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Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells

Ryan Whitford UGent, Ana Fernandez Salina UGent, Ruth De Groodt UGent, Esther Ortega and Pierre Hilson UGent (2008) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 105(47). p.18625-18630
abstract
The Clavata3 (CLV3)/endosperm surrounding region (CLE) signaling peptides are encoded in large plant gene families. CLV3 and the other A-type CLE peptides promote cell differentiation in root and shoot apical meristems, whereas the B-type peptides (CLE41-CLE44) do not. Instead, CLE41 inhibits the differentiation of Zinnia elegans tracheary elements. To test whether CLE genes might code for antagonistic or synergistic functions, peptides from both types were combined through overexpression within or application onto Arabidopsis thaliana seedlings. The CLE41 peptide (CLE41p) promoted proliferation of vascular cells, although delaying differentiation into phloem and xylem cell lineages. Application of CLE41p or overexpression of CLE41 did not suppress the terminal differentiation of the root and shoot apices triggered by A-type CLE peptides. However, in combination, A-type peptides enhanced all of the phenotypes associated with CLE41 gain-of-function, leading to massive proliferation of vascular cells. This proliferation relied on auxin signaling because it was enhanced by exogenous application of a synthetic auxin, decreased by an auxin polar transport inhibitor, and abolished by a mutation in the Monopteros auxin response factor. These findings highlight that vascular patterning is a process controlled in time and space by different CLE peptides in conjunction with hormonal signaling.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
OF-FUNCTION PHENOTYPES, CLV3, CLAVATA3, cambium, hypocotyl, RAM, SAM, THALIANA, DIFFERENTIATION, FAMILY, ARABIDOPSIS ROOT-MERISTEM, GENE, EXPRESSION, TDIF, PROTEIN
journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Proc. Natl. Acad. Sci. USA
volume
105
issue
47
pages
18625 - 18630
Web of Science type
Article
Web of Science id
000261489300104
ISSN
0027-8424
DOI
10.1073/pnas.0809395105
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
506385
handle
http://hdl.handle.net/1854/LU-506385
date created
2009-02-27 13:54:50
date last changed
2013-10-11 15:05:40
@article{506385,
  abstract     = {The Clavata3 (CLV3)/endosperm surrounding region (CLE) signaling peptides are encoded in large plant gene families. CLV3 and the other A-type CLE peptides promote cell differentiation in root and shoot apical meristems, whereas the B-type peptides (CLE41-CLE44) do not. Instead, CLE41 inhibits the differentiation of Zinnia elegans tracheary elements. To test whether CLE genes might code for antagonistic or synergistic functions, peptides from both types were combined through overexpression within or application onto Arabidopsis thaliana seedlings. The CLE41 peptide (CLE41p) promoted proliferation of vascular cells, although delaying differentiation into phloem and xylem cell lineages. Application of CLE41p or overexpression of CLE41 did not suppress the terminal differentiation of the root and shoot apices triggered by A-type CLE peptides. However, in combination, A-type peptides enhanced all of the phenotypes associated with CLE41 gain-of-function, leading to massive proliferation of vascular cells. This proliferation relied on auxin signaling because it was enhanced by exogenous application of a synthetic auxin, decreased by an auxin polar transport inhibitor, and abolished by a mutation in the Monopteros auxin response factor. These findings highlight that vascular patterning is a process controlled in time and space by different CLE peptides in conjunction with hormonal signaling.},
  author       = {Whitford, Ryan and Fernandez Salina, Ana and De Groodt, Ruth and Ortega, Esther and Hilson, Pierre},
  issn         = {0027-8424},
  journal      = {PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA},
  keyword      = {OF-FUNCTION PHENOTYPES,CLV3,CLAVATA3,cambium,hypocotyl,RAM,SAM,THALIANA,DIFFERENTIATION,FAMILY,ARABIDOPSIS ROOT-MERISTEM,GENE,EXPRESSION,TDIF,PROTEIN},
  language     = {eng},
  number       = {47},
  pages        = {18625--18630},
  title        = {Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells},
  url          = {http://dx.doi.org/10.1073/pnas.0809395105},
  volume       = {105},
  year         = {2008},
}

Chicago
Whitford, Ryan, Ana Fernandez Salina, Ruth De Groodt, Esther Ortega, and Pierre Hilson. 2008. “Plant CLE Peptides from Two Distinct Functional Classes Synergistically Induce Division of Vascular Cells.” Proceedings of the National Academy of Sciences of the United States of America 105 (47): 18625–18630.
APA
Whitford, R., Fernandez Salina, A., De Groodt, R., Ortega, E., & Hilson, P. (2008). Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 105(47), 18625–18630.
Vancouver
1.
Whitford R, Fernandez Salina A, De Groodt R, Ortega E, Hilson P. Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2008;105(47):18625–30.
MLA
Whitford, Ryan, Ana Fernandez Salina, Ruth De Groodt, et al. “Plant CLE Peptides from Two Distinct Functional Classes Synergistically Induce Division of Vascular Cells.” PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 105.47 (2008): 18625–18630. Print.