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Glycated nail proteins : a new approach for detecting diabetes in developing countries

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Abstract
Objective: To assess glycation of nail proteins as a tool in the diagnosis of diabetes. Methods: Glycation of nail proteins was assessed using a modified photometric nitroblue tetrazolium-based assay, which provides information about average glucose values of the last 6-9months. Analysis is possible on 10mg of nail clippings with a within-run coefficient of variation (CV) of 11%. The analyte is extremely stable. The reference range for glycated nail protein (0.55-3.60mol/g nail) increases upon ageing. Results: In diabetics (n=112), values for glycated nail protein are significantly higher (median: 4.07mol/g nail, IQR: 2.37-6.89mol/g nail, P<0.0001) than in non-diabetics (n=116). ROC analysis shows an AUC of 0.848 (specificity 93.1%; sensitivity 68.9%). Conclusion: This affordable method is a simple alternative for diagnosing diabetes in remote areas as the pre-analytical phase (including all processes from the time a laboratory request is made by a physician until the sample is ready for testing) is extremely robust.
Keywords
GLYCOSYLATION, HAIR, HEMOGLOBIN, INDEX, HBA1C, BLOOD, HBA(1C), GLUCOSE, FRUCTOSAMINE, SUB-SAHARAN AFRICA, nails, keratin, hair, glycation, fructosamine

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Chicago
Sadiki Kishabongo, Antoine, Philippe Katchunga, Elisabeth Van Aken, Marijn Speeckaert, Sabrina Lagniau, DASHTY HUSEIN, Youri Taes, and Joris Delanghe. 2014. “Glycated Nail Proteins : a New Approach for Detecting Diabetes in Developing Countries.” Tropical Medicine & International Health 19 (1): 58–64.
APA
Sadiki Kishabongo, A., Katchunga, P., Van Aken, E., Speeckaert, M., Lagniau, S., HUSEIN, D., Taes, Y., et al. (2014). Glycated nail proteins : a new approach for detecting diabetes in developing countries. TROPICAL MEDICINE & INTERNATIONAL HEALTH, 19(1), 58–64.
Vancouver
1.
Sadiki Kishabongo A, Katchunga P, Van Aken E, Speeckaert M, Lagniau S, HUSEIN D, et al. Glycated nail proteins : a new approach for detecting diabetes in developing countries. TROPICAL MEDICINE & INTERNATIONAL HEALTH. 2014;19(1):58–64.
MLA
Sadiki Kishabongo, Antoine, Philippe Katchunga, Elisabeth Van Aken, et al. “Glycated Nail Proteins : a New Approach for Detecting Diabetes in Developing Countries.” TROPICAL MEDICINE & INTERNATIONAL HEALTH 19.1 (2014): 58–64. Print.
@article{4422418,
  abstract     = {Objective: To assess glycation of nail proteins as a tool in the diagnosis of diabetes.
Methods: Glycation of nail proteins was assessed using a modified photometric nitroblue tetrazolium-based assay, which provides information about average glucose values of the last 6-9months. Analysis is possible on 10mg of nail clippings with a within-run coefficient of variation (CV) of 11\%. The analyte is extremely stable. The reference range for glycated nail protein (0.55-3.60mol/g nail) increases upon ageing.
Results: In diabetics (n=112), values for glycated nail protein are significantly higher (median: 4.07mol/g nail, IQR: 2.37-6.89mol/g nail, P{\textlangle}0.0001) than in non-diabetics (n=116). ROC analysis shows an AUC of 0.848 (specificity 93.1\%; sensitivity 68.9\%).
Conclusion: This affordable method is a simple alternative for diagnosing diabetes in remote areas as the pre-analytical phase (including all processes from the time a laboratory request is made by a physician until the sample is ready for testing) is extremely robust.},
  author       = {Sadiki Kishabongo, Antoine and Katchunga, Philippe and Van Aken, Elisabeth and Speeckaert, Marijn and Lagniau, Sabrina and HUSEIN, DASHTY and Taes, Youri and Delanghe, Joris},
  issn         = {1360-2276},
  journal      = {TROPICAL MEDICINE \& INTERNATIONAL HEALTH},
  keyword      = {GLYCOSYLATION,HAIR,HEMOGLOBIN,INDEX,HBA1C,BLOOD,HBA(1C),GLUCOSE,FRUCTOSAMINE,SUB-SAHARAN AFRICA,nails,keratin,hair,glycation,fructosamine},
  language     = {eng},
  number       = {1},
  pages        = {58--64},
  title        = {Glycated nail proteins : a new approach for detecting diabetes in developing countries},
  url          = {http://dx.doi.org/10.1111/tmi.12218},
  volume       = {19},
  year         = {2014},
}

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