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A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis

Céline Sorin, Marie Declerck, Aurélie Christ, Thomas Blein, Linnan Ma, Christine Lelandais-Brière, Maria Njo (UGent) , Tom Beeckman (UGent) , Martin Crespi and Caroline Hartmann
(2014) NEW PHYTOLOGIST. 202(4). p.1197-1211
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Abstract
In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis.
Keywords
TRANSCRIPTION FACTORS, MEDICAGO-TRUNCATULA, GENE-EXPRESSION, root branching, Nuclear Factor Y subunit A (NF-YA), miR169 isoforms, mimicry, Arabidopsis, AUXIN, BINDING, IDENTIFICATION, THALIANA, MICRORNAS, SMALL RNAS, CCAAT PROMOTERS

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MLA
Sorin, Céline, et al. “A MiR169 Isoform Regulates Specific NF-YA Targets and Root Architecture in Arabidopsis.” NEW PHYTOLOGIST, vol. 202, no. 4, 2014, pp. 1197–211, doi:10.1111/nph.12735.
APA
Sorin, C., Declerck, M., Christ, A., Blein, T., Ma, L., Lelandais-Brière, C., … Hartmann, C. (2014). A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis. NEW PHYTOLOGIST, 202(4), 1197–1211. https://doi.org/10.1111/nph.12735
Chicago author-date
Sorin, Céline, Marie Declerck, Aurélie Christ, Thomas Blein, Linnan Ma, Christine Lelandais-Brière, Maria Njo, Tom Beeckman, Martin Crespi, and Caroline Hartmann. 2014. “A MiR169 Isoform Regulates Specific NF-YA Targets and Root Architecture in Arabidopsis.” NEW PHYTOLOGIST 202 (4): 1197–1211. https://doi.org/10.1111/nph.12735.
Chicago author-date (all authors)
Sorin, Céline, Marie Declerck, Aurélie Christ, Thomas Blein, Linnan Ma, Christine Lelandais-Brière, Maria Njo, Tom Beeckman, Martin Crespi, and Caroline Hartmann. 2014. “A MiR169 Isoform Regulates Specific NF-YA Targets and Root Architecture in Arabidopsis.” NEW PHYTOLOGIST 202 (4): 1197–1211. doi:10.1111/nph.12735.
Vancouver
1.
Sorin C, Declerck M, Christ A, Blein T, Ma L, Lelandais-Brière C, et al. A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis. NEW PHYTOLOGIST. 2014;202(4):1197–211.
IEEE
[1]
C. Sorin et al., “A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis,” NEW PHYTOLOGIST, vol. 202, no. 4, pp. 1197–1211, 2014.
@article{4443736,
  abstract     = {{In plants, roots are essential for water and nutrient acquisition. MicroRNAs (miRNAs) regulate their target mRNAs by transcript cleavage and/or inhibition of protein translation and are known as major post-transcriptional regulators of various developmental pathways and stress responses. In Arabidopsis thaliana, four isoforms of miR169 are encoded by 14 different genes and target diverse mRNAs, encoding subunits A of the NF-Y transcription factor complex. These miRNA isoforms and their targets have previously been linked to nutrient signalling in plants. By using mimicry constructs against different isoforms of miR169 and miR-resistant versions of NF-YA genes we analysed the role of specific miR169 isoforms in root growth and branching. We identified a regulatory node involving the particular miR169defg isoform and NF-YA2 and NF-YA10 genes that acts in the control of primary root growth. The specific expression of MIM169defg constructs altered specific cell type numbers and dimensions in the root meristem. Preventing miR169defg-regulation of NF-YA2 indirectly affected laterial root initiation. We also showed that the miR169defg isoform affects NF-YA2 transcripts both at mRNA stability and translation levels. We propose that a specific miR169 isoform and the NF-YA2 target control root architecture in Arabidopsis.}},
  author       = {{Sorin, Céline and Declerck, Marie and Christ, Aurélie and Blein, Thomas and Ma, Linnan and Lelandais-Brière, Christine and Njo, Maria and Beeckman, Tom and Crespi, Martin and Hartmann, Caroline}},
  issn         = {{0028-646X}},
  journal      = {{NEW PHYTOLOGIST}},
  keywords     = {{TRANSCRIPTION FACTORS,MEDICAGO-TRUNCATULA,GENE-EXPRESSION,root branching,Nuclear Factor Y subunit A (NF-YA),miR169 isoforms,mimicry,Arabidopsis,AUXIN,BINDING,IDENTIFICATION,THALIANA,MICRORNAS,SMALL RNAS,CCAAT PROMOTERS}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{1197--1211}},
  title        = {{A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis}},
  url          = {{http://doi.org/10.1111/nph.12735}},
  volume       = {{202}},
  year         = {{2014}},
}
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