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Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients

(2009) JOURNAL OF PROTEOME RESEARCH. 8(1 (Sp. Iss.)). p.335-345
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Abstract
Coronary artery disease (CAD) is a major cause of mortality and morbidity. Noninvasive proteome analysis could guide clinical evaluation and early/preventive treatment. Under routine clinical conditions, urine of 67 patients presenting with symptoms suspicious for CAD were analyzed by capillary electrophoresis directly coupled with mass spectrometry (CE-MS). All patients were subjected to coronary angiography and either assigned to a CAD or non-CAD group. A training set of 29 patients was used to establish CAD and non-CAD-associated proteome patterns of plasma as well as urine. Significant discriminatory power was achieved in urine but not in plasma. Therefore, urine proteomic analysis of further 38 patients was performed in a blinded study. A combination of 17 urinary polypeptides allowed separation of both groups in the test set with a sensitivity of 81 %, a specificity of 92%, and an accuracy of 84%. Sequencing of urinary marker peptides identified fragments of collagen alpha 1 (I and III), which we furthermore demonstrated to be expressed in atherosclerotic plaques of human aorta. In conclusion, specific CE-MS polypeptide patterns in urine were associated with significant CAD in patients with angina-typical symptoms. These promising findings need to be further evaluated in regard to reliability of a urine-based screening method with the potential of improving the diagnostic approaches for CAD.
Keywords
INFLAMMATION, SEPARATION, CARDIOVASCULAR-DISEASE, BIOMARKER DISCOVERY, CAPILLARY-ELECTROPHORESIS, CHRONIC KIDNEY-DISEASE, MASS-SPECTROMETRY, PEPTIDES, SYSTEM, CANCER

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MLA
von zur Muhlen, Constantin, Eric Schiffer, Petra Zuerbig, et al. “Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients.” JOURNAL OF PROTEOME RESEARCH 8.1 (Sp. Iss.) (2009): 335–345. Print.
APA
von zur Muhlen, C., Schiffer, E., Zuerbig, P., Kellmann, M., Brasse, M., Meert, N., Vanholder, R., et al. (2009). Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients. JOURNAL OF PROTEOME RESEARCH, 8(1 (Sp. Iss.)), 335–345.
Chicago author-date
von zur Muhlen, Constantin, Eric Schiffer, Petra Zuerbig, Marcus Kellmann, Mario Brasse, Natalie Meert, Raymond Vanholder, et al. 2009. “Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients.” Journal of Proteome Research 8 (1 (Sp. Iss.)): 335–345.
Chicago author-date (all authors)
von zur Muhlen, Constantin, Eric Schiffer, Petra Zuerbig, Marcus Kellmann, Mario Brasse, Natalie Meert, Raymond Vanholder, Anna Dominiczak, Yung Chih Chen, Harald Mischak, Christoph Bode, and Karlheinz Peter. 2009. “Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients.” Journal of Proteome Research 8 (1 (Sp. Iss.)): 335–345.
Vancouver
1.
von zur Muhlen C, Schiffer E, Zuerbig P, Kellmann M, Brasse M, Meert N, et al. Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients. JOURNAL OF PROTEOME RESEARCH. 2009;8(1 (Sp. Iss.)):335–45.
IEEE
[1]
C. von zur Muhlen et al., “Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients,” JOURNAL OF PROTEOME RESEARCH, vol. 8, no. 1 (Sp. Iss.), pp. 335–345, 2009.
@article{519890,
  abstract     = {Coronary artery disease (CAD) is a major cause of mortality and morbidity. Noninvasive proteome analysis could guide clinical evaluation and early/preventive treatment. Under routine clinical conditions, urine of 67 patients presenting with symptoms suspicious for CAD were analyzed by capillary electrophoresis directly coupled with mass spectrometry (CE-MS). All patients were subjected to coronary angiography and either assigned to a CAD or non-CAD group. A training set of 29 patients was used to establish CAD and non-CAD-associated proteome patterns of plasma as well as urine. Significant discriminatory power was achieved in urine but not in plasma. Therefore, urine proteomic analysis of further 38 patients was performed in a blinded study. A combination of 17 urinary polypeptides allowed separation of both groups in the test set with a sensitivity of 81 %, a specificity of 92%, and an accuracy of 84%. Sequencing of urinary marker peptides identified fragments of collagen alpha 1 (I and III), which we furthermore demonstrated to be expressed in atherosclerotic plaques of human aorta. In conclusion, specific CE-MS polypeptide patterns in urine were associated with significant CAD in patients with angina-typical symptoms. These promising findings need to be further evaluated in regard to reliability of a urine-based screening method with the potential of improving the diagnostic approaches for CAD.},
  author       = {von zur Muhlen, Constantin and Schiffer, Eric and Zuerbig, Petra and Kellmann, Marcus and Brasse, Mario and Meert, Natalie and Vanholder, Raymond and Dominiczak, Anna and Chen, Yung Chih and Mischak, Harald and Bode, Christoph and Peter, Karlheinz},
  issn         = {1535-3893},
  journal      = {JOURNAL OF PROTEOME RESEARCH},
  keywords     = {INFLAMMATION,SEPARATION,CARDIOVASCULAR-DISEASE,BIOMARKER DISCOVERY,CAPILLARY-ELECTROPHORESIS,CHRONIC KIDNEY-DISEASE,MASS-SPECTROMETRY,PEPTIDES,SYSTEM,CANCER},
  language     = {eng},
  number       = {1 (Sp. Iss.)},
  pages        = {335--345},
  title        = {Evaluation of Urine Proteome Pattern Analysis for Its Potential To Reflect Coronary Artery Atherosclerosis in Symptomatic Patients},
  url          = {http://dx.doi.org/10.1021/pr800615t},
  volume       = {8},
  year         = {2009},
}

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