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Pathway of nitrous oxide consumption in isolated Pseudomonas stutzeri strains under anoxic and oxic conditions

Joachim Desloover (UGent) , Dries Roobroeck (UGent) , Kim Heylen (UGent) , Sebastià Puig, Pascal Boeckx (UGent) , Willy Verstraete (UGent) and Nico Boon (UGent)
(2014) ENVIRONMENTAL MICROBIOLOGY. 16(10). p.3143-3152
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Abstract
The microbial consumption of nitrous oxide (N2O) has gained great interest since it was revealed that this process could mitigate the greenhouse effect of N2O. The consumption of N2O results from its reduction to dinitrogen gas (N-2) as part of the denitrification process. However, there is ongoing debate regarding an alternative pathway, namely reduction of N2O to NH4+, or assimilatory N2O consumption. To date, this pathway is poorly investigated and lacks unambiguous evidence. Enrichment of denitrifying activated sludge using a mineral nitrogen-free medium rendered a mixed culture capable of anoxic and oxic N2O consumption. Dilution plating, isolation and deoxyribonucleic acid fingerprinting identified a collection of Pseudomonas stutzeri strains as dominant N2O consumers in both anaerobic and aerobic enrichments. A detailed isotope tracing experiment with a Pseudomonas stutzeri isolate showed that consumption of N2O via assimilatory reduction to NH4+ was absent. Conversely, respiratory N2O reduction was directly coupled to N-2 fixation.
Keywords
COMMUNITIES, FIXATION, DENITRIFICATION, N2O, TREATMENT PLANTS, ALCALIGENES-FAECALIS, DENITRIFYING BACTERIA, WATER TREATMENT SYSTEM, 16S RIBOSOMAL-RNA, GRADIENT GEL-ELECTROPHORESIS

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Chicago
Desloover, Joachim, Dries Roobroeck, Kim Heylen, Sebastià Puig, Pascal Boeckx, Willy Verstraete, and Nico Boon. 2014. “Pathway of Nitrous Oxide Consumption in Isolated Pseudomonas Stutzeri Strains Under Anoxic and Oxic Conditions.” Environmental Microbiology 16 (10): 3143–3152.
APA
Desloover, J., Roobroeck, D., Heylen, K., Puig, S., Boeckx, P., Verstraete, W., & Boon, N. (2014). Pathway of nitrous oxide consumption in isolated Pseudomonas stutzeri strains under anoxic and oxic conditions. ENVIRONMENTAL MICROBIOLOGY, 16(10), 3143–3152.
Vancouver
1.
Desloover J, Roobroeck D, Heylen K, Puig S, Boeckx P, Verstraete W, et al. Pathway of nitrous oxide consumption in isolated Pseudomonas stutzeri strains under anoxic and oxic conditions. ENVIRONMENTAL MICROBIOLOGY. 2014;16(10):3143–52.
MLA
Desloover, Joachim, Dries Roobroeck, Kim Heylen, et al. “Pathway of Nitrous Oxide Consumption in Isolated Pseudomonas Stutzeri Strains Under Anoxic and Oxic Conditions.” ENVIRONMENTAL MICROBIOLOGY 16.10 (2014): 3143–3152. Print.
@article{4413609,
  abstract     = {The microbial consumption of nitrous oxide (N2O) has gained great interest since it was revealed that this process could mitigate the greenhouse effect of N2O. The consumption of N2O results from its reduction to dinitrogen gas (N-2) as part of the denitrification process. However, there is ongoing debate regarding an alternative pathway, namely reduction of N2O to NH4+, or assimilatory N2O consumption. To date, this pathway is poorly investigated and lacks unambiguous evidence. Enrichment of denitrifying activated sludge using a mineral nitrogen-free medium rendered a mixed culture capable of anoxic and oxic N2O consumption. Dilution plating, isolation and deoxyribonucleic acid fingerprinting identified a collection of Pseudomonas stutzeri strains as dominant N2O consumers in both anaerobic and aerobic enrichments. A detailed isotope tracing experiment with a Pseudomonas stutzeri isolate showed that consumption of N2O via assimilatory reduction to NH4+ was absent. Conversely, respiratory N2O reduction was directly coupled to N-2 fixation.},
  author       = {Desloover, Joachim and Roobroeck, Dries and Heylen, Kim and Puig, Sebasti{\`a} and Boeckx, Pascal and Verstraete, Willy and Boon, Nico},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keyword      = {COMMUNITIES,FIXATION,DENITRIFICATION,N2O,TREATMENT PLANTS,ALCALIGENES-FAECALIS,DENITRIFYING BACTERIA,WATER TREATMENT SYSTEM,16S RIBOSOMAL-RNA,GRADIENT GEL-ELECTROPHORESIS},
  language     = {eng},
  number       = {10},
  pages        = {3143--3152},
  title        = {Pathway of nitrous oxide consumption in isolated Pseudomonas stutzeri strains under anoxic and oxic conditions},
  url          = {http://dx.doi.org/10.1111/1462-2920.12404},
  volume       = {16},
  year         = {2014},
}

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