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Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts

Michaël Beck, Marjan Moreels, Roel Quintens, Khalil Abou-El-Ardat, Hussein El-Saghire, Kevin Tabury, Arlette Michaux, Ann Janssen, Mieke Neefs, Patric Van Oostveldt UGent, et al. (2014) INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE. 34(2). p.606-615
abstract
Microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments. In the present study, an experimental model was utilized to monitor gene expression changes in fetal skin fibroblasts of murine origin. Cells were continuously subjected for 65 h to a low dose. (55 mSv) of ionizing radiation (IR), comprising a mixture of high-linear energy transfer (LET) neutrons and low-LET gamma-rays, and/or simulated microgravity using the random positioning machine (RPM), after which microarrays were performed. The data were analyzed both by gene set enrichment analysis (GSEA) and single gene analysis (SGA). Simulated microgravity affected fetal murine fibroblasts by inducing oxidative stress responsive genes. Three of these genes are targets of the nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), which may play a role in the cell response to simulated microgravity. In addition, simulated gravity decreased the expression of genes involved in cytoskeleton remodeling, which may have been caused by the downregulation of the serum response factor (SRF), possibly through the Rho signaling pathway. Similarly, chronic exposure to low-dose IR caused the downregulation of genes involved in cytoskeleton remodeling, as well as in cell cycle regulation and DNA damage response pathways. Many of the genes or gene sets that were altered in the individual treatments (RPM or IR) were not altered in the combined treatment (RPM and IR), indicating a complex interaction between RPM and IR.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
microarrays, simulated space conditions, cytoskeleton, oxidative stress, DNA damage, RANDOM-POSITIONING MACHINE, SET ENRICHMENT ANALYSIS, RHO-GTPASES, EXPRESSION PROFILES, GENE-EXPRESSION, MICROGRAVITY, GRAVITY, CELLS, ACTIVATION, SENESCENCE
journal title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Int. J. Mol. Med.
volume
34
issue
2
pages
606 - 615
Web of Science type
Article
Web of Science id
000339127900030
JCR category
MEDICINE, RESEARCH & EXPERIMENTAL
JCR impact factor
2.088 (2014)
JCR rank
70/123 (2014)
JCR quartile
3 (2014)
ISSN
1107-3756
DOI
10.3892/ijmm.2014.1785
project
NB-Photonics
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4426455
handle
http://hdl.handle.net/1854/LU-4426455
date created
2014-06-21 11:22:27
date last changed
2017-05-04 10:41:27
@article{4426455,
  abstract     = {Microgravity and cosmic rays as found in space are difficult to recreate on earth. However, ground-based models exist to simulate space flight experiments. In the present study, an experimental model was utilized to monitor gene expression changes in fetal skin fibroblasts of murine origin. Cells were continuously subjected for 65 h to a low dose. (55 mSv) of ionizing radiation (IR), comprising a mixture of high-linear energy transfer (LET) neutrons and low-LET gamma-rays, and/or simulated microgravity using the random positioning machine (RPM), after which microarrays were performed. The data were analyzed both by gene set enrichment analysis (GSEA) and single gene analysis (SGA). Simulated microgravity affected fetal murine fibroblasts by inducing oxidative stress responsive genes. Three of these genes are targets of the nuclear factor-erythroid 2 p45-related factor 2 (Nrf2), which may play a role in the cell response to simulated microgravity. In addition, simulated gravity decreased the expression of genes involved in cytoskeleton remodeling, which may have been caused by the downregulation of the serum response factor (SRF), possibly through the Rho signaling pathway. Similarly, chronic exposure to low-dose IR caused the downregulation of genes involved in cytoskeleton remodeling, as well as in cell cycle regulation and DNA damage response pathways. Many of the genes or gene sets that were altered in the individual treatments (RPM or IR) were not altered in the combined treatment (RPM and IR), indicating a complex interaction between RPM and IR.},
  author       = {Beck, Micha{\"e}l and Moreels, Marjan and Quintens, Roel and Abou-El-Ardat, Khalil and El-Saghire, Hussein and Tabury, Kevin and Michaux, Arlette and Janssen, Ann and Neefs, Mieke and Van Oostveldt, Patric and De Vos, Winnok and Baatout, Sarah},
  issn         = {1107-3756},
  journal      = {INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE},
  keyword      = {microarrays,simulated space conditions,cytoskeleton,oxidative stress,DNA damage,RANDOM-POSITIONING MACHINE,SET ENRICHMENT ANALYSIS,RHO-GTPASES,EXPRESSION PROFILES,GENE-EXPRESSION,MICROGRAVITY,GRAVITY,CELLS,ACTIVATION,SENESCENCE},
  language     = {eng},
  number       = {2},
  pages        = {606--615},
  title        = {Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts},
  url          = {http://dx.doi.org/10.3892/ijmm.2014.1785},
  volume       = {34},
  year         = {2014},
}

Chicago
Beck, Michaël, Marjan Moreels, Roel Quintens, Khalil Abou-El-Ardat, Hussein El-Saghire, Kevin Tabury, Arlette Michaux, et al. 2014. “Chronic Exposure to Simulated Space Conditions Predominantly Affects Cytoskeleton Remodeling and Oxidative Stress Response in Mouse Fetal Fibroblasts.” International Journal of Molecular Medicine 34 (2): 606–615.
APA
Beck, Michaël, Moreels, M., Quintens, R., Abou-El-Ardat, K., El-Saghire, H., Tabury, K., Michaux, A., et al. (2014). Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 34(2), 606–615.
Vancouver
1.
Beck M, Moreels M, Quintens R, Abou-El-Ardat K, El-Saghire H, Tabury K, et al. Chronic exposure to simulated space conditions predominantly affects cytoskeleton remodeling and oxidative stress response in mouse fetal fibroblasts. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE. 2014;34(2):606–15.
MLA
Beck, Michaël, Marjan Moreels, Roel Quintens, et al. “Chronic Exposure to Simulated Space Conditions Predominantly Affects Cytoskeleton Remodeling and Oxidative Stress Response in Mouse Fetal Fibroblasts.” INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 34.2 (2014): 606–615. Print.