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The DOF transcription factor OBP1 is involved in cell cycle regulation in Arabidopsis thaliana

Aleksandra Skirycz UGent, Amandine Radziejwoski UGent, Wolfgang Busch, Matthew A Hannah, Joanna Czeszejko, Mirosław Kwaśniewski, Maria-Inès Zanor, Jan U Lohmann, Lieven De Veylder UGent, Isabell Witt, et al. (2008) PLANT JOURNAL. 56(5). p.779-792
abstract
In contrast to animal growth, plant growth is largely post-embryonic. Therefore plants have developed new mechanisms to precisely regulate cell proliferation by means of internal and external stimuli whilst the general core cell cycle machinery is conserved between eukaryotes. In this work we demonstrate a role for the Arabidopsis thaliana DNA-binding-with-one-finger (DOF) transcription factor OBP1 in the control of cell division upon developmental signalling. Inducible overexpression of OBP1 resulted in a significant overrepresentation of cell cycle genes among the upregulated transcripts. Direct targets of OBP1, as verified by chromatin immunoprecipitation, include at least the core cell cycle gene CYCD3;3 and the replication-specific transcription factor gene AtDOF2;3. Consistent with our molecular data, short-term activation of OBP1 in cell cultures affected cell cycle re-entry, shortening the duration of the G(1) phase and the overall length of the cell cycle, whilst constitutive overexpression of OBP1 in plants influenced cell size and cell number, leading to a dwarfish phenotype. Expression during embryogenesis, germination and lateral root initiation suggests an important role for OBP1 in cell cycle re-entry, operating as a transcriptional regulator of key cell cycle genes. Our findings provide significant input into our understanding of how cell cycle activity is incorporated into plant growth and development.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
DOF transcription factor, cell cycle, Arabidopsis, OBP1, D-type cyclin, GENOME-WIDE ANALYSIS, RETINOBLASTOMA-RELATED PROTEIN, DEPENDENT KINASE COMPLEX, GENE-EXPRESSION, DOMAIN PROTEINS, SUSPENSION-CULTURES, SALICYLIC-ACID, TOBACCO, GROWTH, DIVISION
journal title
PLANT JOURNAL
Plant J.
volume
56
issue
5
pages
779 - 792
Web of Science type
Article
Web of Science id
000261275400008
JCR category
PLANT SCIENCES
JCR impact factor
6.493 (2008)
JCR rank
10/155 (2008)
JCR quartile
1 (2008)
ISSN
0960-7412
DOI
10.1111/j.1365-313X.2008.03641.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
445513
handle
http://hdl.handle.net/1854/LU-445513
date created
2008-12-24 02:25:00
date last changed
2016-12-19 15:40:41
@article{445513,
  abstract     = {In contrast to animal growth, plant growth is largely post-embryonic. Therefore plants have developed new mechanisms to precisely regulate cell proliferation by means of internal and external stimuli whilst the general core cell cycle machinery is conserved between eukaryotes. In this work we demonstrate a role for the Arabidopsis thaliana DNA-binding-with-one-finger (DOF) transcription factor OBP1 in the control of cell division upon developmental signalling. Inducible overexpression of OBP1 resulted in a significant overrepresentation of cell cycle genes among the upregulated transcripts. Direct targets of OBP1, as verified by chromatin immunoprecipitation, include at least the core cell cycle gene CYCD3;3 and the replication-specific transcription factor gene AtDOF2;3. Consistent with our molecular data, short-term activation of OBP1 in cell cultures affected cell cycle re-entry, shortening the duration of the G(1) phase and the overall length of the cell cycle, whilst constitutive overexpression of OBP1 in plants influenced cell size and cell number, leading to a dwarfish phenotype. Expression during embryogenesis, germination and lateral root initiation suggests an important role for OBP1 in cell cycle re-entry, operating as a transcriptional regulator of key cell cycle genes. Our findings provide significant input into our understanding of how cell cycle activity is incorporated into plant growth and development.},
  author       = {Skirycz, Aleksandra and Radziejwoski, Amandine and Busch, Wolfgang and Hannah, Matthew A and Czeszejko, Joanna and Kwa\'{s}niewski, Miros\unmatched{0142}aw and Zanor, Maria-In{\`e}s and Lohmann, Jan U and De Veylder, Lieven and Witt, Isabell and Mueller-Roeber, Bernd},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  keyword      = {DOF transcription factor,cell cycle,Arabidopsis,OBP1,D-type cyclin,GENOME-WIDE ANALYSIS,RETINOBLASTOMA-RELATED PROTEIN,DEPENDENT KINASE COMPLEX,GENE-EXPRESSION,DOMAIN PROTEINS,SUSPENSION-CULTURES,SALICYLIC-ACID,TOBACCO,GROWTH,DIVISION},
  language     = {eng},
  number       = {5},
  pages        = {779--792},
  title        = {The DOF transcription factor OBP1 is involved in cell cycle regulation in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.1111/j.1365-313X.2008.03641.x},
  volume       = {56},
  year         = {2008},
}

Chicago
Skirycz, Aleksandra, Amandine Radziejwoski, Wolfgang Busch, Matthew A Hannah, Joanna Czeszejko, Mirosław Kwaśniewski, Maria-Inès Zanor, et al. 2008. “The DOF Transcription Factor OBP1 Is Involved in Cell Cycle Regulation in Arabidopsis Thaliana.” Plant Journal 56 (5): 779–792.
APA
Skirycz, A., Radziejwoski, A., Busch, W., Hannah, M. A., Czeszejko, J., Kwaśniewski, M., Zanor, M.-I., et al. (2008). The DOF transcription factor OBP1 is involved in cell cycle regulation in Arabidopsis thaliana. PLANT JOURNAL, 56(5), 779–792.
Vancouver
1.
Skirycz A, Radziejwoski A, Busch W, Hannah MA, Czeszejko J, Kwaśniewski M, et al. The DOF transcription factor OBP1 is involved in cell cycle regulation in Arabidopsis thaliana. PLANT JOURNAL. 2008;56(5):779–92.
MLA
Skirycz, Aleksandra, Amandine Radziejwoski, Wolfgang Busch, et al. “The DOF Transcription Factor OBP1 Is Involved in Cell Cycle Regulation in Arabidopsis Thaliana.” PLANT JOURNAL 56.5 (2008): 779–792. Print.