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Effect of ionomycin on oocyte activation and embryo development in mouse

Elke Heytens UGent, Reza Soleimani UGent, Sylvie Lierman UGent, Simon De Meester, Jan Gerris UGent, MARC DHONT UGent, Josiane Van der Elst UGent and Petra De Sutter UGent (2008) REPRODUCTIVE BIOMEDICINE ONLINE. 17(6). p.764-771
abstract
Artificial oocyte activation using the calcium ionophore ionomycin is applied successfully in assisted reproduction but some concern exists oil the clinical use. The aims of the present study were to optimize the oocyte activation scheme and to address embryo toxicity in a mouse model. Efficiency of oocyte activation and subsequent development was evaluated and ionomycin was found to be an efficient activator at 10 mu mol/l. An improved effect of a second exposure to 5 pmol/l ionomycin oil blastocyst development was observed. Toxicity of ionomycin on embryos was then investigated by evaluating pre- and post-implantation development of in-vivo fertilized oocytes following exposure to ionomycin. Blastocyst development, blastocyst cell numbers in trophectoderm and inner cell mass were not different between treated and non-treated zygotes. Also implantation rates and fetal parameters such as length, weight and morphological parameters were similar between the fetuses originating from zygotes treated with ionomycin and non-treated zygotes. Furthermore, healthy offspring originating from ionomycin-treated zygotes was born. In conclusion, no adverse effects of ionomycin on in-vitro or in-vivo mouse embryo development were noticed, giving arguments in favour of the use of ionomycin, although negative long-term effects of this compound cannot be excluded at present.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
REPRODUCTIVE BIOMEDICINE ONLINE
Reprod. Biomed. Online
volume
17
issue
6
pages
764 - 771
Web of Science type
Article
Web of Science id
000261903400005
JCR category
OBSTETRICS & GYNECOLOGY
JCR impact factor
2.954 (2008)
JCR rank
11/61 (2008)
JCR quartile
1 (2008)
ISSN
1472-6483
language
English
UGent publication?
yes
classification
A1
id
516200
handle
http://hdl.handle.net/1854/LU-516200
date created
2009-03-10 09:23:41
date last changed
2009-03-16 08:44:13
@article{516200,
  abstract     = {Artificial oocyte activation using the calcium ionophore ionomycin is applied successfully in assisted reproduction but some concern exists oil the clinical use. The aims of the present study were to optimize the oocyte activation scheme and to address embryo toxicity in a mouse model. Efficiency of oocyte activation and subsequent development was evaluated and ionomycin was found to be an efficient activator at 10 mu mol/l. An improved effect of a second exposure to 5 pmol/l ionomycin oil blastocyst development was observed. Toxicity of ionomycin on embryos was then investigated by evaluating pre- and post-implantation development of in-vivo fertilized oocytes following exposure to ionomycin. Blastocyst development, blastocyst cell numbers in trophectoderm and inner cell mass were not different between treated and non-treated zygotes. Also implantation rates and fetal parameters such as length, weight and morphological parameters were similar between the fetuses originating from zygotes treated with ionomycin and non-treated zygotes. Furthermore, healthy offspring originating from ionomycin-treated zygotes was born. In conclusion, no adverse effects of ionomycin on in-vitro or in-vivo mouse embryo development were noticed, giving arguments in favour of the use of ionomycin, although negative long-term effects of this compound cannot be excluded at present.},
  author       = {Heytens, Elke and Soleimani, Reza and Lierman, Sylvie and De Meester, Simon and Gerris, Jan and DHONT, MARC and Van der Elst, Josiane and De Sutter, Petra},
  issn         = {1472-6483},
  journal      = {REPRODUCTIVE BIOMEDICINE ONLINE},
  language     = {eng},
  number       = {6},
  pages        = {764--771},
  title        = {Effect of ionomycin on oocyte activation and embryo development in mouse},
  volume       = {17},
  year         = {2008},
}

Chicago
Heytens, Elke, Reza Soleimani, Sylvie Lierman, Simon De Meester, Jan Gerris, MARC DHONT, Josiane Van der Elst, and Petra De Sutter. 2008. “Effect of Ionomycin on Oocyte Activation and Embryo Development in Mouse.” Reproductive Biomedicine Online 17 (6): 764–771.
APA
Heytens, E., Soleimani, R., Lierman, S., De Meester, S., Gerris, J., DHONT, M., Van der Elst, J., et al. (2008). Effect of ionomycin on oocyte activation and embryo development in mouse. REPRODUCTIVE BIOMEDICINE ONLINE, 17(6), 764–771.
Vancouver
1.
Heytens E, Soleimani R, Lierman S, De Meester S, Gerris J, DHONT M, et al. Effect of ionomycin on oocyte activation and embryo development in mouse. REPRODUCTIVE BIOMEDICINE ONLINE. 2008;17(6):764–71.
MLA
Heytens, Elke, Reza Soleimani, Sylvie Lierman, et al. “Effect of Ionomycin on Oocyte Activation and Embryo Development in Mouse.” REPRODUCTIVE BIOMEDICINE ONLINE 17.6 (2008): 764–771. Print.