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Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition

Bruno Vlaeminck UGent, Tamara Braeckman and Veerle Fievez UGent (2014) LIPIDS. 49(6). p.517-525
abstract
Ruminal disappearance of linoleic and linolenic acid has been studied extensively. Less is known of the metabolism of docosahexaenoic acid (22:6n-3). The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep. In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated. A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL). In experiment 3, several concentrations of 22:6n-3 (0.05–0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose). In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated. In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium. Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance. A greater proportion of rumen fluid resulted in increased disappearance of 22:6n-3. The effect of carbohydrate type was small compared with the former two factors. These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Biohydrogenation, Metabolites, Rumen, Docosahexaenoic acid, UNSATURATED FATTY-ACIDS, DOCOSAHEXAENOIC ACID, LINOLEIC-ACID, MILK-FAT, FISH-OIL, BUTYRIVIBRIO-FIBRISOLVENS, RUMINAL BIOHYDROGENATION, CULTURES, INCUBATION, ISOMERS
journal title
LIPIDS
Lipids
volume
49
issue
6
pages
517 - 525
Web of Science type
Article
Web of Science id
000336805400002
JCR category
NUTRITION & DIETETICS
JCR impact factor
1.854 (2014)
JCR rank
53/77 (2014)
JCR quartile
3 (2014)
ISSN
0024-4201
DOI
10.1007/s11745-014-3905-8
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4411913
handle
http://hdl.handle.net/1854/LU-4411913
date created
2014-06-11 13:10:35
date last changed
2016-12-19 15:43:02
@article{4411913,
  abstract     = {Ruminal disappearance of linoleic and linolenic acid has been studied extensively. Less is known of the metabolism of docosahexaenoic acid (22:6n-3). The aim of this study was to identify factors which affect the disappearance of 22:6n-3 during in vitro batch incubations using rumen fluid from sheep. In experiment 1, the effect of the rumen fluid/buffer ratio (0.2 or 0.4), substrate (cellulose or cellulose/glucose), time of 22:6n-3 addition (0.08 mg/mL after 0 or 6 h of incubation) and incubation time (24 or 48 h) was evaluated. A mixture design was used in experiment 2 to evaluate the effect of carbohydrate type (cellulose, glucose, cellobiose and starch) on 22:6n-3 disappearance (0.08 mg/mL). In experiment 3, several concentrations of 22:6n-3 (0.05--0.30 mg/mL) were evaluated with different substrate mixtures (combinations of cellobiose, starch and cellulose). In a final experiment, the effect of the rumen fluid/buffer ratio (0.20, 0.35 and 0.50) and substrate (glucose, cellobiose and starch) was evaluated. In this experiment, 22:6n-3 was added as a proportion of rumen fluid ranging from 0.1 to 0.4 mg/mL rumen fluid, contrary to former experiments where concentrations were relative to culture medium. Low levels of 22:6n-3 (0.05 mg/mL) allowed extensive metabolism whereas increasing amounts of 22:6n-3 hampered its disappearance. A greater proportion of rumen fluid resulted in increased disappearance of 22:6n-3. The effect of carbohydrate type was small compared with the former two factors. These results suggest that in vitro metabolism of 22:6n-3 is mostly dictated by the conditions at the start of the incubation, i.e., inoculum, probably reflecting the density of bacteria able to metabolize 22:6n-3.},
  author       = {Vlaeminck, Bruno and Braeckman, Tamara and Fievez, Veerle},
  issn         = {0024-4201},
  journal      = {LIPIDS},
  keyword      = {Biohydrogenation,Metabolites,Rumen,Docosahexaenoic acid,UNSATURATED FATTY-ACIDS,DOCOSAHEXAENOIC ACID,LINOLEIC-ACID,MILK-FAT,FISH-OIL,BUTYRIVIBRIO-FIBRISOLVENS,RUMINAL BIOHYDROGENATION,CULTURES,INCUBATION,ISOMERS},
  language     = {eng},
  number       = {6},
  pages        = {517--525},
  title        = {Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition},
  url          = {http://dx.doi.org/10.1007/s11745-014-3905-8},
  volume       = {49},
  year         = {2014},
}

Chicago
Vlaeminck, Bruno, Tamara Braeckman, and Veerle Fievez. 2014. “Rumen Metabolism of 22:6n-3 in Vitro Is Dependent on Its Concentration and Inoculum Size, but Less Dependent on Substrate Carbohydrate Composition.” Lipids 49 (6): 517–525.
APA
Vlaeminck, B., Braeckman, T., & Fievez, V. (2014). Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition. LIPIDS, 49(6), 517–525.
Vancouver
1.
Vlaeminck B, Braeckman T, Fievez V. Rumen metabolism of 22:6n-3 in vitro is dependent on its concentration and inoculum size, but less dependent on substrate carbohydrate composition. LIPIDS. 2014;49(6):517–25.
MLA
Vlaeminck, Bruno, Tamara Braeckman, and Veerle Fievez. “Rumen Metabolism of 22:6n-3 in Vitro Is Dependent on Its Concentration and Inoculum Size, but Less Dependent on Substrate Carbohydrate Composition.” LIPIDS 49.6 (2014): 517–525. Print.