007;109(8):767–77.
MLA
Boeckaert, Charlotte, Veerle Fievez, Dries Van Hecke, et al. “Changes in Rumen Biohydrogenation Intermediates and Ciliate Protozoa Diversity After Algae Supplementation to Dairy Cattle.” EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY 109.8 (2007): 767–777. Print.