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Development of a sensitive diagnostic multiplex platform based on digitally encoded microcarriers

Stefaan Derveaux UGent (2009)
abstract
In answer to the ever-increasing need in biomolecular research and clinical diagnostics to carry out many assays simultaneously in on tube, several microcarrier-based multiplex technologies (suspension arrays) have arisen in the past few years. Simultaneous detection of different target molecules that are present in one sample is possible by incubating the sample with a mixture of differently encoded microcarriers, each carrying another probe which can specifically interact with one of the targets. This means that each target will bind to a differently encoded microcarrier. When the targets are caught, several methods exist to label those 'positive' microcarriers. By means of this label, and by means of the code, it becomes possible to verify whether a target was caught at its surface, and which target was caught, respectively. Those multiplex measurements work quantitatively, because the more a target is present in the sample, the more targets will bind to their corresponding microcarrier. Five years ago, our research group proposed the use of spatial selective photobleaching, as an alternative method for the development of digitally encoded microcarriers, which were called 'memobeads'. It was suggested that this method could overcome the multiplexing limitations of existing technologies. The present study aimed to optimize the surface characteristics of t hose memobeads, and to verify whether they could then be applied to multiplex protein tests and nucleic acid tests. Furthermore, it was investigated in which way these memobead assays (and in general the assays performed with every kind of suspension arrays) could be improved to make them more efficient and sensitive.
Please use this url to cite or link to this publication:
author
promoter
UGent and UGent
organization
alternative title
Ontwikkeling van een gevoelig diagnostisch multiplex platform gebaseerd op digitaal gecodeerde micropartikels
year
type
dissertation (monograph)
subject
keyword
(bio)analytical assays, diagnostics, memobeads, digitally encoded microcarriers
pages
240 pages
place of publication
Gent
defense location
Het Pand, Onderbergen 1, 9000 Gent
defense date
2009-01-13 17:30
language
English
UGent publication?
yes
classification
D1
id
505472
handle
http://hdl.handle.net/1854/LU-505472
alternative location
http://lib.ugent.be/fulltxt/RUG01/001/325/188/RUG01-001325188_2010_0001_AC.pdf
date created
2009-02-25 13:03:04
date last changed
2009-02-26 08:32:38
@phdthesis{505472,
  abstract     = {In answer to the ever-increasing need in biomolecular research and clinical diagnostics to carry out many assays simultaneously in on tube, several microcarrier-based multiplex technologies (suspension arrays) have arisen in the past few years. Simultaneous detection of different target molecules that are present in one sample is possible by incubating the sample with a mixture of differently encoded microcarriers, each carrying another probe which can specifically interact with one of the targets. This means that each target will bind to a differently encoded microcarrier. When the targets are caught, several methods exist to label those 'positive' microcarriers. By means of this label, and by means of the code, it becomes possible to verify whether a target was caught at its surface, and which target was caught, respectively. Those multiplex measurements work quantitatively, because the more a target is present in the sample, the more targets will bind to their corresponding microcarrier. Five years ago, our research group proposed the use of spatial selective photobleaching, as an alternative method for the development of digitally encoded microcarriers, which were called 'memobeads'. It was suggested that this method could overcome the multiplexing limitations of existing technologies. The present study aimed to optimize the surface characteristics of t hose memobeads, and to verify whether they could then be applied to multiplex protein tests and nucleic acid tests. Furthermore, it was investigated in which way these memobead assays (and in general the assays performed with every kind of suspension arrays) could be improved to make them more efficient and sensitive.},
  author       = {Derveaux, Stefaan},
  keyword      = {(bio)analytical assays,diagnostics,memobeads,digitally encoded microcarriers},
  language     = {eng},
  pages        = {240},
  school       = {Ghent University},
  title        = {Development of a sensitive diagnostic multiplex platform based on digitally encoded microcarriers},
  url          = {http://lib.ugent.be/fulltxt/RUG01/001/325/188/RUG01-001325188\_2010\_0001\_AC.pdf},
  year         = {2009},
}

Chicago
Derveaux, Stefaan. 2009. “Development of a Sensitive Diagnostic Multiplex Platform Based on Digitally Encoded Microcarriers”. Gent.
APA
Derveaux, S. (2009). Development of a sensitive diagnostic multiplex platform based on digitally encoded microcarriers. Gent.
Vancouver
1.
Derveaux S. Development of a sensitive diagnostic multiplex platform based on digitally encoded microcarriers. [Gent]; 2009.
MLA
Derveaux, Stefaan. “Development of a Sensitive Diagnostic Multiplex Platform Based on Digitally Encoded Microcarriers.” 2009 : n. pag. Print.