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Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice

(2008) JOURNAL OF CHROMATOGRAPHY A. 1215(1-2). p.125-132
Author
Organization
Abstract
An LC-MS/MS method has been developed and validated for the analysis of six monoglutamate folates in rice. Accurate determination of folates in rice seeds required the optimisation of an extraction procedure encompassing homogenisation, heat treatment, tri-enzyme treatment (alpha-amylase, protease and deconjugase), centrifugation and Ultrafiltration. The resulting extract was loaded onto a Polaris C 18-A column, followed by gradient elution and detection by tandem mass spectrometry. The validated method is sensitive, reproducible, accurate, and linear between 1 and 400 mu g/100 g rice (dry weight). The limit of detection and limit Of quantification in diluted rice matrix ranged between 0.2 and 1.2 mu g/100 g and between 0.6 and 4 mu g/100 g, respectively, depending on the Compound. Intra- and inter-day precision varied, respectively, from 1.0 to 9.1% RSD, and from 3.4 to 13.5% RSD, except for one Compound (19.1% at low concern rations). The accuracy ranged from 96.9 to 115.4%. Matrix effects were compensated for by use of isotopically labelled internal standards. Most folates in rice extracts were stable when kept for 2 weeks at -20 and -80 degrees C, but degraded after three fireeze-thaw cycles or at 4 degrees C, even when stored protected from light. Our validated method offers a sensitive and versatile approach to determine the level of six different monoglutamate folates in a complex matrix as rice.
Keywords
LC-MS/MS, Folates, Tri-enzyme treatment, Rice analysis

Citation

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MLA
De Brouwer, Veerle, et al. “Optimisation and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for Folates in Rice.” JOURNAL OF CHROMATOGRAPHY A, vol. 1215, no. 1–2, Elsevier Science, 2008, pp. 125–32, doi:10.1016/j.chroma.2008.11.004.
APA
De Brouwer, V., Storojenko, S., Van De Steene, J., Wille, S., Stove, C., Van Der Straeten, D., & Lambert, W. (2008). Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice. JOURNAL OF CHROMATOGRAPHY A, 1215(1–2), 125–132. https://doi.org/10.1016/j.chroma.2008.11.004
Chicago author-date
De Brouwer, Veerle, Serguei Storojenko, Jet Van De Steene, Sarah Wille, Christophe Stove, Dominique Van Der Straeten, and Willy Lambert. 2008. “Optimisation and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for Folates in Rice.” JOURNAL OF CHROMATOGRAPHY A 1215 (1–2): 125–32. https://doi.org/10.1016/j.chroma.2008.11.004.
Chicago author-date (all authors)
De Brouwer, Veerle, Serguei Storojenko, Jet Van De Steene, Sarah Wille, Christophe Stove, Dominique Van Der Straeten, and Willy Lambert. 2008. “Optimisation and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method for Folates in Rice.” JOURNAL OF CHROMATOGRAPHY A 1215 (1–2): 125–132. doi:10.1016/j.chroma.2008.11.004.
Vancouver
1.
De Brouwer V, Storojenko S, Van De Steene J, Wille S, Stove C, Van Der Straeten D, et al. Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice. JOURNAL OF CHROMATOGRAPHY A. 2008;1215(1–2):125–32.
IEEE
[1]
V. De Brouwer et al., “Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice,” JOURNAL OF CHROMATOGRAPHY A, vol. 1215, no. 1–2, pp. 125–132, 2008.
@article{513426,
  abstract     = {{An LC-MS/MS method has been developed and validated for the analysis of six monoglutamate folates in rice. Accurate determination of folates in rice seeds required the optimisation of an extraction procedure encompassing homogenisation, heat treatment, tri-enzyme treatment (alpha-amylase, protease and deconjugase), centrifugation and Ultrafiltration. The resulting extract was loaded onto a Polaris C 18-A column, followed by gradient elution and detection by tandem mass spectrometry. The validated method is sensitive, reproducible, accurate, and linear between 1 and 400 mu g/100 g rice (dry weight). The limit of detection and limit Of quantification in diluted rice matrix ranged between 0.2 and 1.2 mu g/100 g and between 0.6 and 4 mu g/100 g, respectively, depending on the Compound. Intra- and inter-day precision varied, respectively, from 1.0 to 9.1% RSD, and from 3.4 to 13.5% RSD, except for one Compound (19.1% at low concern rations). The accuracy ranged from 96.9 to 115.4%. Matrix effects were compensated for by use of isotopically labelled internal standards. Most folates in rice extracts were stable when kept for 2 weeks at -20 and -80 degrees C, but degraded after three fireeze-thaw cycles or at 4 degrees C, even when stored protected from light. Our validated method offers a sensitive and versatile approach to determine the level of six different monoglutamate folates in a complex matrix as rice.}},
  author       = {{De Brouwer, Veerle and Storojenko, Serguei and Van De Steene, Jet and Wille, Sarah and Stove, Christophe and Van Der Straeten, Dominique and Lambert, Willy}},
  issn         = {{0021-9673}},
  journal      = {{JOURNAL OF CHROMATOGRAPHY A}},
  keywords     = {{LC-MS/MS,Folates,Tri-enzyme treatment,Rice analysis}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{125--132}},
  publisher    = {{Elsevier Science}},
  title        = {{Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice}},
  url          = {{http://doi.org/10.1016/j.chroma.2008.11.004}},
  volume       = {{1215}},
  year         = {{2008}},
}

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