FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast
- Author
- Scott Smukalla, Marina Caldara, Nathalie Pochet (UGent) , Anne Beauvais, Stephanie Guadagnini, Chen Yan, Marcelo D Vinces, An Jansen, Marie Christine Prevost, Jean-Paul Latgé, Gerald R Fink, Kevin R Foster and Kevin J Verstrepen
- Organization
- Abstract
- The budding yeast, Saccharomyces cerevisiae, has emerged as an archetype of eukaryotic cell biology. Here we show that S. cerevisiae is also a model for the evolution of cooperative behavior by revisiting flocculation, a self-adherence phenotype lacking in most laboratory strains. Expression of the gene FLO1 in the laboratory strain S288C restores flocculation, an altered physiological state, reminiscent of bacterial biofilms. Flocculation protects the FLO1 expressing cells from multiple stresses, including antimicrobials and ethanol. Furthermore, FLO1(+) cells avoid exploitation by nonexpressing flo1 cells by self/non-self recognition: FLO1(+) cells preferentially stick to one another, regardless of genetic relatedness across the rest of the genome. Flocculation, therefore, is driven by one of a few known "green beard genes,'' which direct cooperation toward other carriers of the same gene. Moreover, FLO1 is highly variable among strains both in expression and in sequence, suggesting that flocculation in S. cerevisiae is a dynamic, rapidly evolving social trait.
- Keywords
- CELL-SURFACE, EVOLUTION, SACCHAROMYCES-CEREVISIAE, ANTIFUNGAL AGENTS, RECOGNITION, EXPRESSION, RESISTANCE, CANDIDA, MICROORGANISMS, FLOCCULATION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-506227
- MLA
- Smukalla, Scott, et al. “FLO1 Is a Variable Green Beard Gene That Drives Biofilm-like Cooperation in Budding Yeast.” CELL, vol. 135, no. 4, 2008, pp. 726–37, doi:10.1016/j.cell.2008.09.037.
- APA
- Smukalla, S., Caldara, M., Pochet, N., Beauvais, A., Guadagnini, S., Yan, C., … Verstrepen, K. J. (2008). FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast. CELL, 135(4), 726–737. https://doi.org/10.1016/j.cell.2008.09.037
- Chicago author-date
- Smukalla, Scott, Marina Caldara, Nathalie Pochet, Anne Beauvais, Stephanie Guadagnini, Chen Yan, Marcelo D Vinces, et al. 2008. “FLO1 Is a Variable Green Beard Gene That Drives Biofilm-like Cooperation in Budding Yeast.” CELL 135 (4): 726–37. https://doi.org/10.1016/j.cell.2008.09.037.
- Chicago author-date (all authors)
- Smukalla, Scott, Marina Caldara, Nathalie Pochet, Anne Beauvais, Stephanie Guadagnini, Chen Yan, Marcelo D Vinces, An Jansen, Marie Christine Prevost, Jean-Paul Latgé, Gerald R Fink, Kevin R Foster, and Kevin J Verstrepen. 2008. “FLO1 Is a Variable Green Beard Gene That Drives Biofilm-like Cooperation in Budding Yeast.” CELL 135 (4): 726–737. doi:10.1016/j.cell.2008.09.037.
- Vancouver
- 1.Smukalla S, Caldara M, Pochet N, Beauvais A, Guadagnini S, Yan C, et al. FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast. CELL. 2008;135(4):726–37.
- IEEE
- [1]S. Smukalla et al., “FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast,” CELL, vol. 135, no. 4, pp. 726–737, 2008.
@article{506227, abstract = {{The budding yeast, Saccharomyces cerevisiae, has emerged as an archetype of eukaryotic cell biology. Here we show that S. cerevisiae is also a model for the evolution of cooperative behavior by revisiting flocculation, a self-adherence phenotype lacking in most laboratory strains. Expression of the gene FLO1 in the laboratory strain S288C restores flocculation, an altered physiological state, reminiscent of bacterial biofilms. Flocculation protects the FLO1 expressing cells from multiple stresses, including antimicrobials and ethanol. Furthermore, FLO1(+) cells avoid exploitation by nonexpressing flo1 cells by self/non-self recognition: FLO1(+) cells preferentially stick to one another, regardless of genetic relatedness across the rest of the genome. Flocculation, therefore, is driven by one of a few known "green beard genes,'' which direct cooperation toward other carriers of the same gene. Moreover, FLO1 is highly variable among strains both in expression and in sequence, suggesting that flocculation in S. cerevisiae is a dynamic, rapidly evolving social trait.}}, author = {{Smukalla, Scott and Caldara, Marina and Pochet, Nathalie and Beauvais, Anne and Guadagnini, Stephanie and Yan, Chen and Vinces, Marcelo D and Jansen, An and Prevost, Marie Christine and Latgé, Jean-Paul and Fink, Gerald R and Foster, Kevin R and Verstrepen, Kevin J}}, issn = {{0092-8674}}, journal = {{CELL}}, keywords = {{CELL-SURFACE,EVOLUTION,SACCHAROMYCES-CEREVISIAE,ANTIFUNGAL AGENTS,RECOGNITION,EXPRESSION,RESISTANCE,CANDIDA,MICROORGANISMS,FLOCCULATION}}, language = {{eng}}, number = {{4}}, pages = {{726--737}}, title = {{FLO1 is a variable green beard gene that drives biofilm-like cooperation in budding yeast}}, url = {{http://doi.org/10.1016/j.cell.2008.09.037}}, volume = {{135}}, year = {{2008}}, }
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