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Bubble-free oxygenation of a bi-enzymatic system: effect on biocatalyst stability

Wouter Van Hecke UGent, Roland Ludwig, Jo Dewulf UGent, Markus Auly, Tom Messiaen, Dietmar Haltrich and Herman Van Langenhove UGent (2009) BIOTECHNOLOGY AND BIOENGINEERING. 102(1). p.122-131
abstract
The effect of bubble-free oxygenation on the stability of a bi-enzymatic system with redox mediator regeneration for the conversion of lactose to lactobionic acid was investigated in a miniaturized reactor with bubbleless oxygenation. Earlier investigations of this biocatalytic oxidation have shown that the dispersive addition of oxygen can cause significant enzyme inactivation. In the process studied, the enzyme cellobiose dehydrogenase (CDH) oxidizes lactose at the C-1 position of the reducing sugar moiety to lactobionolactone, which spontaneously hydrolyzes to lactobionic acid. 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt was used as electron acceptor for CDH and was continuously regenerated (reoxidized) by laccase, a blue multi-copper oxidase. Oxygen served as the terminal electron acceptor of the reaction and was fully reduced to water by laccase. The overall mass transfer coefficient of the miniaturized reactor was determined at 30 and 45°C; conversions were conducted both in the reaction-limited and diffusion-limited regime to study catalyst inactivation. The bubbleless oxygenation was successful in avoiding gas/liquid interface inactivation. It was also shown that the oxidized redox mediator plays a key role in the inactivation mechanism of the biocatalysts unobserved during previous studies.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
laccase-mediator system, cellobiose dehydrogenase, bubble-free oxygenation, composite membrane, lactobionic acid
journal title
BIOTECHNOLOGY AND BIOENGINEERING
Biotechnol. Bioeng.
volume
102
issue
1
pages
122 - 131
publisher
Wiley Interscience
Web of Science type
Article
Web of Science id
000261826000015
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.377 (2009)
JCR rank
34/150 (2009)
JCR quartile
1 (2009)
ISSN
0006-3592
DOI
10.1002/bit.22042
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
504466
handle
http://hdl.handle.net/1854/LU-504466
alternative location
http://www3.interscience.wiley.com/journal/120835218/abstract
date created
2009-02-21 11:51:04
date last changed
2009-06-18 19:47:16
@article{504466,
  abstract     = {The effect of bubble-free oxygenation on the stability of a bi-enzymatic system with redox mediator regeneration for the conversion of lactose to lactobionic acid was investigated in a miniaturized reactor with bubbleless oxygenation. Earlier investigations of this biocatalytic oxidation have shown that the dispersive addition of oxygen can cause significant enzyme inactivation. In the process studied, the enzyme cellobiose dehydrogenase (CDH) oxidizes lactose at the C-1 position of the reducing sugar moiety to lactobionolactone, which spontaneously hydrolyzes to lactobionic acid. 2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt was used as electron acceptor for CDH and was continuously regenerated (reoxidized) by laccase, a blue multi-copper oxidase. Oxygen served as the terminal electron acceptor of the reaction and was fully reduced to water by laccase. The overall mass transfer coefficient of the miniaturized reactor was determined at 30 and 45{\textdegree}C; conversions were conducted both in the reaction-limited and diffusion-limited regime to study catalyst inactivation. The bubbleless oxygenation was successful in avoiding gas/liquid interface inactivation. It was also shown that the oxidized redox mediator plays a key role in the inactivation mechanism of the biocatalysts unobserved during previous studies.},
  author       = {Van Hecke, Wouter and Ludwig, Roland and Dewulf, Jo and Auly, Markus and Messiaen, Tom and Haltrich, Dietmar and Van Langenhove, Herman},
  issn         = {0006-3592},
  journal      = {BIOTECHNOLOGY AND BIOENGINEERING},
  keyword      = {laccase-mediator system,cellobiose dehydrogenase,bubble-free oxygenation,composite membrane,lactobionic acid},
  language     = {eng},
  number       = {1},
  pages        = {122--131},
  publisher    = {Wiley Interscience},
  title        = {Bubble-free oxygenation of a bi-enzymatic system: effect on biocatalyst stability},
  url          = {http://dx.doi.org/10.1002/bit.22042},
  volume       = {102},
  year         = {2009},
}

Chicago
Van Hecke, Wouter, Roland Ludwig, Jo Dewulf, Markus Auly, Tom Messiaen, Dietmar Haltrich, and Herman Van Langenhove. 2009. “Bubble-free Oxygenation of a Bi-enzymatic System: Effect on Biocatalyst Stability.” Biotechnology and Bioengineering 102 (1): 122–131.
APA
Van Hecke, W., Ludwig, R., Dewulf, J., Auly, M., Messiaen, T., Haltrich, D., & Van Langenhove, H. (2009). Bubble-free oxygenation of a bi-enzymatic system: effect on biocatalyst stability. BIOTECHNOLOGY AND BIOENGINEERING, 102(1), 122–131.
Vancouver
1.
Van Hecke W, Ludwig R, Dewulf J, Auly M, Messiaen T, Haltrich D, et al. Bubble-free oxygenation of a bi-enzymatic system: effect on biocatalyst stability. BIOTECHNOLOGY AND BIOENGINEERING. Wiley Interscience; 2009;102(1):122–31.
MLA
Van Hecke, Wouter, Roland Ludwig, Jo Dewulf, et al. “Bubble-free Oxygenation of a Bi-enzymatic System: Effect on Biocatalyst Stability.” BIOTECHNOLOGY AND BIOENGINEERING 102.1 (2009): 122–131. Print.