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MLVA as a tool for public health surveillance of human Salmonella Typhimurium : prospective study in Belgium and evaluation of MLVA loci stability

(2013) PLOS ONE. 8(12).
Author
Organization
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Bioinformatics: from nucleotids to networks (N2N)
Abstract
Surveillance of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (MLVA), which allow earlier detection and confinement of outbreaks. Here, a surveillance study, including phage typing, antimicrobial susceptibility testing and the in Europe most commonly used 5-loci MLVA on 1,420 S. Typhimurium isolates collected between 2010 and 2012 in Belgium, was used to evaluate the added value of MLVA for public health surveillance. Phage types DT193, DT195, DT120, DT104, DT12 and U302 dominate the Belgian S. Typhimurium population. A combined resistance to ampicillin, streptomycin, sulphonamides and tetracycline (ASSuT) with or without additional resistances was observed for 42.5% of the isolates. 414 different MLVA profiles were detected, of which 14 frequent profiles included 44.4% of the S. Typhimurium population. During a serial passage experiment on selected isolates to investigate the in vitro stability of the 5 MLVA loci, variations over time were observed for loci STTR6, STTR10, STTR5 and STTR9. This study demonstrates that MLVA improves public health surveillance of S. Typhimurium. However, the 5-loci MLVA should be complemented with other subtyping methods for investigation of possible outbreaks with frequent MLVA profiles. Also, variability in these MLVA loci should be taken into account when investigating extended outbreaks and studying dynamics over longer periods.
Keywords
ENTERICA SEROVAR TYPHIMURIUM, ELECTROPHORESIS, IBCN, TANDEM-REPEAT ANALYSIS, VARIABLE-NUMBER, ANTIMICROBIAL RESISTANCE, INFECTIONS, PHAGE TYPE, SEROTYPE TYPHIMURIUM, OUTBREAK

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Citation

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Chicago
Wuyts, Véronique, Wesley Mattheus, Guillaume De Laminne de Bex, Christa Wildemauwe, Nancy HC Roosens, Kathleen Marchal, Sigrid CJ De Keersmaecker, and Sophie Bertrand. 2013. “MLVA as a Tool for Public Health Surveillance of Human Salmonella Typhimurium : Prospective Study in Belgium and Evaluation of MLVA Loci Stability.” Plos One 8 (12).
APA
Wuyts, V., Mattheus, W., De Laminne de Bex, G., Wildemauwe, C., Roosens, N. H., Marchal, K., De Keersmaecker, S. C., et al. (2013). MLVA as a tool for public health surveillance of human Salmonella Typhimurium : prospective study in Belgium and evaluation of MLVA loci stability. PLOS ONE, 8(12).
Vancouver
1.
Wuyts V, Mattheus W, De Laminne de Bex G, Wildemauwe C, Roosens NH, Marchal K, et al. MLVA as a tool for public health surveillance of human Salmonella Typhimurium : prospective study in Belgium and evaluation of MLVA loci stability. PLOS ONE. 2013;8(12).
MLA
Wuyts, Véronique, Wesley Mattheus, Guillaume De Laminne de Bex, et al. “MLVA as a Tool for Public Health Surveillance of Human Salmonella Typhimurium : Prospective Study in Belgium and Evaluation of MLVA Loci Stability.” PLOS ONE 8.12 (2013): n. pag. Print.
@article{4410365,
  abstract     = {Surveillance of Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) is generally considered to benefit from molecular techniques like multiple-locus variable-number of tandem repeats analysis (MLVA), which allow earlier detection and confinement of outbreaks. Here, a surveillance study, including phage typing, antimicrobial susceptibility testing and the in Europe most commonly used 5-loci MLVA on 1,420 S. Typhimurium isolates collected between 2010 and 2012 in Belgium, was used to evaluate the added value of MLVA for public health surveillance. Phage types DT193, DT195, DT120, DT104, DT12 and U302 dominate the Belgian S. Typhimurium population. A combined resistance to ampicillin, streptomycin, sulphonamides and tetracycline (ASSuT) with or without additional resistances was observed for 42.5\% of the isolates. 414 different MLVA profiles were detected, of which 14 frequent profiles included 44.4\% of the S. Typhimurium population. During a serial passage experiment on selected isolates to investigate the in vitro stability of the 5 MLVA loci, variations over time were observed for loci STTR6, STTR10, STTR5 and STTR9. This study demonstrates that MLVA improves public health surveillance of S. Typhimurium. However, the 5-loci MLVA should be complemented with other subtyping methods for investigation of possible outbreaks with frequent MLVA profiles. Also, variability in these MLVA loci should be taken into account when investigating extended outbreaks and studying dynamics over longer periods.},
  articleno    = {e84055},
  author       = {Wuyts, V{\'e}ronique and Mattheus, Wesley and De Laminne de Bex, Guillaume and Wildemauwe, Christa and Roosens, Nancy HC and Marchal, Kathleen and De Keersmaecker, Sigrid CJ and Bertrand, Sophie},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {ENTERICA SEROVAR TYPHIMURIUM,ELECTROPHORESIS,IBCN,TANDEM-REPEAT ANALYSIS,VARIABLE-NUMBER,ANTIMICROBIAL RESISTANCE,INFECTIONS,PHAGE TYPE,SEROTYPE TYPHIMURIUM,OUTBREAK},
  language     = {eng},
  number       = {12},
  pages        = {10},
  title        = {MLVA as a tool for public health surveillance of human Salmonella Typhimurium : prospective study in Belgium and evaluation of MLVA loci stability},
  url          = {http://dx.doi.org/10.1371/journal.pone.0084055},
  volume       = {8},
  year         = {2013},
}

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