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Interaction of plasma proteins with commercial protein repellent polyvinyl chloride (PVC): a word of caution

Filip De Somer UGent, Anita Van Landschoot UGent, Guido Van Nooten UGent and Joris De Langhe (2008) PERFUSION-UK. 23(4). p.215-221
abstract
Protein adsorption onto polymers remains a problem. In recent years, several protein-repellent PVC tubings have been developed. Although several studies report the interaction between plasma coagulation proteins and PVC, few address the interaction with other plasma proteins. Two commercial brands of untreated medical grade PVC tubing, phosphorylcholine-coated PVC tubing, triblock-copolymer (polycaprolactone-polydimethylsiloxane-polycaprolactone)-treated PVC tubing and poly-2-methoxyethylacrylate (PMEA)-coated tubing were exposed for 60 minutes to human plasma. A broad spectrum of plasma proteins was found on all tubing. The adsorbed albumin to total protein ratio is lower than the similar ratio in plasma while alpha 1 and alpha 2 globulins are over-represented in the protein spectrum. On PMEA tubing, not only a globulins, but also beta and gamma globulins, are found in high concentrations in the adsorbed protein. PMEA tubing and uncoated PVC tubing of brand B had a higher amount of protein adsorbed compared against all other tubing (p < 0.05). There were no statistical differences in protein adsorption between the triblock-copolymer-treated tubing, the phosphorylcholine-coated tubing and the uncoated PVC tubing of brand A. The average thickness of the protein layer was 23 nm. Plasma protein adsorption still exists on uncoated and protein-repellent tubing and can initiate a systemic inflammatory reaction. Perfusion (2008) 23, 215-221.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
URINARY PROTEIN, SURFACE, COMPLEMENT ACTIVATION, CARDIOPULMONARY BYPASS, protein repellent tubing, HUMAN-SERUM-ALBUMIN, protein adsorption, cardiopulmonary bypass, haemocompatibility, BINDING, HEPARIN, PLASTICIZER, ADSORPTION, BLOOD
journal title
PERFUSION-UK
volume
23
issue
4
pages
215 - 221
publisher
SAGE PUBLICATIONS LTD
place of publication
1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND
Web of Science type
Article
Web of Science id
000262954700004
JCR category
PERIPHERAL VASCULAR DISEASE
JCR impact factor
0.667 (2008)
JCR rank
53/56 (2008)
JCR quartile
4 (2008)
ISSN
0267-6591
DOI
10.1177/0267659108099814
language
English
UGent publication?
yes
classification
A1
id
523088
handle
http://hdl.handle.net/1854/LU-523088
date created
2009-03-18 14:45:37
date last changed
2017-01-02 09:56:41
@article{523088,
  abstract     = {Protein adsorption onto polymers remains a problem. In recent years, several protein-repellent PVC tubings have been developed. Although several studies report the interaction between plasma coagulation proteins and PVC, few address the interaction with other plasma proteins. Two commercial brands of untreated medical grade PVC tubing, phosphorylcholine-coated PVC tubing, triblock-copolymer (polycaprolactone-polydimethylsiloxane-polycaprolactone)-treated PVC tubing and poly-2-methoxyethylacrylate (PMEA)-coated tubing were exposed for 60 minutes to human plasma. A broad spectrum of plasma proteins was found on all tubing. The adsorbed albumin to total protein ratio is lower than the similar ratio in plasma while alpha 1 and alpha 2 globulins are over-represented in the protein spectrum. On PMEA tubing, not only a globulins, but also beta and gamma globulins, are found in high concentrations in the adsorbed protein. PMEA tubing and uncoated PVC tubing of brand B had a higher amount of protein adsorbed compared against all other tubing (p {\textlangle} 0.05). There were no statistical differences in protein adsorption between the triblock-copolymer-treated tubing, the phosphorylcholine-coated tubing and the uncoated PVC tubing of brand A. The average thickness of the protein layer was 23 nm. Plasma protein adsorption still exists on uncoated and protein-repellent tubing and can initiate a systemic inflammatory reaction. Perfusion (2008) 23, 215-221.},
  author       = {De Somer, Filip and Van Landschoot, Anita and Van Nooten, Guido and De Langhe, Joris},
  issn         = {0267-6591},
  journal      = {PERFUSION-UK},
  keyword      = {URINARY PROTEIN,SURFACE,COMPLEMENT ACTIVATION,CARDIOPULMONARY BYPASS,protein repellent tubing,HUMAN-SERUM-ALBUMIN,protein adsorption,cardiopulmonary bypass,haemocompatibility,BINDING,HEPARIN,PLASTICIZER,ADSORPTION,BLOOD},
  language     = {eng},
  number       = {4},
  pages        = {215--221},
  publisher    = {SAGE PUBLICATIONS LTD},
  title        = {Interaction of plasma proteins with commercial protein repellent polyvinyl chloride (PVC): a word of caution},
  url          = {http://dx.doi.org/10.1177/0267659108099814},
  volume       = {23},
  year         = {2008},
}

Chicago
De Somer, Filip, Anita Van Landschoot, Guido Van Nooten, and Joris De Langhe. 2008. “Interaction of Plasma Proteins with Commercial Protein Repellent Polyvinyl Chloride (PVC): a Word of Caution.” Perfusion-uk 23 (4): 215–221.
APA
De Somer, F., Van Landschoot, A., Van Nooten, G., & De Langhe, J. (2008). Interaction of plasma proteins with commercial protein repellent polyvinyl chloride (PVC): a word of caution. PERFUSION-UK, 23(4), 215–221.
Vancouver
1.
De Somer F, Van Landschoot A, Van Nooten G, De Langhe J. Interaction of plasma proteins with commercial protein repellent polyvinyl chloride (PVC): a word of caution. PERFUSION-UK. 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND: SAGE PUBLICATIONS LTD; 2008;23(4):215–21.
MLA
De Somer, Filip, Anita Van Landschoot, Guido Van Nooten, et al. “Interaction of Plasma Proteins with Commercial Protein Repellent Polyvinyl Chloride (PVC): a Word of Caution.” PERFUSION-UK 23.4 (2008): 215–221. Print.