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Fatty acid composition of the follicular fluid of normal weight, overweight and obese women undergoing assisted reproductive treatment: a descriptive cross-sectional study

Sara DM Valckx, Maria Arias-Alvarez, Ingrid De Pauw, Veerle Fievez UGent, Bruno Vlaeminck UGent, Erik Fransen, Peter EJ Bols and Jo LMR Leroy (2014) REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY. 12.
abstract
Background: It has been well documented that the maturing oocyte is very vulnerable to changes in its micro-environment, the follicular fluid (FF). Recent research has focused on different components within this FF, like hormones, growth factors and metabolites, and how their concentrations are altered by diet and the metabolic health of the mother. It has been proposed that fatty acids (FAs) are potential factors that influence oocyte maturation and subsequent embryo development. However, a thorough study of the specific FF FA composition per lipid fraction and how this may be affected by BMI is currently lacking. Therefore, we investigated the BMI-related concentration of FAs in the phospholipid (PL), cholesteryl-ester (CHE), triglyceride (TG) and non-esterified (NE) lipid fraction in the FF of women undergoing assisted reproductive treatment (ART). Methods: In this descriptive cross-sectional study, the FF of normal weight (18.5 <= BMI < 25.0 kg/m(2), n = 10), overweight (25.0 <= BMI < 30.0 kg/m(2), n = 10) and obese (BMI >= 30.0 kg/m(2), n = 10) women, undergoing ART, was sampled and analyzed for 23 specific FAs in the PL, CHE, TG and NEFA fraction, using a gas chromatographic analysis method. Differences between BMI-groups were studied by means of univariate general linear models and post hoc Sheffe tests. Results: Total FA concentrations in the PL and CHE fraction did not differ between BMI groups. Total TG concentrations tended to differ and total NEFA concentrations differed significantly between BMI groups. Interestingly, 42% and 34% of the total FAs was esterified in the PL and CHE fraction, respectively, while only 10% were present in both the TG and NEFA fraction. Only few individual FA concentrations differed in the PL, CHE and TG fraction between BMI groups, whereas abundant BMI-related differences were found in the NEFA fraction. Conclusions: Our data show that differences in BMI are associated with alterations in the FA composition of the FF, an effect most pronounced in the NEFA fraction. These BMI-related variations could possibly affect granulosa cell viability, oocyte developmental competence and subsequent embryo quality possibly explaining differences in oocyte quality in obese patients described by others.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BMI, CUMULUS-OOCYTE COMPLEXES, Follicular fluid, NEFA, Obesity, Fatty acid, ART, LINOLEIC-ACID, GRANULOSA-CELLS, OVARIAN COMPARTMENTS, EARLY EMBRYO DEVELOPMENT, SOLID-PHASE EXTRACTION, PROLIFERATION IN-VITRO, DAIRY-COWS, CELL STEROIDOGENESIS, BOVINE-OOCYTES
journal title
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
Reprod. Biol. Endocrinol.
volume
12
article number
13
pages
11 pages
Web of Science type
Article
Web of Science id
000331638300001
JCR category
REPRODUCTIVE BIOLOGY
JCR impact factor
2.226 (2014)
JCR rank
18/30 (2014)
JCR quartile
3 (2014)
ISSN
1477-7827
DOI
10.1186/1477-7827-12-13
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
4430267
handle
http://hdl.handle.net/1854/LU-4430267
date created
2014-06-26 14:16:43
date last changed
2016-12-21 15:42:30
@article{4430267,
  abstract     = {Background: It has been well documented that the maturing oocyte is very vulnerable to changes in its micro-environment, the follicular fluid (FF). Recent research has focused on different components within this FF, like hormones, growth factors and metabolites, and how their concentrations are altered by diet and the metabolic health of the mother. It has been proposed that fatty acids (FAs) are potential factors that influence oocyte maturation and subsequent embryo development. However, a thorough study of the specific FF FA composition per lipid fraction and how this may be affected by BMI is currently lacking. Therefore, we investigated the BMI-related concentration of FAs in the phospholipid (PL), cholesteryl-ester (CHE), triglyceride (TG) and non-esterified (NE) lipid fraction in the FF of women undergoing assisted reproductive treatment (ART).
