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Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1)

Bart Broeckx (UGent) , Frank Coopman (UGent) , Geert Verhoeven (UGent) , Valérie Bavegems (UGent) , Sarah De Keulenaer (UGent) , Ellen De Meester (UGent) , Filip Van Nieuwerburgh (UGent) and Dieter Deforce (UGent)
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Abstract
Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86% of the target regions were completely sequenced with a minimum coverage of five and 90% of the reads mapped on the target regions. Additionally, it is shown that the reproducibility within and between captures is high and that pooling four samples per capture is a valid option. Overall, we have demonstrated the strong performance of this WES enrichment kit and are confident it will be a valuable tool in future disease association studies.
Keywords
TOOL, CAPTURE

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Chicago
Broeckx, Bart, Frank Coopman, Geert Verhoeven, Valérie Bavegems, Sarah De Keulenaer, Ellen De Meester, Filip Van Nieuwerburgh, and Dieter Deforce. 2014. “Development and Performance of a Targeted Whole Exome Sequencing Enrichment Kit for the Dog (Canis Familiaris Build 3.1).” Scientific Reports 4.
APA
Broeckx, B., Coopman, F., Verhoeven, G., Bavegems, V., De Keulenaer, S., De Meester, E., Van Nieuwerburgh, F., et al. (2014). Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1). SCIENTIFIC REPORTS, 4.
Vancouver
1.
Broeckx B, Coopman F, Verhoeven G, Bavegems V, De Keulenaer S, De Meester E, et al. Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1). SCIENTIFIC REPORTS. 2014;4.
MLA
Broeckx, Bart, Frank Coopman, Geert Verhoeven, et al. “Development and Performance of a Targeted Whole Exome Sequencing Enrichment Kit for the Dog (Canis Familiaris Build 3.1).” SCIENTIFIC REPORTS 4 (2014): n. pag. Print.
@article{4432373,
  abstract     = {Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86\% of the target regions were completely sequenced with a minimum coverage of five and 90\% of the reads mapped on the target regions. Additionally, it is shown that the reproducibility within and between captures is high and that pooling four samples per capture is a valid option. Overall, we have demonstrated the strong performance of this WES enrichment kit and are confident it will be a valuable tool in future disease association studies.},
  articleno    = {5597},
  author       = {Broeckx, Bart and Coopman, Frank and Verhoeven, Geert and Bavegems, Val{\'e}rie and De Keulenaer, Sarah and De Meester, Ellen and Van Nieuwerburgh, Filip and Deforce, Dieter},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  language     = {eng},
  pages        = {4},
  title        = {Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1)},
  url          = {http://dx.doi.org/10.1038/srep05597},
  volume       = {4},
  year         = {2014},
}

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