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Orthogonal array design for optimization of an artificial medium for in vitro rearing of Trichogramma dendrolimi

Xin Lü, Shichou Han, Patrick De Clercq UGent, Jianqing Dai and Liying Li (2014) ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA. 152(1). p.52-60
abstract
Successful complete development of Trichogramma species on artificial media is also related to the presence and proportions of ingredients other than host insect-derived components. In this study, an orthogonal array with six factors at three levels was performed and parameters of parasitism, larval development, pupation, and adult emergence were monitored to reveal the most important components of an artificial medium and to improve the medium for in vitro mass rearing of Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae). Results indicated that biological parameters of T.dendrolimi were affected differently by six ingredients of the artificial medium: pupal hemolymph of Antheraea pernyi (Guerin-Meneville) (Lepidoptera: Saturniidae), egg yolk, 10% malted milk solution, Neisenheimer's salt solution, trehalose, and sterile water. Statistical analysis indicated that trehalose and Neisenheimer's salt solution, 10% malted milk solution, and pupal hemolymph of A.pernyi were the main ingredients of the artificial medium based on rates of parasitism and pupation, the number of larvae developing in each artificial egg, adult emergence rate, and the number of normal adults produced. A follow-up bioassay with a selection of optimized formulas confirmed the validity of the optimization as predicted by the orthogonal array analysis, indicating the usefulness of this method for selecting artificial diets for entomophagous insects. Adult emergence rate of the parasitoid and total number of normal adults produced per egg card (each containing 20 artificial eggs) averaged 88.8% and 956 females on the best performing optimized artificial medium, consisting of 3ml pupal hemolymph of A.pernyi, 2.5ml egg yolk, 1ml 10% malted milk solution, 1ml Neisenheimer's salt solution, 0.1g trehalose, and 1.5ml sterile water. The latter medium was superior to any formerly developed medium and may thus have potential for the in vitro mass rearing of T.dendrolimi.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Hymenoptera, Trichogrammatidae, trehalose, Antheraea pernyi, mass rearing, biological control, diet optimization, DIET, HYMENOPTERA, HEMOLYMPH, PRETIOSUM, INVITRO, WASP
journal title
ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA
Entomol. Exp. Appl.
volume
152
issue
1
pages
52 - 60
Web of Science type
Article
Web of Science id
000337625500006
JCR category
ENTOMOLOGY
JCR impact factor
1.616 (2014)
JCR rank
27/92 (2014)
JCR quartile
2 (2014)
ISSN
0013-8703
DOI
10.1111/eea.12194
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4425910
handle
http://hdl.handle.net/1854/LU-4425910
date created
2014-06-20 13:35:03
date last changed
2016-12-19 15:47:04
@article{4425910,
  abstract     = {Successful complete development of Trichogramma species on artificial media is also related to the presence and proportions of ingredients other than host insect-derived components. In this study, an orthogonal array with six factors at three levels was performed and parameters of parasitism, larval development, pupation, and adult emergence were monitored to reveal the most important components of an artificial medium and to improve the medium for in vitro mass rearing of Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae). Results indicated that biological parameters of T.dendrolimi were affected differently by six ingredients of the artificial medium: pupal hemolymph of Antheraea pernyi (Guerin-Meneville) (Lepidoptera: Saturniidae), egg yolk, 10\% malted milk solution, Neisenheimer's salt solution, trehalose, and sterile water. Statistical analysis indicated that trehalose and Neisenheimer's salt solution, 10\% malted milk solution, and pupal hemolymph of A.pernyi were the main ingredients of the artificial medium based on rates of parasitism and pupation, the number of larvae developing in each artificial egg, adult emergence rate, and the number of normal adults produced. A follow-up bioassay with a selection of optimized formulas confirmed the validity of the optimization as predicted by the orthogonal array analysis, indicating the usefulness of this method for selecting artificial diets for entomophagous insects. Adult emergence rate of the parasitoid and total number of normal adults produced per egg card (each containing 20 artificial eggs) averaged 88.8\% and 956 females on the best performing optimized artificial medium, consisting of 3ml pupal hemolymph of A.pernyi, 2.5ml egg yolk, 1ml 10\% malted milk solution, 1ml Neisenheimer's salt solution, 0.1g trehalose, and 1.5ml sterile water. The latter medium was superior to any formerly developed medium and may thus have potential for the in vitro mass rearing of T.dendrolimi.},
  author       = {L{\"u}, Xin and Han, Shichou and De Clercq, Patrick and Dai, Jianqing and Li, Liying},
  issn         = {0013-8703},
  journal      = {ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA},
  keyword      = {Hymenoptera,Trichogrammatidae,trehalose,Antheraea pernyi,mass rearing,biological control,diet optimization,DIET,HYMENOPTERA,HEMOLYMPH,PRETIOSUM,INVITRO,WASP},
  language     = {eng},
  number       = {1},
  pages        = {52--60},
  title        = {Orthogonal array design for optimization of an artificial medium for in vitro rearing of Trichogramma dendrolimi},
  url          = {http://dx.doi.org/10.1111/eea.12194},
  volume       = {152},
  year         = {2014},
}

Chicago
Lü, Xin, Shichou Han, Patrick De Clercq, Jianqing Dai, and Liying Li. 2014. “Orthogonal Array Design for Optimization of an Artificial Medium for in Vitro Rearing of Trichogramma Dendrolimi.” Entomologia Experimentalis Et Applicata 152 (1): 52–60.
APA
Lü, X., Han, S., De Clercq, P., Dai, J., & Li, L. (2014). Orthogonal array design for optimization of an artificial medium for in vitro rearing of Trichogramma dendrolimi. ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA, 152(1), 52–60.
Vancouver
1.
Lü X, Han S, De Clercq P, Dai J, Li L. Orthogonal array design for optimization of an artificial medium for in vitro rearing of Trichogramma dendrolimi. ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA. 2014;152(1):52–60.
MLA
Lü, Xin, Shichou Han, Patrick De Clercq, et al. “Orthogonal Array Design for Optimization of an Artificial Medium for in Vitro Rearing of Trichogramma Dendrolimi.” ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA 152.1 (2014): 52–60. Print.