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Aberrant methylation of candidate tumor suppressor genes in neuroblastoma

(2009) CANCER LETTERS. 273(2). p.336-346
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Abstract
CpG island hypermethylation has been recognized as an alternative mechanism for tumor suppressor gene inactivation. In this study, we performed methylation-specific PCR (MSP) to investigate the methylation status of 10 selected tumor suppressor genes in neuroblastoma. Seven of the investigated genes (CD44, RASSF1A, CASP8, PTEN, ZMYND10, CDH1, PRDM2) showed high frequencies (>= 30%) of methylation in 33 neuroblastoma cell lines. In 42 primary neuroblastoma tumors, the frequencies of methylation were 69%, CD44; 71%, RASSF1A; 56%, CASP8; 25%, PTEN; 15%, ZMYND10; 8%, CDH1; and 0%, PRDM2. Furthermore, CASP8 and CDH1 hypermethylation was significantly associated with poor event-free survival. Meta-analysis of 115 neuroblastoma tumors demonstrated a significant correlation between CASP8 methylation and MYCN amplification. In addition, there was a correlation between ZMYND10 methylation and MYCN amplification. The MSP data, together with optimized mRNA re-expression experiments (in terms of concentration and time of treatment and use of proper reference genes) further strengthen the notion that epigenetic alterations could play a significant role in NB oncogenesis. This study thus warrants the need for a global profiling of gene promoter hypermethylation to identify genome-wide aberrantly methylated genes in order to further understand neuroblastoma pathogenesis and to identify prognostic methylation markers.

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MLA
Hoebeeck, Jasmien, et al. “Aberrant Methylation of Candidate Tumor Suppressor Genes in Neuroblastoma.” CANCER LETTERS, vol. 273, no. 2, 2009, pp. 336–46, doi:10.1016/j.canlet.2008.08.019.
APA
Hoebeeck, J., Michels, E., Pattyn, F., Combaret, V., Vermeulen, J., Yigit, N., … Vandesompele, J. (2009). Aberrant methylation of candidate tumor suppressor genes in neuroblastoma. CANCER LETTERS, 273(2), 336–346. https://doi.org/10.1016/j.canlet.2008.08.019
Chicago author-date
Hoebeeck, Jasmien, Evi Michels, Filip Pattyn, Valerie Combaret, Joelle Vermeulen, Nurten Yigit, Claire Hoyoux, et al. 2009. “Aberrant Methylation of Candidate Tumor Suppressor Genes in Neuroblastoma.” CANCER LETTERS 273 (2): 336–46. https://doi.org/10.1016/j.canlet.2008.08.019.
Chicago author-date (all authors)
Hoebeeck, Jasmien, Evi Michels, Filip Pattyn, Valerie Combaret, Joelle Vermeulen, Nurten Yigit, Claire Hoyoux, Genevieve Laureys, Anne De Paepe, Franki Speleman, and Jo Vandesompele. 2009. “Aberrant Methylation of Candidate Tumor Suppressor Genes in Neuroblastoma.” CANCER LETTERS 273 (2): 336–346. doi:10.1016/j.canlet.2008.08.019.
Vancouver
1.
Hoebeeck J, Michels E, Pattyn F, Combaret V, Vermeulen J, Yigit N, et al. Aberrant methylation of candidate tumor suppressor genes in neuroblastoma. CANCER LETTERS. 2009;273(2):336–46.
IEEE
[1]
J. Hoebeeck et al., “Aberrant methylation of candidate tumor suppressor genes in neuroblastoma,” CANCER LETTERS, vol. 273, no. 2, pp. 336–346, 2009.
@article{516097,
  abstract     = {{CpG island hypermethylation has been recognized as an alternative mechanism for tumor suppressor gene inactivation. In this study, we performed methylation-specific PCR (MSP) to investigate the methylation status of 10 selected tumor suppressor genes in neuroblastoma. Seven of the investigated genes (CD44, RASSF1A, CASP8, PTEN, ZMYND10, CDH1, PRDM2) showed high frequencies (>= 30%) of methylation in 33 neuroblastoma cell lines. In 42 primary neuroblastoma tumors, the frequencies of methylation were 69%, CD44; 71%, RASSF1A; 56%, CASP8; 25%, PTEN; 15%, ZMYND10; 8%, CDH1; and 0%, PRDM2. Furthermore, CASP8 and CDH1 hypermethylation was significantly associated with poor event-free survival. Meta-analysis of 115 neuroblastoma tumors demonstrated a significant correlation between CASP8 methylation and MYCN amplification. In addition, there was a correlation between ZMYND10 methylation and MYCN amplification. The MSP data, together with optimized mRNA re-expression experiments (in terms of concentration and time of treatment and use of proper reference genes) further strengthen the notion that epigenetic alterations could play a significant role in NB oncogenesis. This study thus warrants the need for a global profiling of gene promoter hypermethylation to identify genome-wide aberrantly methylated genes in order to further understand neuroblastoma pathogenesis and to identify prognostic methylation markers.}},
  author       = {{Hoebeeck, Jasmien and Michels, Evi and Pattyn, Filip and Combaret, Valerie and Vermeulen, Joelle and Yigit, Nurten and Hoyoux, Claire and Laureys, Genevieve and De Paepe, Anne and Speleman, Franki and Vandesompele, Jo}},
  issn         = {{0304-3835}},
  journal      = {{CANCER LETTERS}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{336--346}},
  title        = {{Aberrant methylation of candidate tumor suppressor genes in neuroblastoma}},
  url          = {{http://doi.org/10.1016/j.canlet.2008.08.019}},
  volume       = {{273}},
  year         = {{2009}},
}

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