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Flavonoids redirect PIN-mediated polar auxin fluxes during root gravitropic responses

Diana Santelia, Sina Henrichs, Vincent Vincenzetti, Michael Sauer, Laurent Bigler, Markus Klein, Aurélien Bailly, Youngsook Lee, Jiri Friml UGent and Markus Geisler, et al. (2008) JOURNAL OF BIOLOGICAL CHEMISTRY. 283(45). p.31218-31226
abstract
The rate, polarity, and symmetry of the flow of the plant hormone auxin are determined by the polar cellular localization of PIN-FORMED (PIN) auxin efflux carriers. Flavonoids, a class of secondary plant metabolites, have been suspected to modulate auxin transport and tropic responses. Nevertheless, the identity of specific flavonoid compounds involved and their molecular function and targets in vivo are essentially unknown. Here we show that the root elongation zone of agravitropic pin2/eir1/wav6/agr1 has an altered pattern and amount of flavonol glycosides. Application of nanomolar concentrations of flavonols to pin2 roots is sufficient to partially restore root gravitropism. By employing a quantitative cell biological approach, we demonstrate that flavonoids partially restore the formation of lateral auxin gradients in the absence of PIN2. Chemical complementation by flavonoids correlates with an asymmetric distribution of the PIN1 protein. pin2 complementation probably does not result from inhibition of auxin efflux, as supply of the auxin transport inhibitor N-1-naphthylphthalamic acid failed to restore pin2 gravitropism. We propose that flavonoids promote asymmetric PIN shifts during gravity stimulation, thus redirecting basipetal auxin streams necessary for root bending.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
P-GLYCOPROTEINS, ARABIDOPSIS-THALIANA, ABC TRANSPORTERS, EFFLUX, REGULATORS, LOCALIZATION, PROTEINS, PLANTS, ACCUMULATION, GLYCOSIDES
journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
J. Biol. Chem.
volume
283
issue
45
pages
31218 - 31226
Web of Science type
Article
Web of Science id
000260544800075
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
5.52 (2008)
JCR rank
47/276 (2008)
JCR quartile
1 (2008)
ISSN
0021-9258
DOI
10.1074/jbc.M710122200
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
445484
handle
http://hdl.handle.net/1854/LU-445484
date created
2008-12-24 02:17:00
date last changed
2012-12-06 15:17:54
@article{445484,
  abstract     = {The rate, polarity, and symmetry of the flow of the plant hormone auxin are determined by the polar cellular localization of PIN-FORMED (PIN) auxin efflux carriers. Flavonoids, a class of secondary plant metabolites, have been suspected to modulate auxin transport and tropic responses. Nevertheless, the identity of specific flavonoid compounds involved and their molecular function and targets in vivo are essentially unknown. Here we show that the root elongation zone of agravitropic pin2/eir1/wav6/agr1 has an altered pattern and amount of flavonol glycosides. Application of nanomolar concentrations of flavonols to pin2 roots is sufficient to partially restore root gravitropism. By employing a quantitative cell biological approach, we demonstrate that flavonoids partially restore the formation of lateral auxin gradients in the absence of PIN2. Chemical complementation by flavonoids correlates with an asymmetric distribution of the PIN1 protein. pin2 complementation probably does not result from inhibition of auxin efflux, as supply of the auxin transport inhibitor N-1-naphthylphthalamic acid failed to restore pin2 gravitropism. We propose that flavonoids promote asymmetric PIN shifts during gravity stimulation, thus redirecting basipetal auxin streams necessary for root bending.},
  author       = {Santelia, Diana and Henrichs, Sina and Vincenzetti, Vincent and Sauer, Michael and Bigler, Laurent and Klein, Markus and Bailly, Aur{\'e}lien and Lee, Youngsook and Friml, Jiri and Geisler, Markus and Martinoia, Enrico},
  issn         = {0021-9258},
  journal      = {JOURNAL OF BIOLOGICAL CHEMISTRY},
  keyword      = {P-GLYCOPROTEINS,ARABIDOPSIS-THALIANA,ABC TRANSPORTERS,EFFLUX,REGULATORS,LOCALIZATION,PROTEINS,PLANTS,ACCUMULATION,GLYCOSIDES},
  language     = {eng},
  number       = {45},
  pages        = {31218--31226},
  title        = {Flavonoids redirect PIN-mediated polar auxin fluxes during root gravitropic responses},
  url          = {http://dx.doi.org/10.1074/jbc.M710122200},
  volume       = {283},
  year         = {2008},
}

Chicago
Santelia, Diana, Sina Henrichs, Vincent Vincenzetti, Michael Sauer, Laurent Bigler, Markus Klein, Aurélien Bailly, et al. 2008. “Flavonoids Redirect PIN-mediated Polar Auxin Fluxes During Root Gravitropic Responses.” Journal of Biological Chemistry 283 (45): 31218–31226.
APA
Santelia, D., Henrichs, S., Vincenzetti, V., Sauer, M., Bigler, L., Klein, M., Bailly, A., et al. (2008). Flavonoids redirect PIN-mediated polar auxin fluxes during root gravitropic responses. JOURNAL OF BIOLOGICAL CHEMISTRY, 283(45), 31218–31226.
Vancouver
1.
Santelia D, Henrichs S, Vincenzetti V, Sauer M, Bigler L, Klein M, et al. Flavonoids redirect PIN-mediated polar auxin fluxes during root gravitropic responses. JOURNAL OF BIOLOGICAL CHEMISTRY. 2008;283(45):31218–26.
MLA
Santelia, Diana, Sina Henrichs, Vincent Vincenzetti, et al. “Flavonoids Redirect PIN-mediated Polar Auxin Fluxes During Root Gravitropic Responses.” JOURNAL OF BIOLOGICAL CHEMISTRY 283.45 (2008): 31218–31226. Print.