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Implementation of gas chromatography combined with simultaneously selected ion monitoring and full scan mass spectrometry in doping analysis

Wim Van Thuyne (UGent) , R VAN EENOO and Frans Delbeke (UGent)
(2008) JOURNAL OF CHROMATOGRAPHY A. 1210(2). p.193-202
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Abstract
A comprehensive screening method for the detection of prohibited substances in doping control is described and validated. This method is capable of detecting over 150 components mentioned on the list of the World Anti-Doping Agency including anabolic androgenic steroids, stimulants and all narcotic agents that are currently analysed using different analytical methods. The analytes are extracted from urine by a combined extraction procedure using freshly distilled diethyl ether and tert-butyl methyl ether as extraction solvents at pH 9.5 and 14 respectively. Prior to GC-MS analysis the residues are combined and derivatised using a mixture of N-methyl-N-trimethylsilyltrifluoroacetamide, NH4I and ethanethiol. The mass spectrometer is simultaneously operated in the full scan mode (mass range varies along with GC-oven temperature program) and in the selected ion monitoring mode. The obtained limits of detection are in compliance with the requirements set by the World Anti-Doping Agency. Besides narcotics, stimulants and anabolic androgenic agents, this method is also capable of detecting several agents with anti-estrogenic activity and some beta-agonists. This comprehensive screening method reduces the amount of urine needed and increases the sample throughput without a loss in sensitivity and selectivity.

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Chicago
Van Thuyne, Wim, R VAN EENOO, and Frans Delbeke. 2008. “Implementation of Gas Chromatography Combined with Simultaneously Selected Ion Monitoring and Full Scan Mass Spectrometry in Doping Analysis.” Journal of Chromatography A 1210 (2): 193–202.
APA
Van Thuyne, Wim, VAN EENOO, R., & Delbeke, F. (2008). Implementation of gas chromatography combined with simultaneously selected ion monitoring and full scan mass spectrometry in doping analysis. JOURNAL OF CHROMATOGRAPHY A, 1210(2), 193–202.
Vancouver
1.
Van Thuyne W, VAN EENOO R, Delbeke F. Implementation of gas chromatography combined with simultaneously selected ion monitoring and full scan mass spectrometry in doping analysis. JOURNAL OF CHROMATOGRAPHY A. 2008;1210(2):193–202.
MLA
Van Thuyne, Wim, R VAN EENOO, and Frans Delbeke. “Implementation of Gas Chromatography Combined with Simultaneously Selected Ion Monitoring and Full Scan Mass Spectrometry in Doping Analysis.” JOURNAL OF CHROMATOGRAPHY A 1210.2 (2008): 193–202. Print.
@article{446268,
  abstract     = {A comprehensive screening method for the detection of prohibited substances in doping control is described and validated. This method is capable of detecting over 150 components mentioned on the list of the World Anti-Doping Agency including anabolic androgenic steroids, stimulants and all narcotic agents that are currently analysed using different analytical methods. The analytes are extracted from urine by a combined extraction procedure using freshly distilled diethyl ether and tert-butyl methyl ether as extraction solvents at pH 9.5 and 14 respectively. Prior to GC-MS analysis the residues are combined and derivatised using a mixture of N-methyl-N-trimethylsilyltrifluoroacetamide, NH4I and ethanethiol. The mass spectrometer is simultaneously operated in the full scan mode (mass range varies along with GC-oven temperature program) and in the selected ion monitoring mode. The obtained limits of detection are in compliance with the requirements set by the World Anti-Doping Agency. Besides narcotics, stimulants and anabolic androgenic agents, this method is also capable of detecting several agents with anti-estrogenic activity and some beta-agonists. This comprehensive screening method reduces the amount of urine needed and increases the sample throughput without a loss in sensitivity and selectivity.},
  author       = {Van Thuyne, Wim and VAN EENOO, R and Delbeke, Frans},
  issn         = {0021-9673},
  journal      = {JOURNAL OF CHROMATOGRAPHY A},
  language     = {eng},
  number       = {2},
  pages        = {193--202},
  title        = {Implementation of gas chromatography combined with simultaneously selected ion monitoring and full scan mass spectrometry in doping analysis},
  url          = {http://dx.doi.org/10.1016/j.chroma.2008.09.049},
  volume       = {1210},
  year         = {2008},
}

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