Advanced search
Add to list

Effect of ionomycin on oocyte activation and embryo development in mouse

Author
Organization
Abstract
Artificial oocyte activation using the calcium ionophore ionomycin is applied successfully in assisted reproduction but some concern exists oil the clinical use. The aims of the present study were to optimize the oocyte activation scheme and to address embryo toxicity in a mouse model. Efficiency of oocyte activation and subsequent development was evaluated and ionomycin was found to be an efficient activator at 10 mu mol/l. An improved effect of a second exposure to 5 pmol/l ionomycin oil blastocyst development was observed. Toxicity of ionomycin on embryos was then investigated by evaluating pre- and post-implantation development of in-vivo fertilized oocytes following exposure to ionomycin. Blastocyst development, blastocyst cell numbers in trophectoderm and inner cell mass were not different between treated and non-treated zygotes. Also implantation rates and fetal parameters such as length, weight and morphological parameters were similar between the fetuses originating from zygotes treated with ionomycin and non-treated zygotes. Furthermore, healthy offspring originating from ionomycin-treated zygotes was born. In conclusion, no adverse effects of ionomycin on in-vitro or in-vivo mouse embryo development were noticed, giving arguments in favour of the use of ionomycin, although negative long-term effects of this compound cannot be excluded at present.

Citation

Please use this url to cite or link to this publication:

MLA
Heytens, Elke, et al. “Effect of Ionomycin on Oocyte Activation and Embryo Development in Mouse.” REPRODUCTIVE BIOMEDICINE ONLINE, vol. 17, no. 6, 2008, pp. 764–71.
APA
Heytens, E., Soleimani, R., Lierman, S., De Meester, S., Gerris, J., Dhont, M., … De Sutter, P. (2008). Effect of ionomycin on oocyte activation and embryo development in mouse. REPRODUCTIVE BIOMEDICINE ONLINE, 17(6), 764–771.
Chicago author-date
Heytens, Elke, Reza Soleimani, Sylvie Lierman, Simon De Meester, Jan Gerris, Marc Dhont, Josiane Van der Elst, and Petra De Sutter. 2008. “Effect of Ionomycin on Oocyte Activation and Embryo Development in Mouse.” REPRODUCTIVE BIOMEDICINE ONLINE 17 (6): 764–71.
Chicago author-date (all authors)
Heytens, Elke, Reza Soleimani, Sylvie Lierman, Simon De Meester, Jan Gerris, Marc Dhont, Josiane Van der Elst, and Petra De Sutter. 2008. “Effect of Ionomycin on Oocyte Activation and Embryo Development in Mouse.” REPRODUCTIVE BIOMEDICINE ONLINE 17 (6): 764–771.
Vancouver
1.
Heytens E, Soleimani R, Lierman S, De Meester S, Gerris J, Dhont M, et al. Effect of ionomycin on oocyte activation and embryo development in mouse. REPRODUCTIVE BIOMEDICINE ONLINE. 2008;17(6):764–71.
IEEE
[1]
E. Heytens et al., “Effect of ionomycin on oocyte activation and embryo development in mouse,” REPRODUCTIVE BIOMEDICINE ONLINE, vol. 17, no. 6, pp. 764–771, 2008.
@article{516200,
  abstract     = {{Artificial oocyte activation using the calcium ionophore ionomycin is applied successfully in assisted reproduction but some concern exists oil the clinical use. The aims of the present study were to optimize the oocyte activation scheme and to address embryo toxicity in a mouse model. Efficiency of oocyte activation and subsequent development was evaluated and ionomycin was found to be an efficient activator at 10 mu mol/l. An improved effect of a second exposure to 5 pmol/l ionomycin oil blastocyst development was observed. Toxicity of ionomycin on embryos was then investigated by evaluating pre- and post-implantation development of in-vivo fertilized oocytes following exposure to ionomycin. Blastocyst development, blastocyst cell numbers in trophectoderm and inner cell mass were not different between treated and non-treated zygotes. Also implantation rates and fetal parameters such as length, weight and morphological parameters were similar between the fetuses originating from zygotes treated with ionomycin and non-treated zygotes. Furthermore, healthy offspring originating from ionomycin-treated zygotes was born. In conclusion, no adverse effects of ionomycin on in-vitro or in-vivo mouse embryo development were noticed, giving arguments in favour of the use of ionomycin, although negative long-term effects of this compound cannot be excluded at present.}},
  author       = {{Heytens, Elke and Soleimani, Reza and Lierman, Sylvie and De Meester, Simon and Gerris, Jan and Dhont, Marc and Van der Elst, Josiane and De Sutter, Petra}},
  issn         = {{1472-6483}},
  journal      = {{REPRODUCTIVE BIOMEDICINE ONLINE}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{764--771}},
  title        = {{Effect of ionomycin on oocyte activation and embryo development in mouse}},
  volume       = {{17}},
  year         = {{2008}},
}

Web of Science
Times cited: