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A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis

Céline Sorin, Marie Declerck, Aurélie Christ, Thomas Blein, Linnan Ma, Christine Lelandais-Brière, Maria Njo UGent, Tom Beeckman UGent, Martin Crespi and Caroline Hartmann (2014) NEW PHYTOLOGIST. 202(4). p.1197-1211
abstract
In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
TRANSCRIPTION FACTORS, MEDICAGO-TRUNCATULA, GENE-EXPRESSION, root branching, Nuclear Factor Y subunit A (NF-YA), miR169 isoforms, mimicry, Arabidopsis, AUXIN, BINDING, IDENTIFICATION, THALIANA, MICRORNAS, SMALL RNAS, CCAAT PROMOTERS
journal title
NEW PHYTOLOGIST
New Phytol.
volume
202
issue
4
pages
1197 - 1211
Web of Science type
Article
Web of Science id
000335470200012
JCR category
PLANT SCIENCES
JCR impact factor
7.672 (2014)
JCR rank
6/204 (2014)
JCR quartile
1 (2014)
ISSN
0028-646X
DOI
10.1111/nph.12735
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4443736
handle
http://hdl.handle.net/1854/LU-4443736
date created
2014-07-01 13:17:11
date last changed
2016-12-19 15:39:29
@article{4443736,
  abstract     = {In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis.},
  author       = {Sorin, C{\'e}line and Declerck, Marie and Christ, Aur{\'e}lie and Blein, Thomas and Ma, Linnan and Lelandais-Bri{\`e}re, Christine and Njo, Maria and Beeckman, Tom and Crespi, Martin and Hartmann, Caroline},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  keyword      = {TRANSCRIPTION FACTORS,MEDICAGO-TRUNCATULA,GENE-EXPRESSION,root branching,Nuclear Factor Y subunit A (NF-YA),miR169 isoforms,mimicry,Arabidopsis,AUXIN,BINDING,IDENTIFICATION,THALIANA,MICRORNAS,SMALL RNAS,CCAAT PROMOTERS},
  language     = {eng},
  number       = {4},
  pages        = {1197--1211},
  title        = {A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis},
  url          = {http://dx.doi.org/10.1111/nph.12735},
  volume       = {202},
  year         = {2014},
}

Chicago
Sorin, Céline, Marie Declerck, Aurélie Christ, Thomas Blein, Linnan Ma, Christine Lelandais-Brière, Maria Njo, Tom Beeckman, Martin Crespi, and Caroline Hartmann. 2014. “A miR169 Isoform Regulates Specific NF-YA Targets and Root Architecture in Arabidopsis.” New Phytologist 202 (4): 1197–1211.
APA
Sorin, C., Declerck, M., Christ, A., Blein, T., Ma, L., Lelandais-Brière, C., Njo, M., et al. (2014). A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis. NEW PHYTOLOGIST, 202(4), 1197–1211.
Vancouver
1.
Sorin C, Declerck M, Christ A, Blein T, Ma L, Lelandais-Brière C, et al. A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis. NEW PHYTOLOGIST. 2014;202(4):1197–211.
MLA
Sorin, Céline, Marie Declerck, Aurélie Christ, et al. “A miR169 Isoform Regulates Specific NF-YA Targets and Root Architecture in Arabidopsis.” NEW PHYTOLOGIST 202.4 (2014): 1197–1211. Print.