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Gene discovery: Polymorphism association with obsessive-compulsive disorder and proteome analysis of Artemisia annua.

(2007)
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Abstract
PART I: Polymorphism association with Obsessive-Compulsive Disorder In light of the putative role of the serotonergic, dopaminergic and possibly (nor)adrenergic system in OCD, following polymorphisms were analysed in a sample of >100 OCD patients and a control sample of >100 ethnically matched Caucasian subjects by means of a case-control study: Taq IA polymorphism in the non-coding region flanking the 3’ end of the dopamine D2 receptor (DRD2) gene; Catechol-O-Methyl Transferase (COMT) NlaIII high/low activity polymorphism; 5-HT2A 1438 A/G polymorphism; 5-HT1Dβ G861C polymorphism and the 5-HTTLPR 44-bp deletioninsertion. There was a higher frequency of the DRD2 A2 allele (p = 0.020) and a higher frequency of the low-activity COMT allele (p = 0.035) in male OCD patients compared to male controls. In addition, we observed an association of the DRD2 A2A2 genotype in patients with an early onset of OCD (p= 0.033). We studied whether the serotonin polymorphisms affect the efficacy of venlafaxine and paroxetine treatment in OCD. The response in paroxetine treated patients is associated with the GG genotype of the 5-HT2A polymorphism (χ2 = 8.66, df=2, p=0.013). In venlafaxine treated patients, response is associated with the SL genotype of the 5-HTTLPR polymorphism (χ2 = 9.71, df=2, p=0.008). PART II: proteomics on Artemisia annua Three strategies were followed to discover genes of the plant Artemisia annua L. that are involved in the production of the antimalarial artemisinin: a proteome analysis, a quantitative cDNA amplified fragment length polymorphism analysis (cDNA AFLP) and the construction of full length Expressed Sequence Tag (EST) cDNA libraries. We identified proteins that were differentially expressed between trichomes and whole leaf tissue. Proteins that are upregulated in the trichomes might be involved in artemisinin production. To narrow down the results to the most valid gene candidates, the proteome data was compared with a cDNA AFLP analysis that investigated samples of A. annua leafs, taken at different time points during a 72h time period after exposure to jasmonic acid. We were able to compile a list of EST candidates, which could be useful for further investigation.

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Citation

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Chicago
Van Nieuwerburgh, Filip. 2007. “Gene Discovery: Polymorphism Association with Obsessive-compulsive Disorder and Proteome Analysis of Artemisia Annua.”
APA
Van Nieuwerburgh, F. (2007). Gene discovery: Polymorphism association with obsessive-compulsive disorder and proteome analysis of Artemisia annua.
Vancouver
1.
Van Nieuwerburgh F. Gene discovery: Polymorphism association with obsessive-compulsive disorder and proteome analysis of Artemisia annua. 2007.
MLA
Van Nieuwerburgh, Filip. “Gene Discovery: Polymorphism Association with Obsessive-compulsive Disorder and Proteome Analysis of Artemisia Annua.” 2007 : n. pag. Print.
@phdthesis{470691,
  abstract     = {PART I: Polymorphism association with Obsessive-Compulsive Disorder In light of the putative role of the serotonergic, dopaminergic and possibly (nor)adrenergic system in OCD, following polymorphisms were analysed in a sample of {\textrangle}100 OCD patients and a control sample of {\textrangle}100 ethnically matched Caucasian subjects by means of a case-control study: Taq IA polymorphism in the non-coding region flanking the 3{\textquoteright} end of the dopamine D2 receptor (DRD2) gene; Catechol-O-Methyl Transferase (COMT) NlaIII high/low activity polymorphism; 5-HT2A 1438 A/G polymorphism; 5-HT1D\ensuremath{\beta} G861C polymorphism and the 5-HTTLPR 44-bp deletion\unmatched{f02f}insertion. There was a higher frequency of the DRD2 A2 allele (p = 0.020) and a higher frequency of the low-activity COMT allele (p = 0.035) in male OCD patients compared to male controls. In addition, we observed an association of the DRD2 A2A2 genotype in patients with an early onset of OCD (p= 0.033). We studied whether the serotonin polymorphisms affect the efficacy of venlafaxine and paroxetine treatment in OCD. The response in paroxetine treated patients is associated with the GG genotype of the 5-HT2A polymorphism (\ensuremath{\chi}2 = 8.66, df=2, p=0.013). In venlafaxine treated patients, response is associated with the SL genotype of the 5-HTTLPR polymorphism (\ensuremath{\chi}2 = 9.71, df=2, p=0.008). PART II: proteomics on Artemisia annua Three strategies were followed to discover genes of the plant Artemisia annua L. that are involved in the production of the antimalarial artemisinin: a proteome analysis, a quantitative cDNA amplified fragment length polymorphism analysis (cDNA AFLP) and the construction of full length Expressed Sequence Tag (EST) cDNA libraries. We identified proteins that were differentially expressed between trichomes and whole leaf tissue. Proteins that are upregulated in the trichomes might be involved in artemisinin production. To narrow down the results to the most valid gene candidates, the proteome data was compared with a cDNA AFLP analysis that investigated samples of A. annua leafs, taken at different time points during a 72h time period after exposure to jasmonic acid. We were able to compile a list of EST candidates, which could be useful for further investigation.},
  author       = {Van Nieuwerburgh, Filip},
  school       = {Ghent University},
  title        = {Gene discovery: Polymorphism association with obsessive-compulsive disorder and proteome analysis of Artemisia annua.},
  url          = {http://dx.doi.org/1854/7056},
  year         = {2007},
}

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