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Combining growth-promoting genes leads to positive epistasis in Arabidopsis thaliana

(2014) eLIFE. 3.
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Biotechnology for a sustainable economy (Bio-Economy)
Abstract
Several genes have been described to positively influence final leaf size in Arabidopsis when mutated or overexpressed. However, the connections between these growth regulating genes are still poorly understood. Clearly such knowledge would significantly contribute to understand the biological processes driving leaf growth. In this study, we performed a combinatorial screen with thirteen transgenic Arabidopsis lines with an increased leaf size. Surprisingly, we found that from 61 analyzed combinations, 39% showed an additional increase in leaf size and most of these resulted from a positive epistasis on growth. Similar to what is found in other organisms in which such an epistasis assay was performed, only few genes were highly connected in synergistic combinations. We also observed a positive epistasis in the majority of the combinations with samba, BR11(OE) or SAUR19(OE), suggesting that these growth regulators are more prone to lead to synergistic effects in binary combinations. Furthermore, positive epistasis was not only found with combinations of genes with a similar mode of action, but also with genes which affect distinct processes, such as cell proliferation and cell expansion.
Keywords
TRANSCRIPTION FACTOR, FINAL ORGAN SIZE, QUANTITATIVE TRAITS, AUXIN, CELL, FITNESS, YEAST, PROLIFERATION, EXPRESSION, EXPANSION

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Citation

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MLA
Vanhaeren, Hannes et al. “Combining Growth-promoting Genes Leads to Positive Epistasis in Arabidopsis Thaliana.” eLIFE 3 (2014): n. pag. Print.
APA
Vanhaeren, H., Gonzalez Sanchez, N., Coppens, F., De Milde, L., Van Daele, T., Vermeersch, M., Eloy, N. B., et al. (2014). Combining growth-promoting genes leads to positive epistasis in Arabidopsis thaliana. eLIFE, 3.
Chicago author-date
Vanhaeren, Hannes, Nathalie Gonzalez Sanchez, Frederik Coppens, Liesbeth De Milde, Twiggy Van Daele, Mattias Vermeersch, Nubia Barbosa Eloy, Veronique Storme, and Dirk Inzé. 2014. “Combining Growth-promoting Genes Leads to Positive Epistasis in Arabidopsis Thaliana.” eLIFE 3.
Chicago author-date (all authors)
Vanhaeren, Hannes, Nathalie Gonzalez Sanchez, Frederik Coppens, Liesbeth De Milde, Twiggy Van Daele, Mattias Vermeersch, Nubia Barbosa Eloy, Veronique Storme, and Dirk Inzé. 2014. “Combining Growth-promoting Genes Leads to Positive Epistasis in Arabidopsis Thaliana.” eLIFE 3.
Vancouver
1.
Vanhaeren H, Gonzalez Sanchez N, Coppens F, De Milde L, Van Daele T, Vermeersch M, et al. Combining growth-promoting genes leads to positive epistasis in Arabidopsis thaliana. eLIFE. 2014;3.
IEEE
[1]
H. Vanhaeren et al., “Combining growth-promoting genes leads to positive epistasis in Arabidopsis thaliana,” eLIFE, vol. 3, 2014.
@article{4420343,
  abstract     = {Several genes have been described to positively influence final leaf size in Arabidopsis when mutated or overexpressed. However, the connections between these growth regulating genes are still poorly understood. Clearly such knowledge would significantly contribute to understand the biological processes driving leaf growth. In this study, we performed a combinatorial screen with thirteen transgenic Arabidopsis lines with an increased leaf size. Surprisingly, we found that from 61 analyzed combinations, 39% showed an additional increase in leaf size and most of these resulted from a positive epistasis on growth. Similar to what is found in other organisms in which such an epistasis assay was performed, only few genes were highly connected in synergistic combinations. We also observed a positive epistasis in the majority of the combinations with samba, BR11(OE) or SAUR19(OE), suggesting that these growth regulators are more prone to lead to synergistic effects in binary combinations. Furthermore, positive epistasis was not only found with combinations of genes with a similar mode of action, but also with genes which affect distinct processes, such as cell proliferation and cell expansion.},
  articleno    = {e02252},
  author       = {Vanhaeren, Hannes and Gonzalez Sanchez, Nathalie and Coppens, Frederik and De Milde, Liesbeth and Van Daele, Twiggy and Vermeersch, Mattias and Eloy, Nubia Barbosa and Storme, Veronique and Inzé, Dirk},
  issn         = {2050-084X},
  journal      = {eLIFE},
  keywords     = {TRANSCRIPTION FACTOR,FINAL ORGAN SIZE,QUANTITATIVE TRAITS,AUXIN,CELL,FITNESS,YEAST,PROLIFERATION,EXPRESSION,EXPANSION},
  language     = {eng},
  pages        = {19},
  title        = {Combining growth-promoting genes leads to positive epistasis in Arabidopsis thaliana},
  url          = {http://dx.doi.org/10.7554/eLife.02252},
  volume       = {3},
  year         = {2014},
}

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