Standardization of real-time PCR gene expression data from independent biological replicates
- Author
- Erik Willems, Luc Leyns and Jo Vandesompele (UGent)
- Organization
- Abstract
- Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Corrective methods for different steps in the qRT-PCR reaction have been reported; however, statistical analysis and presentation of substantially variable biological repeats present problems and are often not meaningful, for example, in a biological system such as mouse embryonic stem cell differentiation. Based on a series of sequential corrections, including log transformation, mean centering, and autoscaling, we describe a robust and powerful standardization method that can be used on highly variable data sets to draw statistically reliable conclusions.
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 368.91 KB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-518134
- MLA
- Willems, Erik, et al. “Standardization of Real-Time PCR Gene Expression Data from Independent Biological Replicates.” ANALYTICAL BIOCHEMISTRY, vol. 379, no. 1, ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, 2008, pp. 127–29, doi:10.1016/j.ab.2008.04.036.
- APA
- Willems, E., Leyns, L., & Vandesompele, J. (2008). Standardization of real-time PCR gene expression data from independent biological replicates. https://doi.org/10.1016/j.ab.2008.04.036
- Chicago author-date
- Willems, Erik, Luc Leyns, and Jo Vandesompele. 2008. “Standardization of Real-Time PCR Gene Expression Data from Independent Biological Replicates.” ANALYTICAL BIOCHEMISTRY. SAN DIEGO, CA 92101-4495 USA: ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900,. https://doi.org/10.1016/j.ab.2008.04.036.
- Chicago author-date (all authors)
- Willems, Erik, Luc Leyns, and Jo Vandesompele. 2008. “Standardization of Real-Time PCR Gene Expression Data from Independent Biological Replicates.” ANALYTICAL BIOCHEMISTRY. SAN DIEGO, CA 92101-4495 USA: ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900,. doi:10.1016/j.ab.2008.04.036.
- Vancouver
- 1.Willems E, Leyns L, Vandesompele J. Standardization of real-time PCR gene expression data from independent biological replicates. Vol. 379, ANALYTICAL BIOCHEMISTRY. SAN DIEGO, CA 92101-4495 USA: ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900,; 2008. p. 127–9.
- IEEE
- [1]E. Willems, L. Leyns, and J. Vandesompele, “Standardization of real-time PCR gene expression data from independent biological replicates,” ANALYTICAL BIOCHEMISTRY, vol. 379, no. 1. ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA, pp. 127–129, 2008.
@misc{518134,
abstract = {{Gene expression analysis by quantitative reverse transcription PCR (qRT-PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Corrective methods for different steps in the qRT-PCR reaction have been reported; however, statistical analysis and presentation of substantially variable biological repeats present problems and are often not meaningful, for example, in a biological system such as mouse embryonic stem cell differentiation. Based on a series of sequential corrections, including log transformation, mean centering, and autoscaling, we describe a robust and powerful standardization method that can be used on highly variable data sets to draw statistically reliable conclusions.}},
author = {{Willems, Erik and Leyns, Luc and Vandesompele, Jo}},
issn = {{0003-2697}},
language = {{eng}},
number = {{1}},
pages = {{127--129}},
publisher = {{ACADEMIC PRESS INC ELSEVIER SCIENCE, 525 B ST, STE 1900,}},
series = {{ANALYTICAL BIOCHEMISTRY}},
title = {{Standardization of real-time PCR gene expression data from independent biological replicates}},
url = {{http://doi.org/10.1016/j.ab.2008.04.036}},
volume = {{379}},
year = {{2008}},
}
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: