Advanced search
1 file | 201.64 KB

Knocking out the MFE-2 gene of Candida bombicola leads to improved medium-chain sophorolipid production

Inge Van Bogaert (UGent) , Julia Sabirova (UGent) , Dirk Develter, Wim Soetaert (UGent) and Erick Vandamme (UGent)
(2009) FEMS YEAST RESEARCH. 9(4). p.610-617
Author
Organization
Abstract
The nonpathogenic yeast Candida bombicola synthesizes sophorolipids. These biosurfactants are composed of the disaccharide sophorose linked to a long-chain hydroxy fatty acid and have potential applications in the food, pharmaceutical, cosmetic and cleaning industries. In order to expand the range of application, a shift of the fatty acid moiety towards medium-chain lengths would be recommendable. However, the synthesis of medium-chain sophorolipids by C. bombicola is a challenging objective. First of all, these sophorolipids can only be obtained by fermentations on unconventional carbon sources, which often have a toxic effect on the cells. Furthermore, medium-chain substrates are partially metabolized in the beta-oxidation pathway. In order to redirect unconventional substrates towards sophorolipid synthesis, the beta-oxidation pathway was blocked on the genome level by knocking out the multifunctional enzyme type 2 (MFE-2) gene. The total gene sequence of the C. bombicola MFE-2 (6033 bp) was cloned (GenBank accession number EU371724), and the obtained nucleotide sequence was used to construct a knock-out cassette. Several knock-out mutants with the correct geno- and phenotype were evaluated in a fermentation on 1-dodecanol. All mutants showed a 1.7-2.9 times higher production of sophorolipids, indicating that in those strains the substrate is redirected towards the sophorolipid synthesis.
Keywords
MFE-2, sophorolipids, Candida bombicola, biosurfactants, yeast, beta-oxidation, YEAST YARROWIA-LIPOLYTICA, COENZYME-A OXIDASE, SACCHAROMYCES-CEREVISIAE, ACYL-COENZYME, MICROBIAL-PRODUCTION, BETA-OXIDATION, SEQUENCE, PROTEIN, CLONING, DEHYDROGENASE

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 201.64 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Van Bogaert, Inge, Julia Sabirova, Dirk Develter, Wim Soetaert, and Erick Vandamme. 2009. “Knocking Out the MFE-2 Gene of Candida Bombicola Leads to Improved Medium-chain Sophorolipid Production.” Fems Yeast Research 9 (4): 610–617.
APA
Van Bogaert, Inge, Sabirova, J., Develter, D., Soetaert, W., & Vandamme, E. (2009). Knocking out the MFE-2 gene of Candida bombicola leads to improved medium-chain sophorolipid production. FEMS YEAST RESEARCH, 9(4), 610–617.
Vancouver
1.
Van Bogaert I, Sabirova J, Develter D, Soetaert W, Vandamme E. Knocking out the MFE-2 gene of Candida bombicola leads to improved medium-chain sophorolipid production. FEMS YEAST RESEARCH. 2009;9(4):610–7.
MLA
Van Bogaert, Inge, Julia Sabirova, Dirk Develter, et al. “Knocking Out the MFE-2 Gene of Candida Bombicola Leads to Improved Medium-chain Sophorolipid Production.” FEMS YEAST RESEARCH 9.4 (2009): 610–617. Print.
@article{524785,
  abstract     = {The nonpathogenic yeast Candida bombicola synthesizes sophorolipids. These biosurfactants are composed of the disaccharide sophorose linked to a long-chain hydroxy fatty acid and have potential applications in the food, pharmaceutical, cosmetic and cleaning industries. In order to expand the range of application, a shift of the fatty acid moiety towards medium-chain lengths would be recommendable. However, the synthesis of medium-chain sophorolipids by C. bombicola is a challenging objective. First of all, these sophorolipids can only be obtained by fermentations on unconventional carbon sources, which often have a toxic effect on the cells. Furthermore, medium-chain substrates are partially metabolized in the beta-oxidation pathway. In order to redirect unconventional substrates towards sophorolipid synthesis, the beta-oxidation pathway was blocked on the genome level by knocking out the multifunctional enzyme type 2 (MFE-2) gene. The total gene sequence of the C. bombicola MFE-2 (6033 bp) was cloned (GenBank accession number EU371724), and the obtained nucleotide sequence was used to construct a knock-out cassette. Several knock-out mutants with the correct geno- and phenotype were evaluated in a fermentation on 1-dodecanol. All mutants showed a 1.7-2.9 times higher production of sophorolipids, indicating that in those strains the substrate is redirected towards the sophorolipid synthesis.},
  author       = {Van Bogaert, Inge and Sabirova, Julia and Develter, Dirk and Soetaert, Wim and Vandamme, Erick},
  issn         = {1567-1356},
  journal      = {FEMS YEAST RESEARCH},
  language     = {eng},
  number       = {4},
  pages        = {610--617},
  title        = {Knocking out the MFE-2 gene of Candida bombicola leads to improved medium-chain sophorolipid production},
  url          = {http://dx.doi.org/10.1111/j.1567-1364.2009.00501.x},
  volume       = {9},
  year         = {2009},
}

Altmetric
View in Altmetric
Web of Science
Times cited: