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Agglutination of intravenously administered phosphatidylcholine-containing lipid emulsions with serum C-reactive protein

Tugirimana Pierrot Lundimu, Marijn Speeckaert UGent, Tom Fiers, Marc De Buyzere, Jozef Kint, Dominique Benoit UGent and Joris Delanghe UGent (2013) NUTRITION IN CLINICAL PRACTICE. 28(2). p.253-259
abstract
Background: C-reactive protein (CRP) is able to bind phospholipids in the presence of calcium. We wanted to investigate the reaction of CRP with various commercial fat emulsions and to explore the impact of CRP agglutination on serum CRP levels. Materials and Methods: Serum specimens were mixed with Intralipid 20% (soybean oil-based fat emulsion), Structolipid (structured oil-based fat emulsion), Omegaven (fish oil-based fat emulsion), or SMOFlipid (mixed soybean oil-, olive oil-, and fish oil-based emulsion) in Tris-calcium buffer (pH 7.5). After 30 minutes of incubation at 37 degrees C, CRP-phospholipid complexes were turbidimetrically quantified and flow cytometric analysis was performed. Similarly, CRP complexes were monitored in vivo, following administration of fat emulsion. Results: CRP was able to agglutinate phospholipid-containing lipid droplets present in the soybean oil-based fat emulsion and the structured oil-based fat emulsion. To a lesser extent, agglutination was observed for fish oil-containing fat emulsions, whereas no agglutination was noticed for the mixed soybean oil-, olive oil-, and fish oil-based emulsion. Results for propofol-containing emulsions were comparable. Agglutination correlated with phospholipid content of the emulsions. When in vivo agglutination occurred, plasma CRP values dropped due to consumption of CRP by phospholipid-induced agglutination. Conclusions: In this in vitro experiment, we demonstrated agglutination of CRP with phospholipids in various fat emulsions. Research studies are required in patients to determine which effects occur with various intravenous fat emulsions.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
phospholipids, intravenous fat emulsions, PARENTERAL-NUTRITION, fish oils, inflammation, C-reactive protein, FAT-EMBOLISM, PHOSPHOLIPIDS, LIPOPROTEINS, OILS
journal title
NUTRITION IN CLINICAL PRACTICE
Nutr. Clin. Pract.
volume
28
issue
2
pages
253 - 259
Web of Science type
Article
Web of Science id
000317989000016
JCR category
NUTRITION & DIETETICS
JCR impact factor
2.058 (2013)
JCR rank
49/79 (2013)
JCR quartile
3 (2013)
ISSN
0884-5336
DOI
10.1177/0884533612474040
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4422281
handle
http://hdl.handle.net/1854/LU-4422281
date created
2014-06-18 10:11:19
date last changed
2016-12-19 15:38:52
@article{4422281,
  abstract     = {Background: C-reactive protein (CRP) is able to bind phospholipids in the presence of calcium. We wanted to investigate the reaction of CRP with various commercial fat emulsions and to explore the impact of CRP agglutination on serum CRP levels.
Materials and Methods: Serum specimens were mixed with Intralipid 20\% (soybean oil-based fat emulsion), Structolipid (structured oil-based fat emulsion), Omegaven (fish oil-based fat emulsion), or SMOFlipid (mixed soybean oil-, olive oil-, and fish oil-based emulsion) in Tris-calcium buffer (pH 7.5). After 30 minutes of incubation at 37 degrees C, CRP-phospholipid complexes were turbidimetrically quantified and flow cytometric analysis was performed. Similarly, CRP complexes were monitored in vivo, following administration of fat emulsion.
Results: CRP was able to agglutinate phospholipid-containing lipid droplets present in the soybean oil-based fat emulsion and the structured oil-based fat emulsion. To a lesser extent, agglutination was observed for fish oil-containing fat emulsions, whereas no agglutination was noticed for the mixed soybean oil-, olive oil-, and fish oil-based emulsion. Results for propofol-containing emulsions were comparable. Agglutination correlated with phospholipid content of the emulsions. When in vivo agglutination occurred, plasma CRP values dropped due to consumption of CRP by phospholipid-induced agglutination.
Conclusions: In this in vitro experiment, we demonstrated agglutination of CRP with phospholipids in various fat emulsions. Research studies are required in patients to determine which effects occur with various intravenous fat emulsions.},
  author       = {Lundimu, Tugirimana Pierrot and Speeckaert, Marijn and Fiers, Tom and De Buyzere, Marc and Kint, Jozef and Benoit, Dominique and Delanghe, Joris},
  issn         = {0884-5336},
  journal      = {NUTRITION IN CLINICAL PRACTICE},
  keyword      = {phospholipids,intravenous fat emulsions,PARENTERAL-NUTRITION,fish oils,inflammation,C-reactive protein,FAT-EMBOLISM,PHOSPHOLIPIDS,LIPOPROTEINS,OILS},
  language     = {eng},
  number       = {2},
  pages        = {253--259},
  title        = {Agglutination of intravenously administered phosphatidylcholine-containing lipid emulsions with serum C-reactive protein},
  url          = {http://dx.doi.org/10.1177/0884533612474040},
  volume       = {28},
  year         = {2013},
}

Chicago
Lundimu, Tugirimana Pierrot, Marijn Speeckaert, TOM FIERS, MARC DE BUYZERE, Jozef Kint, Dominique Benoit, and Joris Delanghe. 2013. “Agglutination of Intravenously Administered Phosphatidylcholine-containing Lipid Emulsions with Serum C-reactive Protein.” Nutrition in Clinical Practice 28 (2): 253–259.
APA
Lundimu, T. P., Speeckaert, M., FIERS, T., DE BUYZERE, M., Kint, J., Benoit, D., & Delanghe, J. (2013). Agglutination of intravenously administered phosphatidylcholine-containing lipid emulsions with serum C-reactive protein. NUTRITION IN CLINICAL PRACTICE, 28(2), 253–259.
Vancouver
1.
Lundimu TP, Speeckaert M, FIERS T, DE BUYZERE M, Kint J, Benoit D, et al. Agglutination of intravenously administered phosphatidylcholine-containing lipid emulsions with serum C-reactive protein. NUTRITION IN CLINICAL PRACTICE. 2013;28(2):253–9.
MLA
Lundimu, Tugirimana Pierrot, Marijn Speeckaert, TOM FIERS, et al. “Agglutination of Intravenously Administered Phosphatidylcholine-containing Lipid Emulsions with Serum C-reactive Protein.” NUTRITION IN CLINICAL PRACTICE 28.2 (2013): 253–259. Print.