Methods: In this descriptive cross-sectional study, the FF of normal weight (18.5 {\textlangle}= BMI {\textlangle} 25.0 kg/m(2), n = 10), overweight (25.0 {\textlangle}= BMI {\textlangle} 30.0 kg/m(2), n = 10) and obese (BMI {\textrangle}= 30.0 kg/m(2), n = 10) women, undergoing ART, was sampled and analyzed for 23 specific FAs in the PL, CHE, TG and NEFA fraction, using a gas chromatographic analysis method. Differences between BMI-groups were studied by means of univariate general linear models and post hoc Sheffe tests.
Results: Total FA concentrations in the PL and CHE fraction did not differ between BMI groups. Total TG concentrations tended to differ and total NEFA concentrations differed significantly between BMI groups. Interestingly, 42\% and 34\% of the total FAs was esterified in the PL and CHE fraction, respectively, while only 10\% were present in both the TG and NEFA fraction. Only few individual FA concentrations differed in the PL, CHE and TG fraction between BMI groups, whereas abundant BMI-related differences were found in the NEFA fraction.
Conclusions: Our data show that differences in BMI are associated with alterations in the FA composition of the FF, an effect most pronounced in the NEFA fraction. These BMI-related variations could possibly affect granulosa cell viability, oocyte developmental competence and subsequent embryo quality possibly explaining differences in oocyte quality in obese patients described by others.},
  articleno    = {13},
  author       = {Valckx, Sara DM and Arias-Alvarez, Maria and De Pauw, Ingrid and Fievez, Veerle and Vlaeminck, Bruno and Fransen, Erik and Bols, Peter EJ and Leroy, Jo LMR},
  issn         = {1477-7827},
  journal      = {REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY},
  keyword      = {BMI,CUMULUS-OOCYTE COMPLEXES,Follicular fluid,NEFA,Obesity,Fatty acid,ART,LINOLEIC-ACID,GRANULOSA-CELLS,OVARIAN COMPARTMENTS,EARLY EMBRYO DEVELOPMENT,SOLID-PHASE EXTRACTION,PROLIFERATION IN-VITRO,DAIRY-COWS,CELL STEROIDOGENESIS,BOVINE-OOCYTES},
  language     = {eng},
  pages        = {11},
  title        = {Fatty acid composition of the follicular fluid of normal weight, overweight and obese women undergoing assisted reproductive treatment: a descriptive cross-sectional study},
  url          = {http://dx.doi.org/10.1186/1477-7827-12-13},
  volume       = {12},
  year         = {2014},
}

Chicago
Valckx, Sara DM, Maria Arias-Alvarez, Ingrid De Pauw, Veerle Fievez, Bruno Vlaeminck, Erik Fransen, Peter EJ Bols, and Jo LMR Leroy. 2014. “Fatty Acid Composition of the Follicular Fluid of Normal Weight, Overweight and Obese Women Undergoing Assisted Reproductive Treatment: a Descriptive Cross-sectional Study.” Reproductive Biology and Endocrinology 12.
APA
Valckx, S. D., Arias-Alvarez, M., De Pauw, I., Fievez, V., Vlaeminck, B., Fransen, E., Bols, P. E., et al. (2014). Fatty acid composition of the follicular fluid of normal weight, overweight and obese women undergoing assisted reproductive treatment: a descriptive cross-sectional study. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 12.
Vancouver
1.
Valckx SD, Arias-Alvarez M, De Pauw I, Fievez V, Vlaeminck B, Fransen E, et al. Fatty acid composition of the follicular fluid of normal weight, overweight and obese women undergoing assisted reproductive treatment: a descriptive cross-sectional study. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY. 2014;12.
MLA
Valckx, Sara DM, Maria Arias-Alvarez, Ingrid De Pauw, et al. “Fatty Acid Composition of the Follicular Fluid of Normal Weight, Overweight and Obese Women Undergoing Assisted Reproductive Treatment: a Descriptive Cross-sectional Study.” REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY 12 (2014): n. pag. Print.