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Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC-MS

Thomas Van Damme UGent, Miroslava Lachova UGent, Frederic Lynen UGent, Roman Szucs UGent and Patrick Sandra UGent (2014) ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 406(2). p.401-407
abstract
Analysis of drugs and metabolites in biological matrices such as blood or plasma by LC-MS is routinely challenged by the presence of large quantities of competing molecules for ionization in soft ionization sources, such as proteins and phospholipids. While the former can easily be removed by protein precipitation, pre-analytical extraction of the latter is necessary because they show very high retention in reversed-phase LC resulting in long analysis times or in ion suppression effects when not eluted before the next runs. A novel HILIC-based SPE approach, making use of silica cartridges and of acetone as organic solvent, is introduced as a potent alternative to current commercial methods for phospholipid removal. The methodology was developed and tested for a broad polarity range of pharmaceutical solutes (log P from 0 to 6.6) and broad applicability can therefore be envisaged.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Acetone as eluting solvent, HILIC SPE, TANDEM MASS-SPECTROMETRY, Phospholipid removal, Ion suppression, Matrix effects, LC-MS, INTERNAL STANDARD, TRIFLUOROACETIC-ACID, ION-PAIRING REAGENTS, HUMAN PLASMA, ELECTROSPRAY-IONIZATION, ESI-MS, SIGNAL SUPPRESSION, SAMPLE PREPARATION, PROTEIN PRECIPITATION
journal title
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
Anal. Bioanal. Chem.
volume
406
issue
2
pages
401 - 407
Web of Science type
Article
Web of Science id
000329626600003
JCR category
CHEMISTRY, ANALYTICAL
JCR impact factor
3.436 (2014)
JCR rank
13/74 (2014)
JCR quartile
1 (2014)
ISSN
1618-2642
DOI
10.1007/s00216-013-7281-7
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
4411554
handle
http://hdl.handle.net/1854/LU-4411554
date created
2014-06-10 16:18:42
date last changed
2016-12-19 15:42:55
@article{4411554,
  abstract     = {Analysis of drugs and metabolites in biological matrices such as blood or plasma by LC-MS is routinely challenged by the presence of large quantities of competing molecules for ionization in soft ionization sources, such as proteins and phospholipids. While the former can easily be removed by protein precipitation, pre-analytical extraction of the latter is necessary because they show very high retention in reversed-phase LC resulting in long analysis times or in ion suppression effects when not eluted before the next runs. A novel HILIC-based SPE approach, making use of silica cartridges and of acetone as organic solvent, is introduced as a potent alternative to current commercial methods for phospholipid removal. The methodology was developed and tested for a broad polarity range of pharmaceutical solutes (log P from 0 to 6.6) and broad applicability can therefore be envisaged.},
  author       = {Van Damme, Thomas and Lachova, Miroslava and Lynen, Frederic and Szucs, Roman and Sandra, Patrick},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {Acetone as eluting solvent,HILIC SPE,TANDEM MASS-SPECTROMETRY,Phospholipid removal,Ion suppression,Matrix effects,LC-MS,INTERNAL STANDARD,TRIFLUOROACETIC-ACID,ION-PAIRING REAGENTS,HUMAN PLASMA,ELECTROSPRAY-IONIZATION,ESI-MS,SIGNAL SUPPRESSION,SAMPLE PREPARATION,PROTEIN PRECIPITATION},
  language     = {eng},
  number       = {2},
  pages        = {401--407},
  title        = {Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC-MS},
  url          = {http://dx.doi.org/10.1007/s00216-013-7281-7},
  volume       = {406},
  year         = {2014},
}

Chicago
Van Damme, Thomas, Miroslava Lachova, Frederic Lynen, Roman Szucs, and Patrick Sandra. 2014. “Solid-phase Extraction Based on Hydrophilic Interaction Liquid Chromatography with Acetone as Eluent for Eliminating Matrix Effects in the Analysis of Biological Fluids by LC-MS.” Analytical and Bioanalytical Chemistry 406 (2): 401–407.
APA
Van Damme, Thomas, Lachova, M., Lynen, F., Szucs, R., & Sandra, P. (2014). Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC-MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 406(2), 401–407.
Vancouver
1.
Van Damme T, Lachova M, Lynen F, Szucs R, Sandra P. Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC-MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2014;406(2):401–7.
MLA
Van Damme, Thomas, Miroslava Lachova, Frederic Lynen, et al. “Solid-phase Extraction Based on Hydrophilic Interaction Liquid Chromatography with Acetone as Eluent for Eliminating Matrix Effects in the Analysis of Biological Fluids by LC-MS.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 406.2 (2014): 401–407. Print.