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Cellular and molecular requirements for polar PIN targeting and transcytosis in plants

Jürgen Kleine-Vehn UGent, Lukasz Langowski UGent, Justyna Wíśniewska, Pankaj Dhonukshe UGent, Philip Brewer and Jiri Friml UGent (2008) MOLECULAR PLANT. 1(6). p.1056-1066
abstract
The polar, sub-cellular localization of PIN auxin efflux carriers determines the direction of intercellular auxin flow, thus defining the spatial aspect of auxin signalling. Dynamic, transcytosis-like relocalizations of PIN proteins occur in response to external and internal signals, integrating these signals into changes in auxin distribution. Here, we examine the cellular and molecular mechanisms of polar PIN delivery and transcytosis. The mechanisms of the ARF-GEF-dependent polar targeting and transcytosis are well conserved and show little variations among diverse Arabidopsis ecotypes consistent with their fundamental importance in regulating plant development. At the cellular level, we refine previous findings on the role of the actin cytoskeleton in apical and basal PIN targeting, and identify a previously unknown role for microtubules, specifically in basal targeting. PIN protein delivery to different sides of the cell is mediated by ARF-dependent trafficking with a previously unknown complex level of distinct ARF-GEF vesicle trafficking regulators. Our data suggest that alternative recruitment of PIN proteins by these distinct pathways can account for cell type- and cargo-specific aspects of polar targeting, as well as for polarity changes in response to different signals. The resulting dynamic PIN positioning to different sides of cells defines a three-dimensional pattern of auxin fluxes within plant tissues.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
PATHWAY, PROTEIN, EFFLUX, LOCALIZATION, TRANSPORT, ARABIDOPSIS, polarity, protein targeting, GRAVITROPISM, CELLS, cytoskeleton, PERMEASE AUX1, DEPENDENT AUXIN GRADIENTS
journal title
MOLECULAR PLANT
Mol. Plant.
volume
1
issue
6
pages
1056 - 1066
Web of Science type
Article
Web of Science id
000262858000015
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
99.999 (2008)
JCR rank
1/276 (2008)
JCR quartile
4 (2008)
ISSN
1674-2052
DOI
10.1093/mp/ssn062
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
506009
handle
http://hdl.handle.net/1854/LU-506009
date created
2009-02-26 17:54:20
date last changed
2013-09-17 11:21:46
@article{506009,
  abstract     = {The polar, sub-cellular localization of PIN auxin efflux carriers determines the direction of intercellular auxin flow, thus defining the spatial aspect of auxin signalling. Dynamic, transcytosis-like relocalizations of PIN proteins occur in response to external and internal signals, integrating these signals into changes in auxin distribution. Here, we examine the cellular and molecular mechanisms of polar PIN delivery and transcytosis. The mechanisms of the ARF-GEF-dependent polar targeting and transcytosis are well conserved and show little variations among diverse Arabidopsis ecotypes consistent with their fundamental importance in regulating plant development. At the cellular level, we refine previous findings on the role of the actin cytoskeleton in apical and basal PIN targeting, and identify a previously unknown role for microtubules, specifically in basal targeting. PIN protein delivery to different sides of the cell is mediated by ARF-dependent trafficking with a previously unknown complex level of distinct ARF-GEF vesicle trafficking regulators. Our data suggest that alternative recruitment of PIN proteins by these distinct pathways can account for cell type- and cargo-specific aspects of polar targeting, as well as for polarity changes in response to different signals. The resulting dynamic PIN positioning to different sides of cells defines a three-dimensional pattern of auxin fluxes within plant tissues.},
  author       = {Kleine-Vehn, J{\"u}rgen and Langowski, Lukasz and W{\'i}\'{s}niewska, Justyna and Dhonukshe, Pankaj and Brewer, Philip and Friml, Jiri},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keyword      = {PATHWAY,PROTEIN,EFFLUX,LOCALIZATION,TRANSPORT,ARABIDOPSIS,polarity,protein targeting,GRAVITROPISM,CELLS,cytoskeleton,PERMEASE AUX1,DEPENDENT AUXIN GRADIENTS},
  language     = {eng},
  number       = {6},
  pages        = {1056--1066},
  title        = {Cellular and molecular requirements for polar PIN targeting and transcytosis in plants},
  url          = {http://dx.doi.org/10.1093/mp/ssn062},
  volume       = {1},
  year         = {2008},
}

Chicago
Kleine-Vehn, Jürgen, Lukasz Langowski, Justyna Wíśniewska, Pankaj Dhonukshe, Philip Brewer, and Jiri Friml. 2008. “Cellular and Molecular Requirements for Polar PIN Targeting and Transcytosis in Plants.” Molecular Plant 1 (6): 1056–1066.
APA
Kleine-Vehn, J., Langowski, L., Wíśniewska, J., Dhonukshe, P., Brewer, P., & Friml, J. (2008). Cellular and molecular requirements for polar PIN targeting and transcytosis in plants. MOLECULAR PLANT, 1(6), 1056–1066.
Vancouver
1.
Kleine-Vehn J, Langowski L, Wíśniewska J, Dhonukshe P, Brewer P, Friml J. Cellular and molecular requirements for polar PIN targeting and transcytosis in plants. MOLECULAR PLANT. 2008;1(6):1056–66.
MLA
Kleine-Vehn, Jürgen, Lukasz Langowski, Justyna Wíśniewska, et al. “Cellular and Molecular Requirements for Polar PIN Targeting and Transcytosis in Plants.” MOLECULAR PLANT 1.6 (2008): 1056–1066. Print.