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Plant-derived decapeptide OSIP108 interferes with Candida albicans biofilm formation without affecting cell viability

Nicolas Delattin, Katrijn De Brucker, David J Craik, Olivier Cheneval, Mirjam Fröhlich, Matija Veber, Lenart Girandon, Talya R Davis, Anne E Weeks, Carol A Kumamoto, et al. (2014) ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 58(5). p.2647-2656
abstract
We previously identified a decapeptide from the model plant Arabidopsis thaliana, OSIP108, which is induced upon fungal pathogen infection. In this study, we demonstrated that OSIP108 interferes with biofilm formation of the fungal pathogen Candida albicans without affecting the viability or growth of C. albicans cells. OSIP108 displayed no cytotoxicity against various human cell lines. Furthermore, OSIP108 enhanced the activity of the antifungal agents amphotericin B and caspofungin in vitro and in vivo in a Caenorhabditis elegans-C. albicans biofilm infection model. These data point to the potential use of OSIP108 in combination therapy with conventional antifungal agents. In a first attempt to unravel its mode of action, we screened a library of 137 homozygous C. albicans mutants, affected in genes encoding cell wall proteins or transcription factors important for biofilm formation, for altered OSIP108 sensitivity. We identified 9 OSIP108-tolerant C. albicans mutants that were defective in either components important for cell wall integrity or the yeast-to-hypha transition. In line with these findings, we demonstrated that OSIP108 activates the C. albicans cell wall integrity pathway and that its antibiofilm activity can be blocked by compounds inhibiting the yeast-to-hypha transition. Furthermore, we found that OSIP108 is predominantly localized at the C. albicans cell surface. These data point to interference of OSIP108 with cell wall-related processes of C. albicans, resulting in impaired biofilm formation.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SCANNING MUTAGENESIS, FUNGAL-INFECTIONS, ANTIFUNGAL SUSCEPTIBILITY, CAENORHABDITIS-ELEGANS, HOST-PATHOGEN INTERACTIONS, GENE-EXPRESSION, WALL INTEGRITY, VIRULENCE, PROTEINS, STRESS
journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Antimicrob. Agents Chemother.
volume
58
issue
5
pages
2647 - 2656
Web of Science type
Article
Web of Science id
000334364300019
JCR category
PHARMACOLOGY & PHARMACY
JCR impact factor
4.476 (2014)
JCR rank
27/255 (2014)
JCR quartile
1 (2014)
ISSN
0066-4804
DOI
10.1128/AAC.01274-13
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4419934
handle
http://hdl.handle.net/1854/LU-4419934
date created
2014-06-17 08:55:02
date last changed
2016-12-19 15:39:39
@article{4419934,
  abstract     = {We previously identified a decapeptide from the model plant Arabidopsis thaliana, OSIP108, which is induced upon fungal pathogen infection. In this study, we demonstrated that OSIP108 interferes with biofilm formation of the fungal pathogen Candida albicans without affecting the viability or growth of C. albicans cells. OSIP108 displayed no cytotoxicity against various human cell lines. Furthermore, OSIP108 enhanced the activity of the antifungal agents amphotericin B and caspofungin in vitro and in vivo in a Caenorhabditis elegans-C. albicans biofilm infection model. These data point to the potential use of OSIP108 in combination therapy with conventional antifungal agents. In a first attempt to unravel its mode of action, we screened a library of 137 homozygous C. albicans mutants, affected in genes encoding cell wall proteins or transcription factors important for biofilm formation, for altered OSIP108 sensitivity. We identified 9 OSIP108-tolerant C. albicans mutants that were defective in either components important for cell wall integrity or the yeast-to-hypha transition. In line with these findings, we demonstrated that OSIP108 activates the C. albicans cell wall integrity pathway and that its antibiofilm activity can be blocked by compounds inhibiting the yeast-to-hypha transition. Furthermore, we found that OSIP108 is predominantly localized at the C. albicans cell surface. These data point to interference of OSIP108 with cell wall-related processes of C. albicans, resulting in impaired biofilm formation.},
  author       = {Delattin, Nicolas and De Brucker, Katrijn and Craik, David J and Cheneval, Olivier and Fr{\"o}hlich, Mirjam and Veber, Matija and Girandon, Lenart and Davis, Talya R and Weeks, Anne E and Kumamoto, Carol A and Cos, Paul and Coenye, Tom and De Coninck, Barbara and Cammue, Bruno PA and Thevissen, Karin},
  issn         = {0066-4804},
  journal      = {ANTIMICROBIAL AGENTS AND CHEMOTHERAPY},
  keyword      = {SCANNING MUTAGENESIS,FUNGAL-INFECTIONS,ANTIFUNGAL SUSCEPTIBILITY,CAENORHABDITIS-ELEGANS,HOST-PATHOGEN INTERACTIONS,GENE-EXPRESSION,WALL INTEGRITY,VIRULENCE,PROTEINS,STRESS},
  language     = {eng},
  number       = {5},
  pages        = {2647--2656},
  title        = {Plant-derived decapeptide OSIP108 interferes with Candida albicans biofilm formation without affecting cell viability},
  url          = {http://dx.doi.org/10.1128/AAC.01274-13},
  volume       = {58},
  year         = {2014},
}

Chicago
Delattin, Nicolas, Katrijn De Brucker, David J Craik, Olivier Cheneval, Mirjam Fröhlich, Matija Veber, Lenart Girandon, et al. 2014. “Plant-derived Decapeptide OSIP108 Interferes with Candida Albicans Biofilm Formation Without Affecting Cell Viability.” Antimicrobial Agents and Chemotherapy 58 (5): 2647–2656.
APA
Delattin, N., De Brucker, K., Craik, D. J., Cheneval, O., Fröhlich, M., Veber, M., Girandon, L., et al. (2014). Plant-derived decapeptide OSIP108 interferes with Candida albicans biofilm formation without affecting cell viability. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 58(5), 2647–2656.
Vancouver
1.
Delattin N, De Brucker K, Craik DJ, Cheneval O, Fröhlich M, Veber M, et al. Plant-derived decapeptide OSIP108 interferes with Candida albicans biofilm formation without affecting cell viability. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY. 2014;58(5):2647–56.
MLA
Delattin, Nicolas, Katrijn De Brucker, David J Craik, et al. “Plant-derived Decapeptide OSIP108 Interferes with Candida Albicans Biofilm Formation Without Affecting Cell Viability.” ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 58.5 (2014): 2647–2656. Print.