Advanced search
1 file | 122.15 KB Add to list

Semi-direct lysis of swabs and evaluation of their efficiencies to recover human noroviruses GI and GII from surfaces

Ann De Keuckelaere (UGent) , Ambroos Stals (UGent) and Mieke Uyttendaele (UGent)
Author
Organization
Project
Veg-i-Trade, an European Community's Seventh framework program (grant agreement No. 244994)
Abstract
Enteric viruses such as noroviruses (NoVs) continue to be the cause of widespread viral outbreaks due to person-to-person transmission, contaminated food, and contaminated surfaces. In order to optimize swabbing methodology for the detection of viruses on (food) contact surfaces, three swab elution/extraction strategies were compared in part one of this study, out of which, one strategy was based on the recently launched ISO protocol (ISO/TS 15216-1) for the determination of hepatitis A virus and NoV in food using real-time RT-PCR (RT-qPCR). These three swab elution/extraction strategies were tested for the detection of GI.4 and GII.4 NoV on high-density polyethylene (HD-PE) surfaces with the use of cotton swabs. For detection of GI.4 and GII.4, the sample recovery efficiency (SRE) obtained with the direct lysis strategy (based on ISO/TS 15216-1) was significantly lower than the SRE obtained with both other strategies. The semi-direct lysis strategy was chosen to assess the SRE of two common swabs (cotton swab and polyester swab) versus the biowipe (Biom,rieux, Lyon, France) on three surfaces (HD-PE, neoprene rubber (NR), and nitrile gloves (GL)). For both surfaces, HD-PE and GL, no significant differences in SREs of GI.4 and GII.4 NoVs were detected between the three different swabs. For the coarser NR, biowipes turned out to be the best option for detecting both GI.4 and GII.4 NoV.
Keywords
FOOD-INDUSTRY, ENVIRONMENTAL CONTAMINATION, OUTBREAK, GASTROENTERITIS, FOMITES, VIRUSES, CALICIVIRUSES, TRANSMISSION, high-density polyethylene, neoprene rubber, real-time RT-PCR, environment, norovirus, swab, gloves, HYGIENE, TOOL

Downloads

  • Article Ann De Keuckelaere topic swabs revision 3 biblio version.docx
    • full text
    • |
    • open access
    • |
    • Word
    • |
    • 122.15 KB

Citation

Please use this url to cite or link to this publication:

MLA
De Keuckelaere, Ann, Ambroos Stals, and Mieke Uyttendaele. “Semi-direct Lysis of Swabs and Evaluation of Their Efficiencies to Recover Human Noroviruses GI and GII from Surfaces.” FOOD AND ENVIRONMENTAL VIROLOGY 6.2 (2014): 132–139. Print.
APA
De Keuckelaere, A., Stals, A., & Uyttendaele, M. (2014). Semi-direct lysis of swabs and evaluation of their efficiencies to recover human noroviruses GI and GII from surfaces. FOOD AND ENVIRONMENTAL VIROLOGY, 6(2), 132–139.
Chicago author-date
De Keuckelaere, Ann, Ambroos Stals, and Mieke Uyttendaele. 2014. “Semi-direct Lysis of Swabs and Evaluation of Their Efficiencies to Recover Human Noroviruses GI and GII from Surfaces.” Food and Environmental Virology 6 (2): 132–139.
Chicago author-date (all authors)
De Keuckelaere, Ann, Ambroos Stals, and Mieke Uyttendaele. 2014. “Semi-direct Lysis of Swabs and Evaluation of Their Efficiencies to Recover Human Noroviruses GI and GII from Surfaces.” Food and Environmental Virology 6 (2): 132–139.
Vancouver
1.
De Keuckelaere A, Stals A, Uyttendaele M. Semi-direct lysis of swabs and evaluation of their efficiencies to recover human noroviruses GI and GII from surfaces. FOOD AND ENVIRONMENTAL VIROLOGY. 2014;6(2):132–9.
IEEE
[1]
A. De Keuckelaere, A. Stals, and M. Uyttendaele, “Semi-direct lysis of swabs and evaluation of their efficiencies to recover human noroviruses GI and GII from surfaces,” FOOD AND ENVIRONMENTAL VIROLOGY, vol. 6, no. 2, pp. 132–139, 2014.
@article{4428844,
  abstract     = {Enteric viruses such as noroviruses (NoVs) continue to be the cause of widespread viral outbreaks due to person-to-person transmission, contaminated food, and contaminated surfaces. In order to optimize swabbing methodology for the detection of viruses on (food) contact surfaces, three swab elution/extraction strategies were compared in part one of this study, out of which, one strategy was based on the recently launched ISO protocol (ISO/TS 15216-1) for the determination of hepatitis A virus and NoV in food using real-time RT-PCR (RT-qPCR). These three swab elution/extraction strategies were tested for the detection of GI.4 and GII.4 NoV on high-density polyethylene (HD-PE) surfaces with the use of cotton swabs. For detection of GI.4 and GII.4, the sample recovery efficiency (SRE) obtained with the direct lysis strategy (based on ISO/TS 15216-1) was significantly lower than the SRE obtained with both other strategies. The semi-direct lysis strategy was chosen to assess the SRE of two common swabs (cotton swab and polyester swab) versus the biowipe (Biom,rieux, Lyon, France) on three surfaces (HD-PE, neoprene rubber (NR), and nitrile gloves (GL)). For both surfaces, HD-PE and GL, no significant differences in SREs of GI.4 and GII.4 NoVs were detected between the three different swabs. For the coarser NR, biowipes turned out to be the best option for detecting both GI.4 and GII.4 NoV.},
  author       = {De Keuckelaere, Ann and Stals, Ambroos and Uyttendaele, Mieke},
  issn         = {1867-0334},
  journal      = {FOOD AND ENVIRONMENTAL VIROLOGY},
  keywords     = {FOOD-INDUSTRY,ENVIRONMENTAL CONTAMINATION,OUTBREAK,GASTROENTERITIS,FOMITES,VIRUSES,CALICIVIRUSES,TRANSMISSION,high-density polyethylene,neoprene rubber,real-time RT-PCR,environment,norovirus,swab,gloves,HYGIENE,TOOL},
  language     = {eng},
  number       = {2},
  pages        = {132--139},
  title        = {Semi-direct lysis of swabs and evaluation of their efficiencies to recover human noroviruses GI and GII from surfaces},
  url          = {http://dx.doi.org/10.1007/s12560-014-9144-6},
  volume       = {6},
  year         = {2014},
}

Altmetric
View in Altmetric
Web of Science
Times cited: