Folate profiling in potato (Solanum tuberosum) tubers by ultrahigh-performance liquid chromatography-tandem mass spectrometry
- Author
- Jeroen Van Daele (UGent) , Dieter Blancquaert (UGent) , Filip Kiekens (UGent) , Dominique Van Der Straeten (UGent) , Willy Lambert (UGent) and Christophe Stove (UGent)
- Organization
- Abstract
- An ultrahigh-performance liquid chromatography tandem mass spectrometry method was developed and validated for the profiling of six folate species in potatoes. The calibration curves cover a wide, linear range (the lower and upper limits of quantitation range between 0.22-0.24 and 216.07-242.28 mu g/100 g of fresh weight), allowing sensitive determination in small amounts of potato flesh. With a single exception, the acceptance criteria for intra- and interday precision and accuracy were met: for all quality controls, the percent relative standard deviation and the percent bias were lower than 15% (or 20% at the lower limit of quantitation). Application of the method on tubers at different stages of maturation demonstrated the large variability within a single variety: the folate content and polyglutamylation rate varied between 10.35 and 24.01 mu g/100 g of fresh weight and between 4.96% and 60.49%, respectively. Additionally, the two-dimensional folate profiling of mature tubers demonstrated an increase in folate from center to peel, combined with a stable species distribution and polyglutamylation rate.
- Keywords
- folate profiling, folate, potato, Solanum tuberosum, UHPLC-MS/MS, tandem mass spectrometry, variability, FOLIC-ACID, MICROBIOLOGICAL ASSAY, BIOANALYTICAL METHODS, TRIENZYME EXTRACTION, FOODS, VEGETABLES, RETENTION, ENHANCEMENT, OPTIMIZATION, STABILITY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-4412309
- MLA
- Van Daele, Jeroen, et al. “Folate Profiling in Potato (Solanum Tuberosum) Tubers by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 62, no. 14, 2014, pp. 3092–100, doi:10.1021/jf500753v.
- APA
- Van Daele, J., Blancquaert, D., Kiekens, F., Van Der Straeten, D., Lambert, W., & Stove, C. (2014). Folate profiling in potato (Solanum tuberosum) tubers by ultrahigh-performance liquid chromatography-tandem mass spectrometry. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 62(14), 3092–3100. https://doi.org/10.1021/jf500753v
- Chicago author-date
- Van Daele, Jeroen, Dieter Blancquaert, Filip Kiekens, Dominique Van Der Straeten, Willy Lambert, and Christophe Stove. 2014. “Folate Profiling in Potato (Solanum Tuberosum) Tubers by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62 (14): 3092–3100. https://doi.org/10.1021/jf500753v.
- Chicago author-date (all authors)
- Van Daele, Jeroen, Dieter Blancquaert, Filip Kiekens, Dominique Van Der Straeten, Willy Lambert, and Christophe Stove. 2014. “Folate Profiling in Potato (Solanum Tuberosum) Tubers by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62 (14): 3092–3100. doi:10.1021/jf500753v.
- Vancouver
- 1.Van Daele J, Blancquaert D, Kiekens F, Van Der Straeten D, Lambert W, Stove C. Folate profiling in potato (Solanum tuberosum) tubers by ultrahigh-performance liquid chromatography-tandem mass spectrometry. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2014;62(14):3092–100.
- IEEE
- [1]J. Van Daele, D. Blancquaert, F. Kiekens, D. Van Der Straeten, W. Lambert, and C. Stove, “Folate profiling in potato (Solanum tuberosum) tubers by ultrahigh-performance liquid chromatography-tandem mass spectrometry,” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 62, no. 14, pp. 3092–3100, 2014.
@article{4412309,
abstract = {{An ultrahigh-performance liquid chromatography tandem mass spectrometry method was developed and validated for the profiling of six folate species in potatoes. The calibration curves cover a wide, linear range (the lower and upper limits of quantitation range between 0.22-0.24 and 216.07-242.28 mu g/100 g of fresh weight), allowing sensitive determination in small amounts of potato flesh. With a single exception, the acceptance criteria for intra- and interday precision and accuracy were met: for all quality controls, the percent relative standard deviation and the percent bias were lower than 15% (or 20% at the lower limit of quantitation). Application of the method on tubers at different stages of maturation demonstrated the large variability within a single variety: the folate content and polyglutamylation rate varied between 10.35 and 24.01 mu g/100 g of fresh weight and between 4.96% and 60.49%, respectively. Additionally, the two-dimensional folate profiling of mature tubers demonstrated an increase in folate from center to peel, combined with a stable species distribution and polyglutamylation rate.}},
author = {{Van Daele, Jeroen and Blancquaert, Dieter and Kiekens, Filip and Van Der Straeten, Dominique and Lambert, Willy and Stove, Christophe}},
issn = {{0021-8561}},
journal = {{JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY}},
keywords = {{folate profiling,folate,potato,Solanum tuberosum,UHPLC-MS/MS,tandem mass spectrometry,variability,FOLIC-ACID,MICROBIOLOGICAL ASSAY,BIOANALYTICAL METHODS,TRIENZYME EXTRACTION,FOODS,VEGETABLES,RETENTION,ENHANCEMENT,OPTIMIZATION,STABILITY}},
language = {{eng}},
number = {{14}},
pages = {{3092--3100}},
title = {{Folate profiling in potato (Solanum tuberosum) tubers by ultrahigh-performance liquid chromatography-tandem mass spectrometry}},
url = {{http://dx.doi.org/10.1021/jf500753v}},
volume = {{62}},
year = {{2014}},
}
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