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Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice

Veerle De Brouwer UGent, Serguei Storojenko UGent, Jet Van De Steene UGent, Sarah Wille, Dominique Van Der Straeten UGent and Willy Lambert UGent (2008) JOURNAL OF CHROMATOGRAPHY A. 1215(1-2). p.125-132
abstract
An LC-MS/MS method has been developed and validated for the analysis of six monoglutamate folates in rice. Accurate determination of folates in rice seeds required the optimisation of an extraction procedure encompassing homogenisation, heat treatment, tri-enzyme treatment (alpha-amylase, protease and deconjugase), centrifugation and Ultrafiltration. The resulting extract was loaded onto a Polaris C 18-A column, followed by gradient elution and detection by tandem mass spectrometry. The validated method is sensitive, reproducible, accurate, and linear between 1 and 400 mu g/100 g rice (dry weight). The limit of detection and limit Of quantification in diluted rice matrix ranged between 0.2 and 1.2 mu g/100 g and between 0.6 and 4 mu g/100 g, respectively, depending on the Compound. Intra- and inter-day precision varied, respectively, from 1.0 to 9.1% RSD, and from 3.4 to 13.5% RSD, except for one Compound (19.1% at low concern rations). The accuracy ranged from 96.9 to 115.4%. Matrix effects were compensated for by use of isotopically labelled internal standards. Most folates in rice extracts were stable when kept for 2 weeks at -20 and -80 degrees C, but degraded after three fireeze-thaw cycles or at 4 degrees C, even when stored protected from light. Our validated method offers a sensitive and versatile approach to determine the level of six different monoglutamate folates in a complex matrix as rice.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LC-MS/MS, Folates, Tri-enzyme treatment, Rice analysis
journal title
JOURNAL OF CHROMATOGRAPHY A
J. Chromatogr. A
volume
1215
issue
1-2
pages
125 - 132
publisher
Elsevier Science
place of publication
1000 AE AMSTERDAM, NETHERLANDS
Web of Science type
Article
Web of Science id
000261845500016
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.756 (2008)
JCR rank
6/70 (2008)
JCR quartile
1 (2008)
ISSN
0021-9673
DOI
10.1016/j.chroma.2008.11.004
language
English
UGent publication?
yes
classification
A1
id
513426
handle
http://hdl.handle.net/1854/LU-513426
date created
2009-03-03 12:09:33
date last changed
2009-03-04 15:35:41
@article{513426,
  abstract     = {An LC-MS/MS method has been developed and validated for the analysis of six monoglutamate folates in rice. Accurate determination of folates in rice seeds required the optimisation of an extraction procedure encompassing homogenisation, heat treatment, tri-enzyme treatment (alpha-amylase, protease and deconjugase), centrifugation and Ultrafiltration. The resulting extract was loaded onto a Polaris C 18-A column, followed by gradient elution and detection by tandem mass spectrometry. The validated method is sensitive, reproducible, accurate, and linear between 1 and 400 mu g/100 g rice (dry weight). The limit of detection and limit Of quantification in diluted rice matrix ranged between 0.2 and 1.2 mu g/100 g and between 0.6 and 4 mu g/100 g, respectively, depending on the Compound. Intra- and inter-day precision varied, respectively, from 1.0 to 9.1\% RSD, and from 3.4 to 13.5\% RSD, except for one Compound (19.1\% at low concern rations). The accuracy ranged from 96.9 to 115.4\%. Matrix effects were compensated for by use of isotopically labelled internal standards. Most folates in rice extracts were stable when kept for 2 weeks at -20 and -80 degrees C, but degraded after three fireeze-thaw cycles or at 4 degrees C, even when stored protected from light. Our validated method offers a sensitive and versatile approach to determine the level of six different monoglutamate folates in a complex matrix as rice.},
  author       = {De Brouwer, Veerle and Storojenko, Serguei and Van De Steene, Jet and Wille, Sarah and Van Der Straeten, Dominique and Lambert, Willy},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  keyword      = {LC-MS/MS,Folates,Tri-enzyme treatment,Rice analysis},
  language     = {eng},
  number       = {1-2},
  pages        = {125--132},
  publisher    = {Elsevier Science},
  title        = {Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.11.004},
  volume       = {1215},
  year         = {2008},
}

Chicago
De Brouwer, Veerle, Serguei Storojenko, Jet Van De Steene, Sarah Wille, Dominique Van Der Straeten, and Willy Lambert. 2008. “Optimisation and Validation of a Liquid Chromatography-tandem Mass Spectrometry Method for Folates in Rice.” Journal of Chromatography A 1215 (1-2): 125–132.
APA
De Brouwer, V., Storojenko, S., Van De Steene, J., Wille, S., Van Der Straeten, D., & Lambert, W. (2008). Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice. JOURNAL OF CHROMATOGRAPHY A, 1215(1-2), 125–132.
Vancouver
1.
De Brouwer V, Storojenko S, Van De Steene J, Wille S, Van Der Straeten D, Lambert W. Optimisation and validation of a liquid chromatography-tandem mass spectrometry method for folates in rice. JOURNAL OF CHROMATOGRAPHY A. 1000 AE AMSTERDAM, NETHERLANDS: Elsevier Science; 2008;1215(1-2):125–32.
MLA
De Brouwer, Veerle, Serguei Storojenko, Jet Van De Steene, et al. “Optimisation and Validation of a Liquid Chromatography-tandem Mass Spectrometry Method for Folates in Rice.” JOURNAL OF CHROMATOGRAPHY A 1215.1-2 (2008): 125–132. Print.