Advanced search
1 file | 736.02 KB

Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC-MS

Thomas Van Damme (UGent), Miroslava Lachova (UGent), Frederic Lynen (UGent), Roman Szucs (UGent) and Patrick Sandra (UGent)
Author
Organization
Abstract
Analysis of drugs and metabolites in biological matrices such as blood or plasma by LC-MS is routinely challenged by the presence of large quantities of competing molecules for ionization in soft ionization sources, such as proteins and phospholipids. While the former can easily be removed by protein precipitation, pre-analytical extraction of the latter is necessary because they show very high retention in reversed-phase LC resulting in long analysis times or in ion suppression effects when not eluted before the next runs. A novel HILIC-based SPE approach, making use of silica cartridges and of acetone as organic solvent, is introduced as a potent alternative to current commercial methods for phospholipid removal. The methodology was developed and tested for a broad polarity range of pharmaceutical solutes (log P from 0 to 6.6) and broad applicability can therefore be envisaged.
Keywords
Acetone as eluting solvent, HILIC SPE, TANDEM MASS-SPECTROMETRY, Phospholipid removal, Ion suppression, Matrix effects, LC-MS, INTERNAL STANDARD, TRIFLUOROACETIC-ACID, ION-PAIRING REAGENTS, HUMAN PLASMA, ELECTROSPRAY-IONIZATION, ESI-MS, SIGNAL SUPPRESSION, SAMPLE PREPARATION, PROTEIN PRECIPITATION

Downloads

    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 736.02 KB

Citation

Please use this url to cite or link to this publication:

Chicago
Van Damme, Thomas, Miroslava Lachova, Frederic Lynen, Roman Szucs, and Patrick Sandra. 2014. “Solid-phase Extraction Based on Hydrophilic Interaction Liquid Chromatography with Acetone as Eluent for Eliminating Matrix Effects in the Analysis of Biological Fluids by LC-MS.” Analytical and Bioanalytical Chemistry 406 (2): 401–407.
APA
Van Damme, Thomas, Lachova, M., Lynen, F., Szucs, R., & Sandra, P. (2014). Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC-MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 406(2), 401–407.
Vancouver
1.
Van Damme T, Lachova M, Lynen F, Szucs R, Sandra P. Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC-MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2014;406(2):401–7.
MLA
Van Damme, Thomas, Miroslava Lachova, Frederic Lynen, et al. “Solid-phase Extraction Based on Hydrophilic Interaction Liquid Chromatography with Acetone as Eluent for Eliminating Matrix Effects in the Analysis of Biological Fluids by LC-MS.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 406.2 (2014): 401–407. Print.
@article{4411554,
  abstract     = {Analysis of drugs and metabolites in biological matrices such as blood or plasma by LC-MS is routinely challenged by the presence of large quantities of competing molecules for ionization in soft ionization sources, such as proteins and phospholipids. While the former can easily be removed by protein precipitation, pre-analytical extraction of the latter is necessary because they show very high retention in reversed-phase LC resulting in long analysis times or in ion suppression effects when not eluted before the next runs. A novel HILIC-based SPE approach, making use of silica cartridges and of acetone as organic solvent, is introduced as a potent alternative to current commercial methods for phospholipid removal. The methodology was developed and tested for a broad polarity range of pharmaceutical solutes (log P from 0 to 6.6) and broad applicability can therefore be envisaged.},
  author       = {Van Damme, Thomas and Lachova, Miroslava and Lynen, Frederic and Szucs, Roman and Sandra, Patrick},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keyword      = {Acetone as eluting solvent,HILIC SPE,TANDEM MASS-SPECTROMETRY,Phospholipid removal,Ion suppression,Matrix effects,LC-MS,INTERNAL STANDARD,TRIFLUOROACETIC-ACID,ION-PAIRING REAGENTS,HUMAN PLASMA,ELECTROSPRAY-IONIZATION,ESI-MS,SIGNAL SUPPRESSION,SAMPLE PREPARATION,PROTEIN PRECIPITATION},
  language     = {eng},
  number       = {2},
  pages        = {401--407},
  title        = {Solid-phase extraction based on hydrophilic interaction liquid chromatography with acetone as eluent for eliminating matrix effects in the analysis of biological fluids by LC-MS},
  url          = {http://dx.doi.org/10.1007/s00216-013-7281-7},
  volume       = {406},
  year         = {2014},
}

Altmetric
View in Altmetric
Web of Science
Times cited: