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Surface-expressed viral proteins in feline infectious peritonitis virus-infected monocytes are internalized through a clathrin- and caveolae-independent pathway

Hannah Dewerchin UGent, Els Cornelissen UGent, Evelien Van Hamme UGent, Kaatje Smits, Bruno Verhasselt UGent and Hans Nauwynck UGent (2008) JOURNAL OF GENERAL VIROLOGY. 89(11). p.2731-2740
abstract
Infection with feline infectious peritonitis virus (FIPV), a feline coronavirus, frequently leads to death in spite of a strong humoral immune response. In previous work, we reported that infected monocytes, the in vivo target cells of FIPV, express viral proteins in their plasma membranes. These proteins are quickly internalized upon binding of antibodies. As the cell surface is cleared from viral proteins, internalization might offer protection against anti body-dependent cell lysis. Here, the internalization and subsequent trafficking of the antigen-anti body complexes were characterized using biochemical, cell biological and genetic approaches. Internalization occurred through a clathrin- and caveolae-independent pathway that did not require dynamin, rafts, actin or rho-GTPases. These findings indicate that the viral antigen-anti body complexes were not internalized through any of the previously described pathways. Further characterization showed that this internalization process was independent from phosphatases and tyrosine kinases but did depend on serine/threonine kinases. After internalization, the viral antigen-antibody complexes passed through the early endosomes, where they resided only briefly, and accumulated in the late endosomes. Between 30 and 60 min after antibody addition, the complexes left the late endosomes but were not degraded in the lysosomes. This study reveals what is probably a new internalization pathway into primary monocytes, confirming once more the complexity of endocytic processes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
journal title
JOURNAL OF GENERAL VIROLOGY
J. Gen. Virol.
volume
89
issue
11
pages
2731-2740 pages
Web of Science type
Article
Web of Science id
000260885500010
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
3.092 (2008)
JCR rank
36/144 (2008)
JCR quartile
1 (2008)
ISSN
0022-1317
DOI
10.1099/vir.0.2008/002212-0
language
English
UGent publication?
yes
classification
A1
id
444807
handle
http://hdl.handle.net/1854/LU-444807
date created
2008-12-15 00:36:00
date last changed
2009-04-20 16:22:50
@article{444807,
  abstract     = {Infection with feline infectious peritonitis virus (FIPV), a feline coronavirus, frequently leads to death in spite of a strong humoral immune response. In previous work, we reported that infected monocytes, the in vivo target cells of FIPV, express viral proteins in their plasma membranes. These proteins are quickly internalized upon binding of antibodies. As the cell surface is cleared from viral proteins, internalization might offer protection against anti body-dependent cell lysis. Here, the internalization and subsequent trafficking of the antigen-anti body complexes were characterized using biochemical, cell biological and genetic approaches. Internalization occurred through a clathrin- and caveolae-independent pathway that did not require dynamin, rafts, actin or rho-GTPases. These findings indicate that the viral antigen-anti body complexes were not internalized through any of the previously described pathways. Further characterization showed that this internalization process was independent from phosphatases and tyrosine kinases but did depend on serine/threonine kinases. After internalization, the viral antigen-antibody complexes passed through the early endosomes, where they resided only briefly, and accumulated in the late endosomes. Between 30 and 60 min after antibody addition, the complexes left the late endosomes but were not degraded in the lysosomes. This study reveals what is probably a new internalization pathway into primary monocytes, confirming once more the complexity of endocytic processes.},
  author       = {Dewerchin, Hannah and Cornelissen, Els and Van Hamme, Evelien and Smits, Kaatje and Verhasselt, Bruno and Nauwynck, Hans},
  issn         = {0022-1317},
  journal      = {JOURNAL OF GENERAL VIROLOGY},
  language     = {eng},
  number       = {11},
  pages        = {2731--2740},
  title        = {Surface-expressed viral proteins in feline infectious peritonitis virus-infected monocytes are internalized through a clathrin- and caveolae-independent pathway},
  url          = {http://dx.doi.org/10.1099/vir.0.2008/002212-0},
  volume       = {89},
  year         = {2008},
}

Chicago
Dewerchin, Hannah, Els Cornelissen, Evelien Van Hamme, Kaatje Smits, Bruno Verhasselt, and Hans Nauwynck. 2008. “Surface-expressed Viral Proteins in Feline Infectious Peritonitis Virus-infected Monocytes Are Internalized Through a Clathrin- and Caveolae-independent Pathway.” Journal of General Virology 89 (11): 2731–2740.
APA
Dewerchin, H., Cornelissen, E., Van Hamme, E., Smits, K., Verhasselt, B., & Nauwynck, H. (2008). Surface-expressed viral proteins in feline infectious peritonitis virus-infected monocytes are internalized through a clathrin- and caveolae-independent pathway. JOURNAL OF GENERAL VIROLOGY, 89(11), 2731–2740.
Vancouver
1.
Dewerchin H, Cornelissen E, Van Hamme E, Smits K, Verhasselt B, Nauwynck H. Surface-expressed viral proteins in feline infectious peritonitis virus-infected monocytes are internalized through a clathrin- and caveolae-independent pathway. JOURNAL OF GENERAL VIROLOGY. 2008;89(11):2731–40.
MLA
Dewerchin, Hannah, Els Cornelissen, Evelien Van Hamme, et al. “Surface-expressed Viral Proteins in Feline Infectious Peritonitis Virus-infected Monocytes Are Internalized Through a Clathrin- and Caveolae-independent Pathway.” JOURNAL OF GENERAL VIROLOGY 89.11 (2008): 2731–2740. Print.