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Transcriptional coordination between leaf cell differentiation and chloroplast development established by TCP20 and the subgroup Ib bHLH transcription factors

Megan Andriankaja, Selahattin Danisman, Lorin Mignolet, Hannes Claeys, Irina Kochanke, Mattias Vermeersch UGent, Liesbeth De Milde UGent, Stefanie De Bodt UGent, Veronique Storme UGent, Aleksandra Skirycz UGent, et al. (2014) PLANT MOLECULAR BIOLOGY. 85(3). p.233-245
abstract
The establishment of the photosynthetic apparatus during chloroplast development creates a high demand for iron as a redox metal. However, iron in too high quantities becomes toxic to the plant, thus plants have evolved a complex network of iron uptake and regulation mechanisms. Here, we examined whether four of the subgroup Ib basic helix-loop-helix transcription factors (bHLH38, bHLH39, bHLH100, bHLH101), previously implicated in iron homeostasis in roots, also play a role in regulating iron metabolism in developing leaves. These transcription factor genes were strongly up-regulated during the transition from cell proliferation to expansion, and thus sink-source transition, in young developing leaves of Arabidopsis thaliana. The four subgroup Ib bHLH genes also showed reduced expression levels in developing leaves of plants treated with norflurazon, indicating their expression was tightly linked to the onset of photosynthetic activity in young leaves. In addition, we provide evidence for a mechanism whereby the transcriptional regulators SAC51 and TCP20 antagonistically regulate the expression of these four subgroup Ib bHLH genes. A loss-of-function mutant analysis also revealed that single mutants of bHLH38, bHLH39, bHLH100, and bHLH101 developed smaller rosettes than wild-type plants in soil. When grown in agar plates with reduced iron concentration, triple bhlh39 bhlh100 bhlh101 mutant plants were smaller than wild-type plants. However, measurements of the iron content in single and multiple subgroup Ib bHLH genes, as well as transcript profiling of iron response genes during early leaf development, do not support a role for bHLH38, bHLH39, bHLH100, and bHLH101 in iron homeostasis during early leaf development.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ARABIDOPSIS-THALIANA, PLANT-GROWTH, RON-DEFICIENCY RESPONSES, Basic helix-loop-helix transcription factors, Gene regulation, Iron metabolism, Arabidopsis thaliana, Leaf development, GENE-EXPRESSION, CHROMATIN IMMUNOPRECIPITATION, METAL TRANSPORTER, OXIDATIVE STRESS, CIRCADIAN CLOCK, PALE CRESS, IN-VIVO
journal title
PLANT MOLECULAR BIOLOGY
Plant Mol.Biol.
volume
85
issue
3
pages
233 - 245
Web of Science type
Article
Web of Science id
000336030800003
JCR category
PLANT SCIENCES
JCR impact factor
4.257 (2014)
JCR rank
17/204 (2014)
JCR quartile
1 (2014)
ISSN
0167-4412
DOI
10.1007/s11103-014-0180-2
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4443560
handle
http://hdl.handle.net/1854/LU-4443560
date created
2014-07-01 11:04:39
date last changed
2016-12-19 15:39:29
@article{4443560,
  abstract     = {The establishment of the photosynthetic apparatus during chloroplast development creates a high demand for iron as a redox metal. However, iron in too high quantities becomes toxic to the plant, thus plants have evolved a complex network of iron uptake and regulation mechanisms. Here, we examined whether four of the subgroup Ib basic helix-loop-helix transcription factors (bHLH38, bHLH39, bHLH100, bHLH101), previously implicated in iron homeostasis in roots, also play a role in regulating iron metabolism in developing leaves. These transcription factor genes were strongly up-regulated during the transition from cell proliferation to expansion, and thus sink-source transition, in young developing leaves of Arabidopsis thaliana. The four subgroup Ib bHLH genes also showed reduced expression levels in developing leaves of plants treated with norflurazon, indicating their expression was tightly linked to the onset of photosynthetic activity in young leaves. In addition, we provide evidence for a mechanism whereby the transcriptional regulators SAC51 and TCP20 antagonistically regulate the expression of these four subgroup Ib bHLH genes. A loss-of-function mutant analysis also revealed that single mutants of bHLH38, bHLH39, bHLH100, and bHLH101 developed smaller rosettes than wild-type plants in soil. When grown in agar plates with reduced iron concentration, triple bhlh39 bhlh100 bhlh101 mutant plants were smaller than wild-type plants. However, measurements of the iron content in single and multiple subgroup Ib bHLH genes, as well as transcript profiling of iron response genes during early leaf development, do not support a role for bHLH38, bHLH39, bHLH100, and bHLH101 in iron homeostasis during early leaf development.},
  author       = {Andriankaja, Megan and Danisman, Selahattin and Mignolet, Lorin and Claeys, Hannes and Kochanke, Irina and Vermeersch, Mattias and De Milde, Liesbeth and De Bodt, Stefanie and Storme, Veronique and Skirycz, Aleksandra and Maurer, Felix and Bauer, Petra and M{\"u}hlenbock, Per and Van Breusegem, Frank and Angenent, Gerco C and Immink, Richard GH and Inz{\'e}, Dirk},
  issn         = {0167-4412},
  journal      = {PLANT MOLECULAR BIOLOGY},
  keyword      = {ARABIDOPSIS-THALIANA,PLANT-GROWTH,RON-DEFICIENCY RESPONSES,Basic helix-loop-helix transcription factors,Gene regulation,Iron metabolism,Arabidopsis thaliana,Leaf development,GENE-EXPRESSION,CHROMATIN IMMUNOPRECIPITATION,METAL TRANSPORTER,OXIDATIVE STRESS,CIRCADIAN CLOCK,PALE CRESS,IN-VIVO},
  language     = {eng},
  number       = {3},
  pages        = {233--245},
  title        = {Transcriptional coordination between leaf cell differentiation and chloroplast development established by TCP20 and the subgroup Ib bHLH transcription factors},
  url          = {http://dx.doi.org/10.1007/s11103-014-0180-2},
  volume       = {85},
  year         = {2014},
}

Chicago
Andriankaja, Megan, Selahattin Danisman, Lorin Mignolet, Hannes Claeys, Irina Kochanke, Mattias Vermeersch, Liesbeth De Milde, et al. 2014. “Transcriptional Coordination Between Leaf Cell Differentiation and Chloroplast Development Established by TCP20 and the Subgroup Ib bHLH Transcription Factors.” Plant Molecular Biology 85 (3): 233–245.
APA
Andriankaja, M., Danisman, S., Mignolet, L., Claeys, H., Kochanke, I., Vermeersch, M., De Milde, L., et al. (2014). Transcriptional coordination between leaf cell differentiation and chloroplast development established by TCP20 and the subgroup Ib bHLH transcription factors. PLANT MOLECULAR BIOLOGY, 85(3), 233–245.
Vancouver
1.
Andriankaja M, Danisman S, Mignolet L, Claeys H, Kochanke I, Vermeersch M, et al. Transcriptional coordination between leaf cell differentiation and chloroplast development established by TCP20 and the subgroup Ib bHLH transcription factors. PLANT MOLECULAR BIOLOGY. 2014;85(3):233–45.
MLA
Andriankaja, Megan, Selahattin Danisman, Lorin Mignolet, et al. “Transcriptional Coordination Between Leaf Cell Differentiation and Chloroplast Development Established by TCP20 and the Subgroup Ib bHLH Transcription Factors.” PLANT MOLECULAR BIOLOGY 85.3 (2014): 233–245. Print.