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Library construction for next-generation sequencing: overviews and challenges

(2014) BIOTECHNIQUES. 56(2). p.61-77
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Abstract
High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing exponentially. Here, we examine the critical role of sequencing library quality and consider important challenges when preparing NGS libraries from DNA and RNA sources. Factors such as the quantity and physical characteristics of the RNA or DNA source material as well as the desired application (i.e., genome sequencing, targeted sequencing, RNA-seq, ChIP-seq, RIP-seq, and methylation) are addressed in the context of preparing high quality sequencing libraries. In addition, the current methods for preparing NGS libraries from single cells are also discussed.
Keywords
CHIP-SEQ, IN-VIVO, GENE-EXPRESSION, WIDE IDENTIFICATION, ANALYSIS REVEALS, EMBRYONIC STEM-CELLS, SINGLE-BASE RESOLUTION, RNA-BINDING PROTEINS, DNA METHYLATION ANALYSIS, ChIP-seq, RIP-seq, RNA-seq, DNA-seq, deep sequencing, DNA, RNA, library preparation, next-generation sequencing, NONCODING RNAS

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Citation

Please use this url to cite or link to this publication:

MLA
Head, Steven R, H Kiyomi Komori, Sarah A LaMere, et al. “Library Construction for Next-generation Sequencing: Overviews and Challenges.” BIOTECHNIQUES 56.2 (2014): 61–77. Print.
APA
Head, S. R., Komori, H. K., LaMere, S. A., Whisenant, T., Van Nieuwerburgh, F., Salomon, D. R., & Ordoukhanian, P. (2014). Library construction for next-generation sequencing: overviews and challenges. BIOTECHNIQUES, 56(2), 61–77.
Chicago author-date
Head, Steven R, H Kiyomi Komori, Sarah A LaMere, Thomas Whisenant, Filip Van Nieuwerburgh, Daniel R Salomon, and Phillip Ordoukhanian. 2014. “Library Construction for Next-generation Sequencing: Overviews and Challenges.” Biotechniques 56 (2): 61–77.
Chicago author-date (all authors)
Head, Steven R, H Kiyomi Komori, Sarah A LaMere, Thomas Whisenant, Filip Van Nieuwerburgh, Daniel R Salomon, and Phillip Ordoukhanian. 2014. “Library Construction for Next-generation Sequencing: Overviews and Challenges.” Biotechniques 56 (2): 61–77.
Vancouver
1.
Head SR, Komori HK, LaMere SA, Whisenant T, Van Nieuwerburgh F, Salomon DR, et al. Library construction for next-generation sequencing: overviews and challenges. BIOTECHNIQUES. 2014;56(2):61–77.
IEEE
[1]
S. R. Head et al., “Library construction for next-generation sequencing: overviews and challenges,” BIOTECHNIQUES, vol. 56, no. 2, pp. 61–77, 2014.
@article{4416337,
  abstract     = {High-throughput sequencing, also known as next-generation sequencing (NGS), has revolutionized genomic research. In recent years, NGS technology has steadily improved, with costs dropping and the number and range of sequencing applications increasing exponentially. Here, we examine the critical role of sequencing library quality and consider important challenges when preparing NGS libraries from DNA and RNA sources. Factors such as the quantity and physical characteristics of the RNA or DNA source material as well as the desired application (i.e., genome sequencing, targeted sequencing, RNA-seq, ChIP-seq, RIP-seq, and methylation) are addressed in the context of preparing high quality sequencing libraries. In addition, the current methods for preparing NGS libraries from single cells are also discussed.},
  author       = {Head, Steven R and Komori, H Kiyomi and LaMere, Sarah A and Whisenant, Thomas and Van Nieuwerburgh, Filip and Salomon, Daniel R and Ordoukhanian, Phillip},
  issn         = {0736-6205},
  journal      = {BIOTECHNIQUES},
  keywords     = {CHIP-SEQ,IN-VIVO,GENE-EXPRESSION,WIDE IDENTIFICATION,ANALYSIS REVEALS,EMBRYONIC STEM-CELLS,SINGLE-BASE RESOLUTION,RNA-BINDING PROTEINS,DNA METHYLATION ANALYSIS,ChIP-seq,RIP-seq,RNA-seq,DNA-seq,deep sequencing,DNA,RNA,library preparation,next-generation sequencing,NONCODING RNAS},
  language     = {eng},
  number       = {2},
  pages        = {61--77},
  title        = {Library construction for next-generation sequencing: overviews and challenges},
  url          = {http://dx.doi.org/10.2144/000114133},
  volume       = {56},
  year         = {2014},
}

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