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New strategies for nucleic acid delivery to conquer cellular and nuclear membranes

Ine Lentacker (UGent) , Roosmarijn Vandenbroucke (UGent) , Bart Lucas (UGent) , Jo Demeester (UGent) , Stefaan De Smedt (UGent) and Niek Sanders (UGent)
(2008) Journal of Controlled Release. 132(3). p.279-288
Author
Organization
Abstract
After administration to the body, nucleic acid containing nanoparticles (NANs) need to cross several extra- and intracellular barriers to reach the cytoplasm or nucleus of the target cells. In the last decade several groups tried to overcome these barriers by arming non-viral delivery systems with targeting moieties, polyethylene glycol chains, fusogenic peptides and so forth. However, the drawback of this upgrading strategy is that each of the encountered barriers requires a new functionality, leading to very complex multicomponent NANs. Moreover, there are currently no components available that can efficiently transport genes or NANs inside the nucleus of non-dividing cells. In this article a new, ultrasound based delivery system that possesses the capacity to simultaneously overcome several key barriers in non-viral nucleic acid delivery is presented. Additionally, a small amphiphilic compound that induces nuclear uptake of plasmid DNA and enhances non-viral gene transfer is presented.
Keywords
Microbubbles, Ultrasound, Liposomes, Nuclear entry, ULTRASOUND-MEDIATED TRANSFECTION, PLASMID-LIPID PARTICLES, GENE-THERAPY PROGRESS, INTRACELLULAR DELIVERY, Gene delivery, LOADED MICROBUBBLES, ALBUMIN MICROBUBBLES, CATIONIC LIPOSOMES, SYSTEMIC DELIVERY, IN-VITRO, DNA

Citation

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Chicago
Lentacker, Ine, Roosmarijn Vandenbroucke, Bart Lucas, Jo Demeester, Stefaan De Smedt, and Niek Sanders. 2008. “New Strategies for Nucleic Acid Delivery to Conquer Cellular and Nuclear Membranes.” Ed. J Feijen, WE Hennink, and AP Sam. Journal of Controlled Release 132 (3): 279–288.
APA
Lentacker, I., Vandenbroucke, R., Lucas, B., Demeester, J., De Smedt, S., & Sanders, N. (2008). New strategies for nucleic acid delivery to conquer cellular and nuclear membranes. (J. Feijen, W. Hennink, & A. Sam, Eds.)Journal of Controlled Release, 132(3), 279–288. Presented at the Tenth European Symposium on Controlled Drug Delivery.
Vancouver
1.
Lentacker I, Vandenbroucke R, Lucas B, Demeester J, De Smedt S, Sanders N. New strategies for nucleic acid delivery to conquer cellular and nuclear membranes. Feijen J, Hennink W, Sam A, editors. Journal of Controlled Release. Amsterdam: Elsevier; 2008;132(3):279–88.
MLA
Lentacker, Ine, Roosmarijn Vandenbroucke, Bart Lucas, et al. “New Strategies for Nucleic Acid Delivery to Conquer Cellular and Nuclear Membranes.” Ed. J Feijen, WE Hennink, & AP Sam. Journal of Controlled Release 132.3 (2008): 279–288. Print.
@article{514114,
  abstract     = {After administration to the body, nucleic acid containing nanoparticles (NANs) need to cross several extra- and intracellular barriers to reach the cytoplasm or nucleus of the target cells. In the last decade several groups tried to overcome these barriers by arming non-viral delivery systems with targeting moieties, polyethylene glycol chains, fusogenic peptides and so forth. However, the drawback of this upgrading strategy is that each of the encountered barriers requires a new functionality, leading to very complex multicomponent NANs. Moreover, there are currently no components available that can efficiently transport genes or NANs inside the nucleus of non-dividing cells. In this article a new, ultrasound based delivery system that possesses the capacity to simultaneously overcome several key barriers in non-viral nucleic acid delivery is presented. Additionally, a small amphiphilic compound that induces nuclear uptake of plasmid DNA and enhances non-viral gene transfer is presented.},
  author       = {Lentacker, Ine and Vandenbroucke, Roosmarijn and Lucas, Bart and Demeester, Jo and De Smedt, Stefaan and Sanders, Niek},
  editor       = {Feijen, J and Hennink, WE and Sam, AP},
  issn         = {0168-3659},
  journal      = {Journal of Controlled Release},
  language     = {eng},
  location     = {Noordwijk aan Zee},
  number       = {3},
  pages        = {279--288},
  publisher    = {Elsevier},
  title        = {New strategies for nucleic acid delivery to conquer cellular and nuclear membranes},
  url          = {http://dx.doi.org/10.1016/j.jconrel.2008.06.023},
  volume       = {132},
  year         = {2008},
}

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