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Stationary phase optimized selectivity supercritical fluid chromatography (SOS-SFC)

Sander Delahaye and Frederic Lynen UGent (2014) 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts.
abstract
In stationary phase optimized selectivity liquid chromatography (SOS-LC) the stationary phase becomes a tunable parameter by connecting column segments with variable lengths of different stationary phases. An optimization procedure and algorithm based on the PRISMA model for the optimization of the mobile phase in LC has been developed to apply this strategy for isocratic and gradient separations. An optimized column segment combination, giving the highest separation selectivity for all compounds in a mixture in the shortest possible analysis time, is predicted using the retention factors that are measured on the individual columns containing different stationary phases. The PRISMA model is only valid if the retention factor k is independent of column length and applied pressure. It’s shown to be reliable for conventional HPLC conditions, but the applicability in SFC is more difficult. The reason for this, is that in SFC, the retention factor decreases with increasing pressure. If a fixed outlet pressure is used, the column pressure increases with increasing column length, making it in theory impossible to use measured retention factors on a given column length to predict retention factors on column segments of different lengths. In previous work, it was demonstrated that it is possible to perform SFC separations on a fixed column length at different flow rates keeping the retention factors of the compounds fixed by using the isopycnic way of working. This means that the average density in the column is kept constant by varying the outlet pressure. Because this isopycnic approach allows control of k, it is worthwhile investigating the implementation of the SOS-LC model for SFC separations. In this work, stationary phase optimized SFC is performed and shown to be viable if a fixed average column pressure is used for all measurements by varying the outlet pressure.
Please use this url to cite or link to this publication:
author
organization
year
type
conference
publication status
published
subject
in
13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts
conference name
13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) ; 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3)
conference location
Brugge, Belgium
conference start
2014-01-28
conference end
2014-01-31
language
English
UGent publication?
yes
classification
C3
id
4429807
handle
http://hdl.handle.net/1854/LU-4429807
date created
2014-06-26 10:27:32
date last changed
2016-12-19 15:37:25
@inproceedings{4429807,
  abstract     = {In stationary phase optimized selectivity liquid chromatography (SOS-LC) the stationary phase becomes a tunable parameter by connecting column segments with variable lengths of different stationary phases. An optimization procedure and algorithm based on the PRISMA model for the optimization of the mobile phase in LC has been developed to apply this strategy for isocratic and gradient separations. An optimized column segment combination, giving the highest separation selectivity for all compounds in a mixture in the shortest possible analysis time, is predicted using the retention factors that are measured on the individual columns containing different stationary phases. 
The PRISMA model is only valid if the retention factor k is independent of column length and applied pressure. It{\textquoteright}s shown to be reliable for conventional HPLC conditions, but the applicability in SFC is more difficult. The reason for this, is that in SFC, the retention factor decreases with increasing pressure. If a fixed outlet pressure is used, the column pressure increases with increasing column length, making it in theory impossible to use measured retention factors on a given column length to predict retention factors on column segments of different lengths. In previous work, it was demonstrated that it is possible to perform SFC separations on a fixed column length at different flow rates keeping the retention factors of the compounds fixed by using the isopycnic way of working. This means that the average density in the column is kept constant by varying the outlet pressure. Because this isopycnic approach allows control of k, it is worthwhile investigating the implementation of the SOS-LC model for SFC separations. In this work, stationary phase optimized SFC is performed and shown to be viable if a fixed average column pressure is used for all measurements by varying the outlet pressure.},
  author       = {Delahaye, Sander and Lynen, Frederic},
  booktitle    = {13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts},
  language     = {eng},
  location     = {Brugge, Belgium},
  title        = {Stationary phase optimized selectivity supercritical fluid chromatography (SOS-SFC)},
  year         = {2014},
}

Chicago
Delahaye, Sander, and Frederic Lynen. 2014. “Stationary Phase Optimized Selectivity Supercritical Fluid Chromatography (SOS-SFC).” In 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of Abstracts.
APA
Delahaye, S., & Lynen, F. (2014). Stationary phase optimized selectivity supercritical fluid chromatography (SOS-SFC). 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts. Presented at the 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) ; 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3).
Vancouver
1.
Delahaye S, Lynen F. Stationary phase optimized selectivity supercritical fluid chromatography (SOS-SFC). 13th International symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of abstracts. 2014.
MLA
Delahaye, Sander, and Frederic Lynen. “Stationary Phase Optimized Selectivity Supercritical Fluid Chromatography (SOS-SFC).” 13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3), Book of Abstracts. 2014. Print.