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Optimisation of Raman spectroscopy for the analysis of Basidiomycota: spores, latex and mycelium

Kris De Gussem (2007)
abstract
For several decades, Raman spectroscopy has been used in different research fields. As a result of progression in the instrumentation during the last years, Raman spectroscopy became available for the analysis of complex biomaterials. In this work, the opportunities of the technique for the analysis of Basidiomycota are explored. The chemical composition of spores of Lactarius species is studied. These spores are mostly comprised of lipids and saccharides: they contain large amounts of triolein and smaller amounts of amylopectin (amyloid ornamention) and chitin. Furthermore, the Raman spectra are compared with those of spores of the genera Collybia, Laccaria, Mycena and Russula. By using a chemometric identification protocol (interference elimination, autoscaling, PCA and LDA) the genus was correctly assigned to a spore print in 90% of the cases, whereas the species could not be deduced from the Raman spectra. It is shown that Raman spectroscopy is a good additional method for the analysis of Lactarius latex. 2D correlation analysis of the latex spectra gives an overview of the major compositional changes and may discriminate between the different reaction steps. The reaction sequence of Lactarius lacunarum latex is studied. Different types of reaction sequences are observed in different species, which might be of taxonomic importance. The last section of this work discusses an explorative study of mycelium cultures of common wood rots. The Raman spectra are discussed and compared with the Raman spectra of infected and unifected Fagus and Pinus wood. In this way, it was shown that hyphae of a wood rot fungus can be detected in infected wood, although the spectral differences in cellulose and lignin bands can be much larger relative to the bands of the fungus. White rots can be detected through the presence of several Raman bands (e.g. at 916 and between 1000 and 1008 cm-1), whereas carotenoid vibration bands between 1510 and 1540 cm-1 are typical for brown rots.
Please use this url to cite or link to this publication:
author
promoter
UGent
organization
alternative title
Optimalisatie van Ramanspectroscopie voor de analyse van Basidiomycota : sporen, latex en mycelium
year
type
dissertation (monograph)
subject
pages
180 pages
publisher
Ghent University. Faculty of Sciences
place of publication
Ghent, Belgium
language
English
UGent publication?
yes
classification
D1
copyright statement
I have retained and own the full copyright for this publication
id
469452
handle
http://hdl.handle.net/1854/LU-469452
alternative location
http://lib.ugent.be/fulltxt/RUG01/001/221/111/RUG01-001221111_2010_0001_AC.pdf
date created
2008-02-07 09:06:15
date last changed
2009-09-16 14:55:48
@phdthesis{469452,
  abstract     = {For several decades, Raman spectroscopy has been used in different research fields. As a result of progression in the instrumentation during the last years, Raman spectroscopy became available for the analysis of complex biomaterials. In this work, the opportunities of the technique for the analysis of Basidiomycota are explored. The chemical composition of spores of Lactarius species is studied. These spores are mostly comprised of lipids and saccharides: they contain large amounts of triolein and smaller amounts of amylopectin (amyloid ornamention) and chitin. Furthermore, the Raman spectra are compared with those of spores of the genera Collybia, Laccaria, Mycena and Russula. By using a chemometric identification protocol (interference elimination, autoscaling, PCA and LDA) the genus was correctly assigned to a spore print in 90\% of the cases, whereas the species could not be deduced from the Raman spectra. It is shown that Raman spectroscopy is a good additional method for the analysis of Lactarius latex. 2D correlation analysis of the latex spectra gives an overview of the major compositional changes and may discriminate between the different reaction steps. The reaction sequence of Lactarius lacunarum latex is studied. Different types of reaction sequences are observed in different species, which might be of taxonomic importance. The last section of this work discusses an explorative study of mycelium cultures of common wood rots. The Raman spectra are discussed and compared with the Raman spectra of infected and unifected Fagus and Pinus wood. In this way, it was shown that hyphae of a wood rot fungus can be detected in infected wood, although the spectral differences in cellulose and lignin bands can be much larger relative to the bands of the fungus. White rots can be detected through the presence of several Raman bands (e.g. at 916 and between 1000 and 1008 cm-1), whereas carotenoid vibration bands between 1510 and 1540 cm-1 are typical for brown rots.},
  author       = {De Gussem, Kris},
  language     = {eng},
  pages        = {180},
  publisher    = {Ghent University. Faculty of Sciences},
  school       = {Ghent University},
  title        = {Optimisation of Raman spectroscopy for the analysis of Basidiomycota: spores, latex and mycelium},
  url          = {http://lib.ugent.be/fulltxt/RUG01/001/221/111/RUG01-001221111\_2010\_0001\_AC.pdf},
  year         = {2007},
}

Chicago
De Gussem, Kris. 2007. “Optimisation of Raman Spectroscopy for the Analysis of Basidiomycota: Spores, Latex and Mycelium”. Ghent, Belgium: Ghent University. Faculty of Sciences.
APA
De Gussem, K. (2007). Optimisation of Raman spectroscopy for the analysis of Basidiomycota: spores, latex and mycelium. Ghent University. Faculty of Sciences, Ghent, Belgium.
Vancouver
1.
De Gussem K. Optimisation of Raman spectroscopy for the analysis of Basidiomycota: spores, latex and mycelium. [Ghent, Belgium]: Ghent University. Faculty of Sciences; 2007.
MLA
De Gussem, Kris. “Optimisation of Raman Spectroscopy for the Analysis of Basidiomycota: Spores, Latex and Mycelium.” 2007 : n. pag. Print.