Ghent University Academic Bibliography

Advanced

Kinetic modeling of a bi-enzymatic system for efficient conversion of lactose to lactobionic acid

Wouter Van Hecke UGent, Aditya Bhagwat UGent, Roland Ludwig, Jo Dewulf UGent, Dietmar Haltrich and Herman Van Langenhove UGent (2009) BIOTECHNOLOGY AND BIOENGINEERING. 102(5). p.1475-1482
abstract
A model has been developed to describe the interaction between two enzymes and an intermediary redox mediator. In this bi-enzymatic process, the enzyme cellobiose dehydrogenase oxidizes lactose at the C-1 position of the reducing sugar moiety to lactobionolactone, which spontaneously hydrolyzes to lactobionic acid. 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is used as electron acceptor and is continuously regenerated by laccase. Oxygen is the terminal electron acceptor and is fully reduced to water by laccase, a copper-containing oxidase. Oxygen is added to the system by means of bubble-free oxygenation. Using the model, the productivity of the process is investigated by simultaneous solution of the rate equations for varying enzyme quantities and redox mediator concentrations, solved with the aid of a numerical solution. The isocharts developed in this work provide an easy-to-use graphical tool to determine optimal process conditions. The model allows the optimization of the employed activities of the two enzymes and the redox mediator concentration for a given overall oxygen mass transfer coefficient by using the isocharts. Model predictions are well in agreement with the experimental data.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
carbohydrates, biotransformations, cellobiose dehydrogenase, kinetic modeling, laccase
journal title
BIOTECHNOLOGY AND BIOENGINEERING
Biotechnol. Bioeng.
volume
102
issue
5
pages
1475 - 1482
Web of Science type
Article
Web of Science id
000264126600021
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.377 (2009)
JCR rank
34/150 (2009)
JCR quartile
1 (2009)
ISSN
0006-3592
DOI
10.1002/bit.22165
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
505412
handle
http://hdl.handle.net/1854/LU-505412
date created
2009-02-25 10:47:37
date last changed
2011-10-17 10:32:30
@article{505412,
  abstract     = {A model has been developed to describe the interaction between two enzymes and an intermediary redox mediator. In this bi-enzymatic process, the enzyme cellobiose dehydrogenase oxidizes lactose at the C-1 position of the reducing sugar moiety to lactobionolactone, which spontaneously hydrolyzes to lactobionic acid. 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is used as electron acceptor and is continuously regenerated by laccase. Oxygen is the terminal electron acceptor and is fully reduced to water by laccase, a copper-containing oxidase. Oxygen is added to the system by means of bubble-free oxygenation. Using the model, the productivity of the process is investigated by simultaneous solution of the rate equations for varying enzyme quantities and redox mediator concentrations, solved with the aid of a numerical solution. The isocharts developed in this work provide an easy-to-use graphical tool to determine optimal process conditions. The model allows the optimization of the employed activities of the two enzymes and the redox mediator concentration for a given overall oxygen mass transfer coefficient by using the isocharts. Model predictions are well in agreement with the experimental data.},
  author       = {Van Hecke, Wouter and Bhagwat, Aditya and Ludwig, Roland and Dewulf, Jo and Haltrich, Dietmar and Van Langenhove, Herman},
  issn         = {0006-3592},
  journal      = {BIOTECHNOLOGY AND BIOENGINEERING},
  keyword      = {carbohydrates,biotransformations,cellobiose dehydrogenase,kinetic modeling,laccase},
  language     = {eng},
  number       = {5},
  pages        = {1475--1482},
  title        = {Kinetic modeling of a bi-enzymatic system for efficient conversion of lactose to lactobionic acid},
  url          = {http://dx.doi.org/10.1002/bit.22165},
  volume       = {102},
  year         = {2009},
}

Chicago
Van Hecke, Wouter, Aditya Bhagwat, Roland Ludwig, Jo Dewulf, Dietmar Haltrich, and Herman Van Langenhove. 2009. “Kinetic Modeling of a Bi-enzymatic System for Efficient Conversion of Lactose to Lactobionic Acid.” Biotechnology and Bioengineering 102 (5): 1475–1482.
APA
Van Hecke, W., Bhagwat, A., Ludwig, R., Dewulf, J., Haltrich, D., & Van Langenhove, H. (2009). Kinetic modeling of a bi-enzymatic system for efficient conversion of lactose to lactobionic acid. BIOTECHNOLOGY AND BIOENGINEERING, 102(5), 1475–1482.
Vancouver
1.
Van Hecke W, Bhagwat A, Ludwig R, Dewulf J, Haltrich D, Van Langenhove H. Kinetic modeling of a bi-enzymatic system for efficient conversion of lactose to lactobionic acid. BIOTECHNOLOGY AND BIOENGINEERING. 2009;102(5):1475–82.
MLA
Van Hecke, Wouter, Aditya Bhagwat, Roland Ludwig, et al. “Kinetic Modeling of a Bi-enzymatic System for Efficient Conversion of Lactose to Lactobionic Acid.” BIOTECHNOLOGY AND BIOENGINEERING 102.5 (2009): 1475–1482. Print.