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ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

(2008) EMBO JOURNAL. 27(20). p.2746-2756
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Abstract
In multicellular organisms, organogenesis requires a tight control of the balance between cell division and cell differentiation. Distinct signalling pathways that connect both cellular processes with developmental cues might have evolved to suit different developmental plans. Here, we identified and characterized a novel protein that interacts with pre-replication complex (pre-RC) subunits, designated Armadillo BTB Arabidopsis protein 1 (ABAP1). Overexpression of ABAP1 in plants limited mitotic DNA replication and decreased cell proliferation in leaves, whereas ABAP1 downregulation increased cell division rates. Activity of ABAP1 in transcription was supported by its association with the transcription factor AtTCP24. The ABAP1-AtTCP24 complex bound specifically to the promoters of AtCDT1a and AtCDT1b in vitro and in vivo. Moreover, expression levels of AtCDT1a and AtCDT1b were reduced in ABAP1-overexpressing plants and they were increased in plants with reduced levels of ABAP1. We propose that ABAP1 participates in a negative feedback loop regulating mitotic DNA replication during leaf development, either by repressing transcription of pre-RC genes and possibly by regulating pre-RC utilization through direct association with pre-RC components.
Keywords
SEED DEVELOPMENT, REPEAT PROTEINS, CELL-CYCLE, GENE-EXPRESSION, ARABIDOPSIS-THALIANA, ORIGIN RECOGNITION COMPLEX, TCP, pre-RC, plant development, DNA replication, gene transcription, BINDING, PROLIFERATION, FAMILY, TCP DOMAIN

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Citation

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Chicago
Masuda, Hana Paula, Luiz Mors Cabral, Lieven De Veylder, Milos Tanurdzic, Janice de Almeida Engler, Danny Geelen, Dirk Inzé, Robert A Martienssen, Paulo CG Ferreira, and Adriana S Hemerly. 2008. “ABAP1 Is a Novel Plant Armadillo BTB Protein Involved in DNA Replication and Transcription.” Embo Journal 27 (20): 2746–2756.
APA
Masuda, H. P., Cabral, L. M., De Veylder, L., Tanurdzic, M., de Almeida Engler, J., Geelen, D., Inzé, D., et al. (2008). ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription. EMBO JOURNAL, 27(20), 2746–2756.
Vancouver
1.
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, et al. ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription. EMBO JOURNAL. 2008;27(20):2746–56.
MLA
Masuda, Hana Paula, Luiz Mors Cabral, Lieven De Veylder, et al. “ABAP1 Is a Novel Plant Armadillo BTB Protein Involved in DNA Replication and Transcription.” EMBO JOURNAL 27.20 (2008): 2746–2756. Print.
@article{445497,
  abstract     = {In multicellular organisms, organogenesis requires a tight control of the balance between cell division and cell differentiation. Distinct signalling pathways that connect both cellular processes with developmental cues might have evolved to suit different developmental plans. Here, we identified and characterized a novel protein that interacts with pre-replication complex (pre-RC) subunits, designated Armadillo BTB Arabidopsis protein 1 (ABAP1). Overexpression of ABAP1 in plants limited mitotic DNA replication and decreased cell proliferation in leaves, whereas ABAP1 downregulation increased cell division rates. Activity of ABAP1 in transcription was supported by its association with the transcription factor AtTCP24. The ABAP1-AtTCP24 complex bound specifically to the promoters of AtCDT1a and AtCDT1b in vitro and in vivo. Moreover, expression levels of AtCDT1a and AtCDT1b were reduced in ABAP1-overexpressing plants and they were increased in plants with reduced levels of ABAP1. We propose that ABAP1 participates in a negative feedback loop regulating mitotic DNA replication during leaf development, either by repressing transcription of pre-RC genes and possibly by regulating pre-RC utilization through direct association with pre-RC components.},
  author       = {Masuda, Hana Paula and Cabral, Luiz Mors and De Veylder, Lieven and Tanurdzic, Milos and de Almeida Engler, Janice and Geelen, Danny and Inz{\'e}, Dirk and Martienssen, Robert A and Ferreira, Paulo CG and Hemerly, Adriana S},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keyword      = {SEED DEVELOPMENT,REPEAT PROTEINS,CELL-CYCLE,GENE-EXPRESSION,ARABIDOPSIS-THALIANA,ORIGIN RECOGNITION COMPLEX,TCP,pre-RC,plant development,DNA replication,gene transcription,BINDING,PROLIFERATION,FAMILY,TCP DOMAIN},
  language     = {eng},
  number       = {20},
  pages        = {2746--2756},
  title        = {ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription},
  url          = {http://dx.doi.org/10.1038/emboj.2008.191},
  volume       = {27},
  year         = {2008},
}

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