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Eubacterium limosum activates isoxanthohumol from hops (Humulus lupulus L.) into the potent phytoestrogen 8-prenylnaringenin in vitro and in rat intestine

Sam Possemiers UGent, Sylvie RABOT, Juan carlos ESPIN, Aurelia BRUNEAU, Catherine PHILIPPE, Antonio GONZALEZ-SARRIAS, Arne Heyerick UGent, Francisco TOMAS-BARBERAN, Denis De Keukeleire UGent and Willy Verstraete UGent (2008) JOURNAL OF NUTRITION. 138(7). p.1310-1316
abstract
Recently, it was shown that the exposure to the potent hop phytoestrogen 8-prenylnaringenin (8-PN) depends on intestinal bacterial activation of isoxanthohumol (IX), but this occurs in only one-third of tested individuals. As the butyrate-producing Eubacterium limosum can produce 8-PN from IX, a probiotic strategy was applied to investigate whether 8-PN production could be increased in low 8-PN producers, thus balancing phytoestrogen exposure. Using fecal samples from high (Hop +) and low (Hop -) 8-PN-producing individuals, a Hop + and Hop - dynamic intestinal model was developed. In parallel, Hop + and Hop - human microbiota-associated rats were developed, germ-free (GF) rats acting as negative controls. IX and then IX + E. limosum were administered in the intestinal model and to the rats, and changes in 8-PN production and exposure were assessed. After dosing IX, 80% was converted into 8-PN in the Hop + model and highest 8-PN production, plasma concentrations, and urinary and fecal excretion occurred in the Hop + rats. Administration of the bacterium triggered 8-PN production in the GF rats and increased 8-PN production in the Hop - model and Hop - rats. 8-PN excretion was similar in the feces (294.1 +/- 132.2 nmol/d) and urine (8.5 +/- 1.1 nmol/d) of all rats (n = 18). In addition, butyrate production increased in all rats. In conclusion, intestinal microbiota determined 8-PN production and exposure after IX intake. Moreover, E. limosum administration increased 8-PN production in low producers, resulting in similar 8-PN production in all rats.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
JOURNAL OF NUTRITION
J. Nutr.
volume
138
issue
7
pages
1310-1316 pages
Web of Science type
Article
Web of Science id
000257347400009
JCR category
NUTRITION & DIETETICS
JCR impact factor
3.647 (2008)
JCR rank
8/59 (2008)
JCR quartile
1 (2008)
ISSN
0022-3166
language
English
UGent publication?
yes
classification
A1
id
445091
handle
http://hdl.handle.net/1854/LU-445091
date created
2008-12-18 22:10:00
date last changed
2010-03-12 14:41:52
@article{445091,
  abstract     = {Recently, it was shown that the exposure to the potent hop phytoestrogen 8-prenylnaringenin (8-PN) depends on intestinal bacterial activation of isoxanthohumol (IX), but this occurs in only one-third of tested individuals. As the butyrate-producing Eubacterium limosum can produce 8-PN from IX, a probiotic strategy was applied to investigate whether 8-PN production could be increased in low 8-PN producers, thus balancing phytoestrogen exposure. Using fecal samples from high (Hop +) and low (Hop -) 8-PN-producing individuals, a Hop + and Hop - dynamic intestinal model was developed. In parallel, Hop + and Hop - human microbiota-associated rats were developed, germ-free (GF) rats acting as negative controls. IX and then IX + E. limosum were administered in the intestinal model and to the rats, and changes in 8-PN production and exposure were assessed. After dosing IX, 80\% was converted into 8-PN in the Hop + model and highest 8-PN production, plasma concentrations, and urinary and fecal excretion occurred in the Hop + rats. Administration of the bacterium triggered 8-PN production in the GF rats and increased 8-PN production in the Hop - model and Hop - rats. 8-PN excretion was similar in the feces (294.1 +/- 132.2 nmol/d) and urine (8.5 +/- 1.1 nmol/d) of all rats (n = 18). In addition, butyrate production increased in all rats. In conclusion, intestinal microbiota determined 8-PN production and exposure after IX intake. Moreover, E. limosum administration increased 8-PN production in low producers, resulting in similar 8-PN production in all rats.},
  author       = {Possemiers, Sam and RABOT, Sylvie and ESPIN, Juan carlos and BRUNEAU, Aurelia and PHILIPPE, Catherine and GONZALEZ-SARRIAS, Antonio and Heyerick, Arne and TOMAS-BARBERAN, Francisco and De Keukeleire, Denis and Verstraete, Willy},
  issn         = {0022-3166},
  journal      = {JOURNAL OF NUTRITION},
  language     = {eng},
  number       = {7},
  pages        = {1310--1316},
  title        = {Eubacterium limosum activates isoxanthohumol from hops (Humulus lupulus L.) into the potent phytoestrogen 8-prenylnaringenin in vitro and in rat intestine},
  volume       = {138},
  year         = {2008},
}

Chicago
Possemiers, Sam, Sylvie RABOT, Juan carlos ESPIN, Aurelia BRUNEAU, Catherine PHILIPPE, Antonio GONZALEZ-SARRIAS, Arne Heyerick, Francisco TOMAS-BARBERAN, Denis De Keukeleire, and Willy Verstraete. 2008. “Eubacterium Limosum Activates Isoxanthohumol from Hops (Humulus Lupulus L.) into the Potent Phytoestrogen 8-prenylnaringenin in Vitro and in Rat Intestine.” Journal of Nutrition 138 (7): 1310–1316.
APA
Possemiers, S., RABOT, S., ESPIN, J. carlos, BRUNEAU, A., PHILIPPE, C., GONZALEZ-SARRIAS, A., Heyerick, A., et al. (2008). Eubacterium limosum activates isoxanthohumol from hops (Humulus lupulus L.) into the potent phytoestrogen 8-prenylnaringenin in vitro and in rat intestine. JOURNAL OF NUTRITION, 138(7), 1310–1316.
Vancouver
1.
Possemiers S, RABOT S, ESPIN J carlos, BRUNEAU A, PHILIPPE C, GONZALEZ-SARRIAS A, et al. Eubacterium limosum activates isoxanthohumol from hops (Humulus lupulus L.) into the potent phytoestrogen 8-prenylnaringenin in vitro and in rat intestine. JOURNAL OF NUTRITION. 2008;138(7):1310–6.
MLA
Possemiers, Sam, Sylvie RABOT, Juan carlos ESPIN, et al. “Eubacterium Limosum Activates Isoxanthohumol from Hops (Humulus Lupulus L.) into the Potent Phytoestrogen 8-prenylnaringenin in Vitro and in Rat Intestine.” JOURNAL OF NUTRITION 138.7 (2008): 1310–1316. Print.