Advanced search
1 file | 283.35 KB

Analysis of community composition of biofilms in a submerged filter system for the removal of ammonia and phenol from industrial wastewater

Author
Organization
Abstract
The bacterial diversity of a submerged filter, used for the removal of ammonia and phenol from an industrial wastewater with high salinity, was studied by a cultivation-independent approach based on PCR/TGGE (temperature-gradient gel electrophoresis). The wastewater treatment plant (laboratory scale) combined the nitrification and denitrification processes and consisted of two separated columns (one anoxic and one aerated) connected through a valve. The spatial diversity of bacterial communities in the plant biofilms was analysed by taking samples at four different heights in the system. TGGE profiles of PCR-amplified sequences of the 16 S rRNA gene (V3-hypervariable region) showed significant variations of the bacterial diversity, mainly depending on the concentration Of 02 along the system. Several bands separated by TGGE were reamplified and sequenced, in order to explore the composition of the microbial communities in the biofilms. Most of the sequenced bands (10 out of 13) were closely related to the 16 S rRNA gene of marine a proteol bacteria, mainly grouping in the periphery of the genus Roseobacter. other sequences were related to those of gamma-proteobacteria, the nitrite oxidizer Nitrospira marina and anaerobic phencil-degrading bacteria of the Desulfobacteraceae.
Keywords
PCR, biofilm, POPULATIONS, BACTERIA, SEAWATER, GENES, 16S RIBOSOMAL-RNA, gel electrophoresis (TGGE), temperature-gradient, phenol biodegradation, biofilter, denitrification, biodiversity

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 283.35 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Cortés-Lorenzo, C, ML Molina-Muñoz, B Gómez-Villalba, Ramiro Vilchez Vargas, A Ramos, B Rodelas, E Hontoria, and J González-López. 2006. “Analysis of Community Composition of Biofilms in a Submerged Filter System for the Removal of Ammonia and Phenol from Industrial Wastewater.” Biochemical Society Transactions 34 (1): 165–168.
APA
Cortés-Lorenzo, C., Molina-Muñoz, M., Gómez-Villalba, B., Vilchez Vargas, R., Ramos, A., Rodelas, B., Hontoria, E., et al. (2006). Analysis of community composition of biofilms in a submerged filter system for the removal of ammonia and phenol from industrial wastewater. BIOCHEMICAL SOCIETY TRANSACTIONS, 34(1), 165–168. Presented at the 11th Nitrogen Cycle Meeting.
Vancouver
1.
Cortés-Lorenzo C, Molina-Muñoz M, Gómez-Villalba B, Vilchez Vargas R, Ramos A, Rodelas B, et al. Analysis of community composition of biofilms in a submerged filter system for the removal of ammonia and phenol from industrial wastewater. BIOCHEMICAL SOCIETY TRANSACTIONS. 2006;34(1):165–8.
MLA
Cortés-Lorenzo, C, ML Molina-Muñoz, B Gómez-Villalba, et al. “Analysis of Community Composition of Biofilms in a Submerged Filter System for the Removal of Ammonia and Phenol from Industrial Wastewater.” BIOCHEMICAL SOCIETY TRANSACTIONS 34.1 (2006): 165–168. Print.
@article{4421226,
  abstract     = {The bacterial diversity of a submerged filter, used for the removal of ammonia and phenol from an industrial wastewater with high salinity, was studied by a cultivation-independent approach based on PCR/TGGE (temperature-gradient gel electrophoresis). The wastewater treatment plant (laboratory scale) combined the nitrification and denitrification processes and consisted of two separated columns (one anoxic and one aerated) connected through a valve. The spatial diversity of bacterial communities in the plant biofilms was analysed by taking samples at four different heights in the system. TGGE profiles of PCR-amplified sequences of the 16 S rRNA gene (V3-hypervariable region) showed significant variations of the bacterial diversity, mainly depending on the concentration Of 02 along the system. Several bands separated by TGGE were reamplified and sequenced, in order to explore the composition of the microbial communities in the biofilms. Most of the sequenced bands (10 out of 13) were closely related to the 16 S rRNA gene of marine a proteol bacteria, mainly grouping in the periphery of the genus Roseobacter. other sequences were related to those of gamma-proteobacteria, the nitrite oxidizer Nitrospira marina and anaerobic phencil-degrading bacteria of the Desulfobacteraceae.},
  author       = {Cortés-Lorenzo, C and Molina-Muñoz, ML and Gómez-Villalba, B and Vilchez Vargas, Ramiro and Ramos, A and Rodelas, B and Hontoria, E and González-López, J},
  issn         = {0300-5127},
  journal      = {BIOCHEMICAL SOCIETY TRANSACTIONS},
  keywords     = {PCR,biofilm,POPULATIONS,BACTERIA,SEAWATER,GENES,16S RIBOSOMAL-RNA,gel electrophoresis (TGGE),temperature-gradient,phenol biodegradation,biofilter,denitrification,biodiversity},
  language     = {eng},
  location     = {Granada, Spain},
  number       = {1},
  pages        = {165--168},
  title        = {Analysis of community composition of biofilms in a submerged filter system for the removal of ammonia and phenol from industrial wastewater},
  url          = {http://dx.doi.org/10.1042/BST0340165},
  volume       = {34},
  year         = {2006},
}

Altmetric
View in Altmetric
Web of Science
Times cited: