Ghent University Academic Bibliography

Advanced

Can ultrasound solve the transport barrier of the neural retina?

Liesbeth Peeters, Ine Lentacker UGent, Roosmarijn Vandenbroucke UGent, Bart Lucas UGent, Jo Demeester UGent, Niek Sanders UGent and Stefaan De Smedt UGent (2008) PHARMACEUTICAL RESEARCH. 25(11). p.2657-2665
abstract
Purpose. Intravitreal injection of nonviral gene complexes may be promising in the treatment of retinal diseases. This study investigates the permeation of lipoplexes and polystyrene nanospheres through the neural retina and their uptake by the retinal pigment epithelium (RPE) either with or without ultrasound application. Materials and Methods. Anterior parts and vitreous of bovine eyes were removed. The neural retina was left intact or peeled away from the RPE. (Non)pegylated lipoplexes and pegylated nanospheres were applied. After 2 h incubation, the RPE cells were detached and analyzed for particle uptake by flow cytometry and confocal microscopy. Results. The neural retina is a significant transport barrier for pegylated nanospheres and (non)pegylated lipoplexes. Applying ultrasound improved the permeation of the nanoparticles up to 130 nm. Conclusions. Delivery of liposomal DNA complexes to the RPE cells is strongly limited by the neural retina. Ultrasound energy may be a useful tool to improve the neural retina permeability, given the nucleic acid carriers are small enough. Our results underline the importance to design and develop very small carriers for the delivery of nucleic acids to the neural retina and the RPE after intravitreal injection.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
non viral gene therapy, lipoplexes, ocular drug delivery, pegylation, ultrasound, MEDIATED GENE-TRANSFER, RECOMBINANT ADENOASSOCIATED VIRUS, DRUG-DELIVERY, IN-VIVO, TRANSGENE EXPRESSION, LOADED MICROBUBBLES, CHILDHOOD BLINDNESS, CYSTIC-FIBROSIS, VIRAL VECTORS, CANINE MODEL
journal title
PHARMACEUTICAL RESEARCH
Pharm. Res.
volume
25
issue
11
pages
2657 - 2665
Web of Science type
Article
Web of Science id
000259742300020
JCR category
CHEMISTRY, MULTIDISCIPLINARY
JCR impact factor
4.024 (2008)
JCR rank
20/125 (2008)
JCR quartile
1 (2008)
ISSN
0724-8741
DOI
10.1007/s11095-008-9684-2
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
446709
handle
http://hdl.handle.net/1854/LU-446709
date created
2009-01-15 13:34:00
date last changed
2012-10-10 10:08:32
@article{446709,
  abstract     = {Purpose. Intravitreal injection of nonviral gene complexes may be promising in the treatment of retinal diseases. This study investigates the permeation of lipoplexes and polystyrene nanospheres through the neural retina and their uptake by the retinal pigment epithelium (RPE) either with or without ultrasound application. 
Materials and Methods. Anterior parts and vitreous of bovine eyes were removed. The neural retina was left intact or peeled away from the RPE. (Non)pegylated lipoplexes and pegylated nanospheres were applied. After 2 h incubation, the RPE cells were detached and analyzed for particle uptake by flow cytometry and confocal microscopy. 
Results. The neural retina is a significant transport barrier for pegylated nanospheres and (non)pegylated lipoplexes. Applying ultrasound improved the permeation of the nanoparticles up to 130 nm. 
Conclusions. Delivery of liposomal DNA complexes to the RPE cells is strongly limited by the neural retina. Ultrasound energy may be a useful tool to improve the neural retina permeability, given the nucleic acid carriers are small enough. Our results underline the importance to design and develop very small carriers for the delivery of nucleic acids to the neural retina and the RPE after intravitreal injection.},
  author       = {Peeters, Liesbeth and Lentacker, Ine and Vandenbroucke, Roosmarijn and Lucas, Bart and Demeester, Jo and Sanders, Niek and De Smedt, Stefaan},
  issn         = {0724-8741},
  journal      = {PHARMACEUTICAL RESEARCH},
  keyword      = {non viral gene therapy,lipoplexes,ocular drug delivery,pegylation,ultrasound,MEDIATED GENE-TRANSFER,RECOMBINANT ADENOASSOCIATED VIRUS,DRUG-DELIVERY,IN-VIVO,TRANSGENE EXPRESSION,LOADED MICROBUBBLES,CHILDHOOD BLINDNESS,CYSTIC-FIBROSIS,VIRAL VECTORS,CANINE MODEL},
  language     = {eng},
  number       = {11},
  pages        = {2657--2665},
  title        = {Can ultrasound solve the transport barrier of the neural retina?},
  url          = {http://dx.doi.org/10.1007/s11095-008-9684-2},
  volume       = {25},
  year         = {2008},
}

Chicago
Peeters, Liesbeth, Ine Lentacker, Roosmarijn Vandenbroucke, Bart Lucas, Jo Demeester, Niek Sanders, and Stefaan De Smedt. 2008. “Can Ultrasound Solve the Transport Barrier of the Neural Retina?” Pharmaceutical Research 25 (11): 2657–2665.
APA
Peeters, Liesbeth, Lentacker, I., Vandenbroucke, R., Lucas, B., Demeester, J., Sanders, N., & De Smedt, S. (2008). Can ultrasound solve the transport barrier of the neural retina? PHARMACEUTICAL RESEARCH, 25(11), 2657–2665.
Vancouver
1.
Peeters L, Lentacker I, Vandenbroucke R, Lucas B, Demeester J, Sanders N, et al. Can ultrasound solve the transport barrier of the neural retina? PHARMACEUTICAL RESEARCH. 2008;25(11):2657–65.
MLA
Peeters, Liesbeth, Ine Lentacker, Roosmarijn Vandenbroucke, et al. “Can Ultrasound Solve the Transport Barrier of the Neural Retina?” PHARMACEUTICAL RESEARCH 25.11 (2008): 2657–2665. Print.